Endosome Signalling Part B, Volume 535

1st Edition

Serial Volume Editors: P. Michael Conn
Hardcover ISBN: 9780123979254
eBook ISBN: 9780123984814
Imprint: Academic Press
Published Date: 14th January 2014
Page Count: 518
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Table of Contents




Chapter One. Assessment of Insulin Proteolysis in Rat Liver Endosomes: Its Relationship to Intracellular Insulin Signaling


1 Introduction

2 Assays for Insulin Degradation

3 Assay for Endosomal Proteolysis of Insulin

4 Sites of Cleavage and Degradation Products of Insulin within Endosomes

5 Endosomal Insulinases

6 Effects of Insulin and Slowly Processed Insulin Analogs on Insulin Signaling in Liver Endosomes

7 Involvement of IR Endocytosis in Positive Regulation of Insulin Signaling


Chapter Two. A Bimolecular Fluorescent Complementation Screen Reveals Complex Roles of Endosomes in Ras-Mediated Signaling


1 Introduction

2 Detect Ras–effector Interactions by BiFC

3 Screen for Ras-binding Proteins by BiFC

4 Concluding Remarks



Chapter Three. TGFβ in Endosomal Signaling


1 Introduction

2 Endocytosis

3 The Role of the Early Endosome in TGFβ Signal Transduction

4 Readouts of TGFβ Signal Transduction



Chapter Four. Annexins and Endosomal Signaling


1 Introduction

2 Isolation of Endocytic Compartments

3 Targeting Raf-1 Signaling to Early Endosomes

4 Monitoring Endosomal Signaling by Fluorescence Resonance Energy Transfer Microscopy

5 Targeting Annexins to Endosomes and Other Cellular Compartments

6 Summary



Chapter Five. Analysis, Regulation, and Roles of Endosomal Phosphoinositides


1 Introduction

2 Endosomal PIs

3 Analysis of PtdIns3P Levels

4 Monitoring PtdIns3P Intracellular Localization

5 Endosomal PtdIns3P

6 Phospha


This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the second of two volumes on endosome signaling and includes chapters on such topics as measurement of entry into the endosomal compartment by multi-parametric image analysis, assessment of peptide internalization and endosomal signaling, and VEGF-A in endosomal signaling.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers endosome signaling
  • Contains chapters on such topics as measurement of biological effects of endosomal proteolysis of internalized insulin and multi-vesicular endosome biogenesis.


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


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© Academic Press 2014
Academic Press
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About the Serial Volume Editors

P. Michael Conn Serial Volume Editor

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA