DNA Repair Mechanisms

DNA Repair Mechanisms

1st Edition - January 28, 1978

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  • Editor: Philip Hanawalt
  • eBook ISBN: 9780323142328

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DNA Repair Mechanisms is an account of the proceedings at a major international conference on DNA Repair Mechanisms held at Keystone, Colorado on February 1978. The conference discusses through plenary sessions the overall standpoint of DNA repair. The papers presented and other important documents, such as short summaries by the workshop session conveners, comprise this book. The compilation describes the opposing views, those that agree and dispute about certain topic areas. This book, divided into 15 parts, is arranged according to the proceedings in the conference. The plenary sessions are grouped with the related workshop and poster manuscripts. The first two parts generally tackle repair in terms of its identification and quantification, as well as the models, systems, and perspectives it utilizes. The following parts discuss the various types of repair including base excision, nucleotide excision repair in bacteria, excision repair in mammalian cells, inducible/error-prone repair in prokaryotes, and strand break repair in mammalian cells among others. This reference material looks into the replicative bypass mechanisms in mammalian cells, viral probes, and hereditary repair defects. It explains repair deficiency and human disease, as well as mutagenesis and carcinogenesis. The last part of this book deals with the consequences and effects of DNA repair. This volume is a helpful source of reference for students, teachers, scientists, and researchers in the different fields of genetics, radiology, biochemistry, and environmental biology.

Table of Contents

  • Preface

    I. Repairable Damage: Identification and Quantification

    1. Repairable Damage in DNA

    2. Distribution and Quantitation of DNA Damage: Workshop Summary

    3. Lesions in Alkylated DNA Determined by Susceptibility to Alkali, Apurinic Endonuclease, or N-Glycosidase

    4. Enzymatic Recognition of DNA Damages Induced by Ionizing Radiation

    5. Micrococcus luteus UV-Endonuclease-Sensitive Sites in Far- and Near-UV-Irradiated Chinese Hamster Ovary Cells

    6. Repair of Alkylated DNA in Chinese Hamster Cells Measured by Loss of Enzyme-Sensitive Sites in Isolated DNA

    7. Serologic Assay of DNA Base Damage

    8. Non-dimer UV Damage in DNA and Poly d(A-T)

    9. Photochemistry and Photobiology of DNA Containing 5-Iodouracil and 5-Iodocytosine

    II. Repair Pathways: Models, Systems, and Perspectives

    10. DNA Repair Pathways: Excision Repair in Permeabilized Cells

    11. A New Classification of Pathways Repairing Pyrimidine Dimer Damage in DNA

    12. Making and Correcting Errors in DNA Synthesis: In Vitro Studies of Mutagenesis

    13. Cell Survival, Excision Repair, and DNA Replication in Eukaryotic Cells

    14. Electron Microscopy of UV-Induced Post Replication Repair Daughter Strand Gaps

    15. A General Method for Isolation of Repair-Deficient Mutants

    16. Historical Perspectives and Keynotes on DNA Repair

    III. Mechanism and Diversity of Enzymatic Photoreactivation

    17. Enzymatic Photoreactivation of DNA

    18. Mechanism and Diversity of Photoreactivation: Workshop Summary

    19. Some Properties of a DNA Photoreactivating Enzyme from Streptomyces griseus

    20. Low Molecular Weight Substances That Enhance DNA Photolyase Activity

    21. Mechanism of Action of the Photoreactivating Enzyme from E. coli: Recent Results

    22. Photosensitized Splitting of Thymine Dimers in DNA by Peptides and Proteins Containing Tryptophanyl Residues

    23. Evidence for Photoenzymatically Repairable, Lethal "Nondimer" Photoproducts Formed in E. coli Cells by Near UV Light

    24. Photoreactivation of Escherichia coli Irradiated with Ionizing Radiation

    25. Ultraviolet-Light Triggered Disappearance of Photoreactivating Enzyme

    26. Cloning the PHR Gene of Escherichia coli

    IV. Base Excicion Repair

    27. Base Excision Repair of DNA

    28. Enzymology of Base Excision Repair: Workshop Summary

    29. DNA Glycosylases of Escherichia coli

    30. Uracil-DNA Glycosylase Mutants Are Mutators

    31. Endonucleases Specific for Apurinic Sites in DNA

    32. The Endonuclease Activity of Exonuclease III and the Repair of Uracil-Containing DNA in Escherichia coli

    33. Base-Excision Repair in Micrococcus luteus

    34. Enzymes from Human Fibroblasts for the Repair of AP DNA

    V. Nucleotide Excision Repair in Bacteria

    35. Enzymatic Pathways of Damaged Nucleotide Excision

    36. Enzymology of Nucleotide Excision Repair

    37. A DNA-Binding Activity Associated with the uvrA + Protein from Escherichia coli

    38. Cloning of E. coli DNA Repair Genes

    39. Endonuclease Activities in Extracts of Micrococcus luteus Against Gamma-Irradiated DNA

    40. Excision Repair Pathways in Bacteria: Workshop Summary

    41. Excision Repair and Mutagenesis Are Complex Processes

    42. UVRD, UVRE, and RECL Represent a Single Gene

    43. Alternative Pathways for Excision and Resynthesis in Escherichia coli: DNA Polymerase III Role?

    44. Pyrimidine Dimer Excision in Exonuclease Deficient Mutants of Escherichia coli

    45. Conditions for Constitutive and Inducible Gap Filling of Excision and Postreplication Repair in Toluene Treated E. coli

    46. Effects of Phage Infection on Escherichia coli Excision Repair Measured in Vitro

    47. Repair of DNA Damage in Mycoplasmas

    48. Role of the Gene MTCA in the Resistance of Micrococcus radiodurans to the Lethal Effects of Mitomycin C and Alkylation Mutagenesis

    49. On the Mechanism for Repair of Cross-Linked DNA in E. coli Treated with Psoralen and Light

    VI. Excision Repair in Mammalian Cells

    50. Endonuclease from Calf Thymus That Acts on DNA Irradiated with Low UV Doses

    51. On the Nature of the Human Endonuclease Activity Directed Against Ultraviolet-Irradiated DNA

    52. Partial Purification and Characterization of Three Thymine Dimer Excising Activities from Human KB Cells

    53. Introduction of T4 Endonuclease V into Frozen and Thawed Mammalian Cells for the Determination of Removal of UV-Induced Photoproducts

    54. Removal of T4 Endonuclease V Sensitive Sites and Repair Replication in Confluent Human Diploid Fibroblasts

    55. Studies on the Molecular Mechanisms of Nucleotide Excision Repair in UV-Irradiated Human Cells in Culture

    56. Kinetics of UV-Induced Excision Repair in Human Fibroblasts. Simultaneous Studies of Incision-Produced Single-Strand Breaks and the Removal of

    Endonuclease-Sensitive Sites Using the DNA Unwinding Technique

    57. The Removal of UV-Induced Pyrimidine Dimers from the Replicated and Unreplicated DNA of Human Fibroblasts

    58. Distribution of UV-Induced DNA Repair Synthesis in Human Chromatin

    59. Excision Repair in Mammalian Cells

    60. A Possible Role for Poly ADP-Ribose in DNA Repair

    VII. Inducible/Error-Prone Repair in Procaryotes

    61. Conditioned Repair Responses: Workshop Summary

    62. The Involvement of E. coli DNA Polymerase III in Constitutive and Inducible Mutagenic Repair

    63. Studies of the recA and lex A Genes of Escherichia coli K-12

    64. An Escherichia coli Mutant with an Altered Inducible rec + llex+ Dependent DNA Repair Pathway

    65. Induction and Repression of the recA Gene of Escherichia coli

    66. Complexity in the Regulation of SOS Functions in Bacteria

    67. tif-1 Mediated Recovery from UV Blockage of DNA Synthesis in Escherichia coli: A Mechanism of Induced Mutagenesis?

    68. The Role of Proteases in SOS Regulation

    69. Transcriptional Regulation of the recA Region of E. coli

    70. Dose Response Relations for UV-Induced Repair Phenomena

    71. Pathways Involved in Repair of Alkylation Damage in E. coli

    72. The UV Inducibility of P32 Synthesis

    73. The Dependence of UV Mutagenesis in Phage T4 on DNA Ligase, Polymerase, and Nuclease Functions

    VIII. Repair in Lower Eucaryotes

    74. DNA Repair in Lower Eucaryotes: Workshop Summary

    75. Pathways of DNA Repair in Yeast

    76. The Importance of DNA Double-Strand Break Repair in Yeast

    77. On the Inducibility of Error-Prone Repair in Yeast

    78. The Fate of Mitochondrial DNA and of Mitochondrial Genetic Markers after UV Irradiation of Saccharomyces cerevisiae

    79. Recombination and Repair in Simple Eukaryotes

    80. Ultraviolet Light Induced Mutagenesis in Saccharomyces cerevisiae

    81. Regulation by Proteinases of a Putative /tec-Nuclease of Neurospora

    82. Genetics of DNA Repair in the Cellular Slime Mold, Dictyostelium discoideum

    83. DNA Repair in Drosophila

    84. Mutagen Sensitivity of Drosophila melanogaster IV. Interactions of X Chromosome Mutants

    IX. Strand Break Repair in Mammalian Cells

    85. DNA Strand Break Repair in Eukaryotes: Workshop Summary

    86. Estimation of DNA Strand Breaks in Single Mammalian Cells

    87. Repair of DNA Breaks Induced by Gamma Rays and Fast Neutrons in Chinese Hamster Cells

    88. A Relationship between DNA Single-Strand Breaks and DNA-Protein Crosslinks

    in Intercalator-Treated Mouse LI210 Cells

    89. DNA Damage and Mammalian Cell Killing

    90. Rejoining of DNA Strand Breaks in Nondividing Cells Irradiated in Situ

    X. Replicative Bypass Mechanisms in Mammalian Cells

    91. Replicative Bypass Mechanisms in Mammalian Cells: Workshop Summary

    92. DNA Replication in Ultraviolet-Irradiated Mammalian Cells

    93. Pyrmidine Dimers in DNA Strands of Mammalian Cells Synthesized after UV-Irradiation

    94. On the Presence of UV-Endonuclease Sensitive Sites in Daughter DNA of UV-Irradiated Mammalian Cells

    95. The Mechanism of Postreplication Repair in Mammalian Cells

    96. A Model for Postreplication Repair of UV Damage in Mammalian Cells

    97. The Kinetics of Postreplication Repair in Mammalian Cells as Studied by the Alkaline Elution Technique

    98. DNA Replication in Normal and Defective Human Cells after UV Irradiation

    99. Replicative Repair in Xeroderma pigmentosum (XP) Variants

    100. DNA Bifilarly Substituted with Bromodeoxyuridine in the First Round of Synthesis

    101. DNA Template Breakage and Decreased Excision of Hydro-Carbon Derived Adducts from Chinese Hamster Cell DNA Following Caffeine-Induced Inhibition

    of Postreplication Repair

    XI. Viral Probes for DNA Repair

    102. Viral Probes for Mammalian Cell DNA Repair: Results and Prospects: Workshop Summary

    103. Studies on UV-Induced Viral Reversion, Cockayne's Syndrome, and MNNG Damage Using Adenovirus 5

    104. Production of Viral Structural Antigens by Irradiated Adenovirus as an Assay for DNA Repair in Human Fibroblasts

    105. Simian Virus 40 as a Probe for Studying DNA Repair Pathways in Mammalian Cells

    106. Repair of UV Irradiation Damage in Simian Virus 40

    107. Defective Host-Cell Reactivation of UV-Irradiated Herpes Simplex Virus by Bloom's Syndrome Skin Fibroblasts

    108. Protease Inhibitors Prevent UV-Enhanced Virus Reactivation in E. coli but Not in Monkey Kidney Cells

    109. UV-Reactivation of Herpes Simplex Virus is Mutagenic and Inducible in Mammalian Cells

    110. The Effect of DNA Damage on the Induction of Simian Virus 40 (SV40) in Transformed Hamster Cells

    111. Recent Developments in the Biochemistry of Genetic Recombination

    112. In Initiation of Genetic Recombination in E. coli: Studies on Cutting in trans as a Function of Time

    113. Repair and Recombination of UV-Irradiated Phage Lambda

    XII. Hereditary Repair Defects in Man: Xeroderma Pigmentosum

    114. Xeroderma pigmentosum

    115. Xeroderma pigmentosum: Workshop Summary

    116. DNA Repair Nucleotide Sequences Which Prevent Premature Death of Neurons in Humans: Evidence from Studies on Xeroderma pigmentosum

    117. The Relationship between Neurologic Disease, Acute Sun Sensitivity, and Post-Ultraviolet Colony-Forming Ability in Xeroderma pigmentosum

    118. DNA Repair and Clinical Characteristics of 96 Xeroderma pigmentosum Patients in Japan

    119. Response of Mammalian Cells to Chemical Damage

    XIII. Repair Deficiency and Human Disease: Other Hereditary Defects

    120. DNA Repair Defects and Human Disease

    121. Cell Killing and Mutagenesis in Repair-Defective Human Cells

    122. Ataxia telangiectasia: A Model Inherited Disease Linking Deficient DNA Repair with Radiosensitivity and Cancer Proneness

    123. Ataxia telangiectasia: Characterization of Heterozygotes

    124. Repair Deficiency in N-Methyl-N'-Nitro-N-Nitrosoguanidine Treated Ataxia telangiectasia Fibroblasts

    125. An Investigation of DNA Repair Potential in Bloom's Syndrome

    126. Single-Strand DNA Breakage and Repair in Bloom's Syndrome Cells

    127. Effects of DNA Damaging Agents on Cultured Fibroblasts Derived from Patients with Cockayne Syndrome

    128. Sister Chromatid Exchanges in Dyskeratosis congenita after Exposure to Trimethyl Psoralen and UV Light

    129. Fanconi's Anemia a Condition Possibly Associated with a Defective DNA Repair

    130. X-Ray Sensitivity of Fibroblasts from Patients with Retinoblastoma and with Abnormalities of Chromosome 13

    XIV. Mutagenesis and Carcinogenesis

    131. Environmental Chemicals Causing Cancer and Genetic Birth Defects

    132. Chemical Damage and Mutagenesis in Mammalian Systems: Workshop Summary

    133. Biological Consequences of X-Ray-Induced DNA Damage and Repair Processes in Relation to Cell Killing and Carcinogenesis

    134. Chemical Modification of Radiation-Induced Mutagenesis and Its Role in Carcinogenesis

    135. Biological and Biochemical Evidence That DNA Repair Processes in Normal Human Cells Act to Reduce the Lethal and Mutagenic Effects of Exposure to Carcinogens

    136. Evidence for the Involvement of Different Repair Mechanisms in Mutagenesis and Cell Killing in V79 Cells

    137. Effects of Liquid Holding on Cell Killing and Mutation Induction in Normal and Repair-Deficient Human Cell Strains

    138. Fixation and Expression of Recessive Mutations in Mammalian Cells as a Model for the Study of Carcinogenesis

    139. Mammalian Cell Mutagenesis as a Biological Consequence of DNA Damage

    XV. Consequences of DNA Damage and Repair

    140. Relation between DNA Repair, Chromosome Aberrations, and Sister Chromatid Exchanges

    141. DNA Repair in Differentiation

    142. The Relationship between Cellular Aging and Genetic Defects

    143. The Acute and Chronic Effects of Ultraviolet Energy on Mammalian Skin

    144. Death of Escherichia coli Cells as a Consequence of Damage to DNA by Far-Ultraviolet Radiation

    145. Action of Three Mutagens on HeLa DNA Replication

    146. UV Irradiation and the Mammalian Cell Cycle

    147. Cloning of a Gene That Controls Radiation Sensitivity, Cell Division, and Capsular Polysaccharide Synthesis, the lon(capR) Gene

    Author Index

    Subject Index

Product details

  • No. of pages: 832
  • Language: English
  • Copyright: © Academic Press 1978
  • Published: January 28, 1978
  • Imprint: Academic Press
  • eBook ISBN: 9780323142328

About the Editor

Philip Hanawalt

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