Table of Contents

Series Page

Contributors

Preface

Chapter 1. Microscope Basics

Introduction

1.1 How Microscopes Work

1.2 Objective Basics

1.3 Mounting Video Cameras on the Microscope

Reference

Chapter 2. The Optics of Microscope Image Formation

Introduction

2.1 Physical Optics: The Superposition of Waves

2.2 Huygens’ Principle

2.3 Young’s Experiment: Two-Slit Interference

2.4 Diffraction from a Single Slit

2.5 The Airy Disk and the Issue of Microscope Resolution

2.6 Fourier or Reciprocal Space: The Concept of Spatial Frequencies

2.7 Resolution of the Microscope

2.8 Resolution and Contrast

Conclusions

2.9 Appendix A

2.10 Appendix B

2.11 Appendix C

Acknowledgments

References

Chapter 3. Proper Alignment of the Microscope

3.1 Key Components and Image Locations of the Light Microscope

3.2 Working the Microscope: Basic Setup

3.3 Addendum

Acknowledgments

Chapter 4. Fundamentals of Fluorescence and Fluorescence Microscopy

Introduction

4.1 Light Absorption and Beer’s Law

4.2 Atomic Fluorescence

4.3 Organic Molecular Fluorescence

4.4 Excited State Lifetime and Fluorescence Quantum Efficiency

4.5 Excited State Saturation

4.6 Nonradiative Decay Mechanisms

4.7 Fluorescence Resonance Energy

4.8 Fluorescence Depolarization

4.9 Measuring Fluorescence in the Steady State

4.10 Construction of a Monochromator

4.11 Construction of a Photomultiplier Tube

4.12 Measuring Fluorescence in the Time-Domain

4.13 Filters for the Selection of Wavelength

4.14 The Fluorescence Microscope

4.15 The Power of Fluorescence Microscopy

Acknowledgments

References

Chapter 5. Fluorescent Protein Applications in Microsc

Details

No. of pages:
672
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780124077614
Electronic ISBN:
9780124078925