Essential Zebrafish Methods: Cell and Developmental Biology

Essential Zebrafish Methods: Cell and Developmental Biology

1st Edition - August 27, 2009

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  • Editors: Monte Westerfield, Leonard Zon, H. Detrich, III
  • eBook ISBN: 9780080923437
  • Paperback ISBN: 9780123745996

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Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and “tricks of the trade” to both experienced researchers and more junior members in the lab.

Key Features

  • Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors
  • Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error


Researchers in cell, developmental, and molecular biology; genetics

Table of Contents

  • Overview of the Zebrafish System
    H. William Detrich III
    Cell Cycles and Development in the Embryonic Zebrafish
    Donald A. Kane
    Primary Fibroblast Cell Culture
    Barry H. Paw
    Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)
    John H. Postlethwait
    Analysis of Protein and Gene Expression
    Trevor Jowett
    Analysis of Zebrafish Development Using Explant Culture Assays
    Hazel Sive
    Confocal Microscopic Analysis of Morphogenetic Movements
    Mark S. Cooper
    Cytoskeletal Dynamics of the Zebrafish Embryo
    Lilianna Solinica-Krezel
    Haploid Screens and Gamma-Ray Mutagenesis
    Charline Walker
    Positional Cloning of Mutated Zebrafish Genes
    Will Talbot
    The Zebrafish Genome
    John Postlethwait
    Analyzing Axon Guidance in the Zebrafish Retinotectal System
    Chi-Bin Chien
    Analysis of the Cell Cycle in Zebrafish Embryos
    James F. Amatruda
    Cellular Dissection of Zebrafish Hematopoiesis
    David Traver
    Culture of Embryonic Stem Cell Lines from Zebrafish
    Paul Collodi
    Laure Bally-Cuif
    Time-Lapse Microscopy of Brain Development
    Scott E. Fraser
    Development of the Peripheral Sympathetic Nervous System in Zebrafish
    Paul D. Henion
    Approaches to Study Neurogenesis in the Zebrafish Retina
    Jarema Malicki
    Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms
    David W. Tank
    Development of Cartilage and Bone
    Thomas F. Schilling
    Morphogenesis of the Jaw: Development Beyond the Embryo
    Pamela C. Yelick
    Cardiac Development
    Didier Y. R. Stainier
    Zebrafish Kidney Development
    Iain A. Drummond
    Retroviral-Mediated Insertional Mutogenesis in Zebrafish
    Adam Amsterdam
    Genetic Screens for Maternal-Effect Mutations
    Francisco Pelegri
    Behavior Screening Assays in Zebrafish
    Herwig Baier
    A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish
    Cecelia B. Moens
    Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element
    Koichi Kawakami
    Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in Zebrafish
    Charles Lee
    The Zon Laboratory Guide to Positional Cloning in Zebrafish
    Leonard I. Zon
    Sleeping Beauty Transposon for Efficient Gene Delivery
    Stephen C. Ecker
    Transgene Manipulation in Zebrafish by Using Recombinases
    Gary W. Stuart
    Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI
    Joachim Wittbrodt
    Cloning Zebrafish by Nuclear Transfer
    Shuo Lin
    Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale In Situ Hybridization Screening
    Christine Thisse
    Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish
    Bill Trevarrow
    Common Diseases of Laboratory Zebrafish
    Jennifer L. Matthews
    Zebrafish Sperm Cryopreservation
    Thomas Look

Product details

  • No. of pages: 584
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: August 27, 2009
  • Imprint: Academic Press
  • eBook ISBN: 9780080923437
  • Paperback ISBN: 9780123745996

About the Editors

Monte Westerfield

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Affiliations and Expertise

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Leonard Zon

Affiliations and Expertise

Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA

H. Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Professor of Biochemistry and Marine Biology at Northeastern University

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