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Enzyme Immunodiagnosis - 1st Edition - ISBN: 9780124297456, 9780323159173

Enzyme Immunodiagnosis

1st Edition

Author: Edouard Kurstak
eBook ISBN: 9780323159173
Hardcover ISBN: 9780124297456
Paperback ISBN: 9780124336025
Imprint: Academic Press
Published Date: 15th July 1986
Page Count: 248
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Enzyme Immunodiagnosis focuses on the applications of enzyme immunodiagnosis procedures to the detection of drugs, infectious disease pathogens, and antigens in cancer pathology. This book discusses the use of immunoassays in vaccine assessment and standardization, including the application of commercial enzyme immunoassays (EIA) kits, automation of assays, and interpretation of results obtained with both serological and immunohistochemical methods. The developments in immunoblotting applications to diagnosis and research and use of polyclonal or monoclonal antibodies in enzyme immunodiagnosis are also reviewed. This book is beneficial to students and specialists conducting work on immunoenzymatic technology, as well as those involved in research, development, teaching, and diagnosis in fields in immunoassays.

Table of Contents


1 Introduction


2 Preparation of Immunoglobulins, Antibodies, or Fab Fragments

A. Immunization

B. Isolation of Immunoglobulins

C. Preparation of Fab Fragments

3 Conjugation of Enzymes

A. Purification of Enzymes

B. Selected Procedures of Conjugation

4 Principles of the Design of Enzyme Immunoassays

A. Formulation of Methodology

B. Solid Phases Used in Enzyme Immunoassays

C. Operational Details of Enzyme Immunoassay Procedures

D. Interpretation of Results Obtained with Enzyme Immunoassays

5 Enzyme Immunohistochemical Methods

A. Introduction

B. Fixation of Tissue

C. Detection of Antigens at the Light Microscopic Level

D. Double Immunoenzymatic Labeling

E. Nonspecific Staining

F. Ultrastructural Localization of Antigens

6 Enzyme Immunoassays After Immunoblotting

A. Technical Approaches

B. Immunoblotting Applications in Diagnosis and Research

C. Conclusion

7 The Use of Polyclonal or Monoclonal Antibodies in Enzyme Immunoassays

A. Variation in Immune Response

B. The Concepts of Affinity, Avidity, Cross-Reactivity, and Specificity

C. The Consequences of the Production of Monoclonal Antibodies for Affinity and Specificity

D. Unusual Properties of Monoclonal Antibodies

E. Conclusion

8 Homogeneous Enzyme Immunoassays for the Detection of Drugs

A. Introduction

B. Assay Design and Protocol

C. Qualitative and Semiquantitative Detection of Drugs

9 The Value of Enzyme Immunohistochemistry in Pathology, Oncology, and Histology

A. Enzyme Immunoassays in Diagnostic Pathology

B. Enzyme Immunohistochemical Detection of Antigens in Oncology

C. Immunoperoxidase Application in Histology

10 Enzyme Immunoassays for Infectious Diseases

A. Introduction

B. Enzyme Immunohistochemistry of Infected Cells and Tissue from Cultures

C. The Use of Polyclonal Antisera or Monoclonal Antibodies in Enzyme Immunoassays for Infectious Pathogens

D. Applications of Enzyme Immunoassays in Parasitology

E. Applications of EIA in Bacteriology, Chlamydiology, and Rickettsiology

F. Enzyme Immunoassays in Virology and for the Diagnosis of Viral Diseases

11 Automation in Enzyme Immunoassays and Commercial Kits

A. Total Automation

B. Semiautomation

C. Commercial Kits for Enzyme Immunoassays

12 Screening of Recombinant DNA Expression Libraries

A. Construction of Recombinant Transformants

B. Immunological Screening of Large cDNA Expression Libraries

13 Vaccine Assessment and Standardization

A. Safety and Efficacy of Prospective Vaccines

B. Vaccines: Quality Assessment and Standardization

14 Future Prospects of Enzyme Immunodiagnosis






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© Academic Press 1986
15th July 1986
Academic Press
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About the Author

Edouard Kurstak

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