Description

Mycotoxins - toxic secondary metabolites produced by mycotoxigenic fungi – pose a significant risk to the food chain. Indeed, they may be the most hazardous of all food contaminants in terms of chronic toxicity and legislative limits on their levels in food and feed continue to be developed worldwide. Rapid and reliable methods for the determination of both mycotoxigenic fungi and mycotoxins in food and feed are therefore essential. This book reviews current and emerging methods in this area.

Part one focuses on the essentials of mycotoxin determination, covering sampling, sample preparation and clean-up and key determination techniques, such as chromatographic separation, liquid chromatography-mass spectrometry and immunochemical methods. Part two then goes on to describe quality assurance, official methods and performance criteria for determining mycotoxins in food and feed. Topics covered include laboratory accreditation, method validation and measurement uncertainty. The development and analysis of biomarkers for mycotoxins are discussed in part three. Individual chapters focus on detecting exposure in humans and animals. Part four is concerned with the processes involved in determining mycotoxigenic fungi in food and feed. It also describes the identification of genes and gene clusters involved in mycotoxin synthesis, as well as DNA barcoding of toxigenic fungi. Finally, part five explores some of the emerging methods for mycotoxin analysis, ranging from bio-sensing to spectroscopic techniques.

With its distinguished editor and international team of contributors, Determining mycotoxins and mycotoxigenic fungi in food and feed is a standard reference for all those concerned with reducing mycotoxin contamination in the food chain.

Key Features

  • Focuses on the essentials of mycotoxin determination, covering sampling, sample preparation, clean-up and key determination techniques
  • Documents quality assurance and official methods and performance criteria for determining mycotoxins in food and feed
  • Explores the processes of determining mycotoxigenic fungi in food and feed including the identification of genes and gene clusters

Readership

All those concerned with reducing mycotoxin contamination in the food chain

Table of Contents

Contributor contact details

Woodhead Publishing Series in Food Science, Technology and Nutrition

Preface

Part I: Determining mycotoxins in food and feed

Chapter 1: Sampling strategies to control mycotoxins

Abstract:

1.1 Food safety and the requirements for international food trade

1.2 Principles of food and feed sampling for mycotoxin analysis

1.3 International guidance on sampling food and feed for mycotoxin analysis

1.4 Uncertainty estimation and designing sound sampling plans for mycotoxin analysis in food and feed

1.5 Quality assurance and quality control procedures in sampling and arrival of the samples at the analytical laboratory

1.6 Strengthening national food control systems

Chapter 2: Sample preparation and clean up in mycotoxin analysis: principles, applications and recent developments

Abstract:

2.1 Introduction

2.2 Methods used for extraction and clean up of mycotoxins from complex matrices

2.3 Recent developments

2.4 Conclusions

2.5 Acknowledgements

Chapter 3: Chromatographic separation techniques for determination of mycotoxins in food and feed

Abstract:

3.1 Introduction

3.2 Thin-layer chromatography in mycotoxin analysis of food and feed

3.3 Gas chromatography in mycotoxin analysis of food and feed

3.4 High-performance liquid chromatography in mycotoxin analysis of food and feed

3.5 Electrophoretic separations in mycotoxin analysis of food and feed

3.6 Future trends

Chapter 4: Mass spectrometry in multi-mycotoxin and fungal spore analysis

Abstract:

4.1 Introduction

4.2 Liquid chromatography–mass spectrometry (LC–MS) methods in multi-mycotoxin analysis

4.3 Liquid chromatographic aspects of multi-mycotoxin methods

4.4 Mass spectrometric aspects of multi-mycotoxin methods

4.5

Details

No. of pages:
456
Language:
English
Copyright:
© 2011
Published:
Imprint:
Woodhead Publishing
Print ISBN:
9781845696740
Electronic ISBN:
9780857090973