Theory, Methods, and Applications

1st Edition - January 1, 1967

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  • Editor: Milan Bier
  • eBook ISBN: 9781483274294

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Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields of biochemistry, physiology, and medicine. The selection first offers information on the interpretation of electrophoretic mobilities, including theories for other models, electrophoresis of polyelectrolytes, and theory for a rigid spherical particle. The text then takes a look at primary protein structures and nomenclature and identification of the normal human serum proteins. Discussions focus on principles of nomenclature of the serum constituents; methods of identification of an isolated antigen; principal methods used to study serum proteins; separation of mixtures of peptides and amino acids by high-voltage electrophoresis; and methods for determining the primary structure of proteins. The publication elaborates on inheritance of protein variation in human serum and antibodies and myeloma proteins. Topics include products of enzymic digestion, products of reduction, naturally occurring fragments, genetic variation, and variations detected in human serum proteins. The manuscript then examines lymph and cerebrospinal fluid, electrophoresis of gastrointestinal secretions, and high resolution techniques. The selection is a dependable source of data for readers interested in electrophoresis.

Table of Contents

  • List of Contributors


    Contents of Volume I


    1. The Interpretation of Electrophoretic Mobilities

    I. Introduction

    II. General Considerations

    III. Theory for a Rigid Spherical Particle

    IV. Theories for Other Models

    V. Electrophoresis of Polyelectrolytes


    2. Primary Protein Structures

    I. General Conclusions from Present Knowledge of Primary Protein Structure

    II. Methods for Determining the Primary Structure of Proteins

    III. Separation of Mixtures of Peptides and Amino Acids by High-Voltage Electrophoresis


    3. Nomenclature and Identification of the Normal Human Serum Proteins

    I. Introduction

    II. Principal Methods Used to Study Serum Proteins

    III. Methods of Identification of an Isolated Antigen

    IV. Principles of Nomenclature of the Serum Constituents

    V. The Immunoglobulins

    VI. γ- and β2-Proteins Other Than Immunoglobulins

    VII. The β1-Globulins

    VIII. The α2-Globulins

    IX. The α1-Globulins

    X. Serum Albumin

    XI. The α0-Globulins

    XII. The Prealbumins (ρ-Proteins)

    XIII. The Lipoproteins

    XIV. The Serum Enzymes


    4. Inheritance of Protein Variation in Human Serum

    I. Genetic Variation

    II. Variations Detected in Human Serum Proteins


    5. Antibodies and Myeloma Proteins

    I. Introduction

    II. Whole Proteins

    III. Products of Enzymic Digestion

    IV. Products of Reduction

    V. Naturally Occurring Fragments

    VI. Concluding Remarks


    6. Lymph and Cerebrospinal Fluid

    I. Introduction

    II. Lymph

    III. Cerebrospinal Fluid


    7. Electrophoresis of Gastrointestinal Secretions

    I. Introduction

    II. Saliva

    III. Gastric Juice

    IV. Pancreatic Juice

    V. Duodenal and Jejunal Juice and Meconium

    VI. Bile


    8. High Resolution Techniques

    I. Introduction

    II. Analytical Methods

    III. Preparative Procedures

    IV. Photography of Gels

    V. Difficulties and Limitations


    9. Preparative Electrophoresis

    I. Introduction

    II. Electrophoresis in Homogeneous Media

    III. Zone Electrophoresis on Supporting Media

    IV. Continuous Zone Electrophoresis


    10. Automatic Density Gradient Electrophoresis

    I. Introduction

    11. Principles and Methods

    III. The Automatic Electrophoresis Apparatus


    11. Electrophoresis in Semifluid Films

    I. Introduction

    II. Procedures and Control of Critical Factors

    III. Applications

    IV. Detection and Quantitation by Far Ultraviolet Photometry

    V. The Spectrophor


    Author Index

    Subject Index

Product details

  • No. of pages: 572
  • Language: English
  • Copyright: © Academic Press 1967
  • Published: January 1, 1967
  • Imprint: Academic Press
  • eBook ISBN: 9781483274294

About the Editor

Milan Bier

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