This volume emphasizes the intracellular consequences of DNA damage, describing procedures for analysis of checkpoint responses, DNA repair in vivo, replication fork encounter of DNA damage, as well as biological methods for analysis of mutation production and chromosome rearrangements. It also describes molecular methods for analysis of a number of genome maintenance activities including DNA ligases, helicases, and single-strand binding proteins.

Key Features

*Part B of a 2-part series *Addresses DNA maintenance enzymes *Discusses damage signaling *Presents In vivo analysis of DNA repair *Covers mutation and chromosome rearrangements


Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Table of Contents

Table of Contents Chapter 1 Overproduction and purification of RFC-related clamp loaders and PCNA-related clamps from Saccharomyces cerevisiae Chapter 2 Functional Assays for Replication Protein A (RPA) Chapter 3 Human DNA Ligases I, III, and IV – purification and new specific assays for these enzymes. Chapter 4 Enzymatic Mechanism of the WRN Helicase/Nuclease Chapter 5 Analysis of the DNA Unwinding Activity of RECQ Family Helicases Chapter 6 Characterization of Checkpoints Responses to DNA damage in Saccharomyces cerevisiae: Basic Protocols Chapter 7 Recruitment of ATR-ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage Chapter 8 Multiple approaches to study S. cerevisiae Rad9, a prototypical checkpoint protein Chapter 9 Methods for studying adaptation to the DNA damage checkpoint in yeast Chapter 10 DNA damage-induced phosphorylation of Rad55 protein as a sentinel for DNA damage checkpoint activation in S. cerevisiae Chapter 11 Methods for studying Mutagenesis and Checkpoints in Schizosaccharomyces pombe Chapter 12 Methods for Determining Spontaneous Mutation Rates Chapter 13 Genomic Approaches for Identifying DNA Damage Response Pathways in Saccharomyces cerevisiae Chapter 14 Techniques for y-H2AX detection Chapter 15 Methods for studying the cellular response to DNA damage: influence of the Mre11 complex on chromosome metabolism Chapter 16 Detecting repair intermediates in vivo: effects of DNA damage response genes on single stranded DNA accumulation at uncapped telomeres in budding yeast


No. of pages:
© 2006
Academic Press
eBook ISBN:
Print ISBN:

About the serial-volume-editors

Judith Campbell

Affiliations and Expertise

California Institute of Technology, Pasadena, California, U.S.A.

Paul Modrich

Affiliations and Expertise

Department of Biochemistry, Duke University Medical Center, Durham, North Carolina, USA