Electrophoresis: Theory, Methods, and Applications, Volume II focuses on the contributions of electrophoresis in the advancement of knowledge on proteins, as well as in the fields of biochemistry, physiology, and medicine.
The selection first offers information on the interpretation of electrophoretic mobilities, including theories for other models, electrophoresis of polyelectrolytes, and theory for a rigid spherical particle. The text then takes a look at primary protein structures and nomenclature and identification of the normal human serum proteins. Discussions focus on principles of nomenclature of the serum constituents; methods of identification of an isolated antigen; principal methods used to study serum proteins; separation of mixtures of peptides and amino acids by high-voltage electrophoresis; and methods for determining the primary structure of proteins.
The publication elaborates on inheritance of protein variation in human serum and antibodies and myeloma proteins. Topics include products of enzymic digestion, products of reduction, naturally occurring fragments, genetic variation, and variations detected in human serum proteins. The manuscript then examines lymph and cerebrospinal fluid, electrophoresis of gastrointestinal secretions, and high resolution techniques.
The selection is a dependable source of data for readers interested in electrophoresis.
List of Contributors Preface Contents of Volume I Introduction
- The Interpretation of Electrophoretic Mobilities I. Introduction II. General Considerations III. Theory for a Rigid Spherical Particle IV. Theories for Other Models V. Electrophoresis of Polyelectrolytes References
- Primary Protein Structures I. General Conclusions from Present Knowledge of Primary Protein Structure II. Methods for Determining the Primary Structure of Proteins III. Separation of Mixtures of Peptides and Amino Acids by High-Voltage Electrophoresis References
- Nomenclature and Identification of the Normal Human Serum Proteins I. Introduction II. Principal Methods Used to Study Serum Proteins III. Methods of Identification of an Isolated Antigen IV. Principles of Nomenclature of the Serum Constituents V. The Immunoglobulins VI. γ- and β2-Proteins Other Than Immunoglobulins VII. The β1-Globulins VIII. The α2-Globulins IX. The α1-Globulins X. Serum Albumin XI. The α0-Globulins XII. The Prealbumins (ρ-Proteins) XIII. The Lipoproteins XIV. The Serum Enzymes References
- Inheritance of Protein Variation in Human Serum I. Genetic Variation II. Variations Detected in Human Serum Proteins References
- Antibodies and Myeloma Proteins I. Introduction II. Whole Proteins III. Products of Enzymic Digestion IV. Products of Reduction V. Naturally Occurring Fragments VI. Concluding Remarks References
- Lymph and Cerebrospinal Fluid I. Introduction II. Lymph III. Cerebrospinal Fluid References
- Electrophoresis of Gastrointestinal Secretions I. Introduction II. Saliva III. Gastric Juice IV. Pancreatic Juice V. Duodenal and Jejunal Juice and Meconium VI. Bile References
- High Resolution Techniques I. Introduction II. Analytical Methods III. Preparative Procedures IV. Photography of Gels V. Difficulties and Limi
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- © Academic Press 1967
- 1st January 1967
- Academic Press
- eBook ISBN: