Enzyme Engineering and Evolution: General Methods

Enzyme Engineering and Evolution: General Methods

1st Edition - September 3, 2020

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  • Editor: Dan Tawfik
  • Hardcover ISBN: 9780128211496
  • eBook ISBN: 9780128211502

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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. In vivo site-directed recombination (SDR): An efficient tool to reveal beneficial epistasis

    Javier Viña-Gonzalez and Miguel Alcalde

    2. A beginner's guide to molecular dynamics simulations and the identification of cross-correlation networks for enzyme engineering

    Haoran Yu and Paul A. Dalby

    3. Exploration of enzyme diversity: High-throughput techniques for protein production and microscale biochemical characterization

    Michal Vasina, Pavel Vanacek, Jiri Damborsky and Zbynek Prokop

    4. Emulsion-based directed evolution of enzymes and proteins in yeast

    Elizabeth C. Gardner, Ella J. Watkins, Jimmy Gollihar and Andrew D. Ellington

    5. Remodeling enzyme active sites by stepwise loop insertion

    Md Anarul Hoque, Yong Zhang, Li Zhi, Li Cui and Yan Feng

    6. Consensus Finder web tool to predict stabilizing substitutions in proteins

    Bryan J. Jones, Chi Nok Enoch Kan, Christine Luo and Romas J. Kazlauskas

    7. The use of consensus sequence information to engineer stability and activity in proteins

    Matt Sternke, Katherine W. Tripp and Doug Barrick

    8. Structure-guided rational design of the substrate specificity and catalytic activity of an enzyme

    Jung Min Choi and Hak-Sung Kim

    9. Biological fitness landscapes by deep mutational scanning

    Jacob D. Mehlhoff and Marc Ostermeier

    10. Focused rational iterative site-specific mutagenesis (FRISM)

    Danyang Li, Qi Wu and Manfred T. Reetz

    11. Statistical analysis of mutational epistasis to reveal intramolecular interaction networks in proteins

    Charlotte M. Miton, John Z. Chen, Kalum Ost, Dave W. Anderson and Nobuhiko Tokuriki

    12. Machine learning-assisted enzyme engineering

    Niklas E. Siedhoff, Ulrich Schwaneberg and Mehdi D. Davari

    13. Ultrahigh throughput screening for enzyme function in droplets

    Stefanie Neun, Paul J. Zurek, Tomasz S. Kaminski and Florian Hollfelder

Product details

  • No. of pages: 360
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: September 3, 2020
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128211496
  • eBook ISBN: 9780128211502

About the Serial Volume Editor

Dan Tawfik

Dan Tawfik is Principal Investigator at The Department of Biomolecular Sciences, Weizmann Institute of Science, Israel.

Affiliations and Expertise

Principal Investigator, Department of Biomolecular Sciences, Weizmann Institute of Science, Israel

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