Dictyostelium Discoideum - 1st Edition - ISBN: 9780124561502, 9780323159807

Dictyostelium Discoideum

1st Edition

A Developmental System

Authors: William Loomis
eBook ISBN: 9780323159807
Imprint: Academic Press
Published Date: 1st January 1975
Page Count: 224
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Dictyostelium Discoideum: A Developmental System presents D. discoideum as a model eukaryotic system to study a variety of developmental problems. The book describes the life cycle of the organism D. discoideum which exists in nature as a soil amoeba in forest detritus. The nuclei of D. discoideum contain DNA, RNA, and proteins which have properties similar to those of histones present in calf thymus nuclei. The unique sequences of the genome of the organism code for the gene products synthesize during its growth and development. One approach in genetic analyses to understanding the developmental processes of the organism is to isolate a large number of morphological mutants. The isolation process will indicate the number of variations that are open to the developing system, and will point to causal connections between stages. The text also explains that the number of cells which differentiate into stalk cells is the result of timing and mechanics of the process of culmination. Ashworth (1971) suggests that cells in pseudoplasmodia of D. discoideum adjust their positions in relation to the chemical structure of the sheath itself. A model shows how cells communicate their response to the sheath in an axial fashion. The book can prove beneficial for biochemists, micro-biologists, cellular researchers, and investigators involved in the study of cellular biology.

Table of Contents


Chapter 1 The Life Cycle

An Overview

Related Species

Chapter 2 Amoeboid Stage

Growth Conditions

Amoeboid Movement in D. Discoideum



Chapter 3 The Genome


Nuclear Composition

Mitochondrial DNA

Repetitive Sequences

Unique Sequences

Chapter 4 Genetics of Dictyostelium

Genetic Approaches

Mutational Genetics



Stock Maintenance

Chapter 5 Aggregation Stage


Periodic Movement

Cohesion of Developing Cells

Chapter 6 Pseudoplasmodial Stage


Polarity of Migration



Cell Sorting in Pseudoplasmodia

Chapter 7 Culmination Stage

Stalk Formation

Spore Formation

Avoidance Reaction

Germination Inhibitor


Mutational Analysis of Culmination

Chapter 8 Macromolecular Aspects of Development

Decrease in Dry Weight and Oxygen Consumption

Protein Content

RNA Content

Polysaccharide Content

DNA Content

Axenically Grown Cells

RNA Metabolism

Structure of Nuclear and Cytoplasmic RNA

Transcriptional Changes

Chapter 9 Enzymatic Aspects of Development

Enzymes of the Growth Phase

Enzymes of Growth and Development

Enzymes of Development

Marker Enzymes of Development

Enzyme Synthesis

Enzyme Degradation

Stage-Specific Enzymes

Mutational Analysis of Stage-Specific Enzymes

Chapter 10 Changes in Metabolites during Development

Cells Grown on Bacteria

Axenically Grown Cells

Chapter 11 The Relation of Biochemical Differentiations to Morphogenesis


Pseudoplasmodium Formation


Glycogen Metabolism

Substrate Concentrations

Metabolic Modeling

Genetic Analysis

Chapter 12 Modifications of Development

Submerged Cultures

Cell Density

Overhead Light


Mechanical Barriers

Effect of Nutrients

Phenocopies of Aggregateless Mutants

Phenocopies of Fruitless Mutants

Phenocopies of Culmination Mutants

Growth Conditions

Genetic Modifications


Chapter 13 Tissue Proportioning

Size Invariance

Mechanical Analysis

Differentiations of Prespore and Prestalk Cells

Cell Sorting and Determination

Regulation of Tissue Proportions

Positional Information

Cell Contact Model

Diffusional Barrier Model

Structural Model


Subject Index


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© Academic Press 1975
Academic Press
eBook ISBN:

About the Author

William Loomis

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