COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Enzyme Engineering and Evolution: General Methods - 1st Edition - ISBN: 9780128211496, 9780128211502

Enzyme Engineering and Evolution: General Methods, Volume 643

1st Edition

Serial Volume Editor: Dan Tawfik
Hardcover ISBN: 9780128211496
eBook ISBN: 9780128211502
Imprint: Academic Press
Published Date: 3rd September 2020
Page Count: 360
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

1. In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis

Javier Viña-Gonzalez and Miguel Alcalde

2. A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering

Haoran Yu and Paul A. Dalby

3. Exploration of enzyme diversity: High-throughput techniques for protein production and microscale biochemical characterization

Michal Vasina, Pavel Vanacek, Jiri Damborsky and Zbynek Prokop

4. Emulsion-based directed evolution of enzymes and proteins in yeast

Elizabeth C. Gardner, Ella J. Watkins, Jimmy Gollihar and Andrew D. Ellington

5. Remodeling enzyme active sites by stepwise loop insertion

Md Anarul Hoque, Yong Zhang, Li Zhi, Li Cui and Yan Feng

6. Consensus Finder web tool to predict stabilizing substitutions in proteins

Bryan J. Jones, Chi Nok Enoch Kan, Christine Luo and Romas J. Kazlauskas

7. The use of consensus sequence information to engineer stability and activity in proteins

Matt Sternke, Katherine W. Tripp and Doug Barrick

8. Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme

Jung Min Choi and Hak-Sung Kim

9. Biological fitness landscapes by deep mutational scanning

Jacob D. Mehlhoff and Marc Ostermeier

10. Focused rational iterative site-specific mutagenesis (FRISM)

Danyang Li, Qi Wu and Manfred T. Reetz

11. Statistical analysis of mutational epistasis to reveal intramolecular interaction networks in proteins

Charlotte M. Miton, John Z. Chen, Kalum Ost, Dave W. Anderson and Nobuhiko Tokuriki

12. Machine learning-assisted enzyme engineering

Niklas E. Siedhoff, Ulrich Schwaneberg and Mehdi D. Davari

13. Ultrahigh throughput screening for enzyme function in droplets

Stefanie Neun, Paul J. Zurek, Tomasz S. Kaminski and Florian Hollfelder


This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


No. of pages:
© Academic Press 2020
3rd September 2020
Academic Press
Hardcover ISBN:
eBook ISBN:

Ratings and Reviews

About the Serial Volume Editor

Dan Tawfik

Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

Affiliations and Expertise

Principal Investigator, Department of Biomolecular Sciences, Weizmann Institute of Science, Israel