Description

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development.
This book, one of two new volumes in the Reliable Lab Solutions series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world’s leading scientists. Culled from previously published chapters in Methods in Cell Biology and updated by the original authors where relevant, it provides a comprehensive collection of protocols describing the most widely used techniques relevant to the study of the cellular and developmental biology of zebrafish. The methods in this volume were hand-selected by the editors, whose goal was to a provide a handy and cost-effective collection of fail-safe methods, tips, and “tricks of the trade” to both experienced researchers and more junior members in the lab.

Key Features

* Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors
* Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error

Readership

Researchers in cell, developmental, and molecular biology; genetics

Table of Contents

Overview of the Zebrafish System H. William Detrich III Cell Cycles and Development in the Embryonic Zebrafish Donald A. Kane Primary Fibroblast Cell Culture Barry H. Paw Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization) John H. Postlethwait Analysis of Protein and Gene Expression Trevor Jowett Analysis of Zebrafish Development Using Explant Culture Assays Hazel Sive Confocal Microscopic Analysis of Morphogenetic Movements Mark S. Cooper Cytoskeletal Dynamics of the Zebrafish Embryo Lilianna Solinica-Krezel Haploid Screens and Gamma-Ray Mutagenesis Charline Walker Positional Cloning of Mutated Zebrafish Genes Will Talbot The Zebrafish Genome John Postlethwait Analyzing Axon Guidance in the Zebrafish Retinotectal System Chi-Bin Chien Analysis of the Cell Cycle in Zebrafish Embryos James F. Amatruda Cellular Dissection of Zebrafish Hematopoiesis David Traver Culture of Embryonic Stem Cell Lines from Zebrafish Paul Collodi Neurogenesis Laure Bally-Cuif Time-Lapse Microscopy of Brain Development Scott E. Fraser Development of the Peripheral Sympathetic Nervous System in Zebrafish Paul D. Henion Approaches to Study Neurogenesis in the Zebrafish Retina Jarema Malicki Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms David W. Tank Development of Cartilage and Bone Thomas F. Schilling Morphogenesis of the Jaw: Development Beyond the

Details

No. of pages:
584
Language:
English
Copyright:
© 2009
Published:
Imprint:
Academic Press
eBook ISBN:
9780080923437
Print ISBN:
9780123745996

About the editors

Monte Westerfield

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Affiliations and Expertise

Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Leonard Zon

Affiliations and Expertise

Howard Hughes Medical Institute, Children's Hospital, Boston, MA, USA

H. William Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Northeastern University, Boston, MA, USA