Cytology and Cell Physiology

Cytology and Cell Physiology

3rd Edition - January 1, 1964

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  • Editor: Geoffrey Bourne
  • eBook ISBN: 9780323162012

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Cytology and Cell Physiology, Third Edition focuses on cell cytology and physiology as well as recent advances in the techniques in studying cells, including microscopy. It also describes cell membranes, surface, and physiology; cytoplasmic constituents; nucleus and nucleocytoplasmic reactions; enzyme histochemistry and cytochemistry; viruses within cells; and morphology of the cancer cell. Organized into 13 chapters, this edition begins with a historical overview of cytology, the template hypothesis of protein synthesis, and the respiratory function of mitochondria. It then discusses the subcellular components and their centrifugal isolation, some general principles of microscopy, selected physical and physicochemical methods, applications of enzyme histochemistry to electron microscopy, and structure and physical properties of the plasma membrane. The remaining chapters focus on the endoplasmic reticulum, the Golgi apparatus, the nucleus and its role in cell metabolism, RNA synthesis and movement, the behavior of viruses within cells, and pathological changes in cells. The book concludes with a chapter on the function and metabolism of cancer cells. This book is highly recommended to cytologists, investigators in the field of pathology, and graduate students in biology, biochemistry, physiology, and anatomy.

Table of Contents

  • List of Contributors


    Preface to First Edition

    Preface to Second Edition

    1 Introduction

    I. Historical Aspects of Cytology

    II. The Template Hypothesis of Protein Synthesis

    III. The Respiratory Function of Mitochondria


    2 Centrifugal Isolation of Subcellular Components

    I. Introduction

    II. Subcellular Organelles: Their Isolation, Composition, and Function

    III. Concluding Remarks


    3 Microscopy

    I. Introduction

    II. Some General Principles

    III. Special Methods of Microscopy

    IV. Conclusion


    4 Selected Physical and Physicochemical Methods

    I. Introduction

    II. Cytoplasmic Properties

    III. Electrical Properties of Cells

    IV. Analytical Methods


    5 Enzyme Histochemistry and Cytochemistry

    I. Introduction

    II. Fixation

    III. Phosphatases

    IV. Esterases

    V. Glycosidases

    VI. Transglycosylases

    VII. Proteolytic Enzymes

    VIII. Oxidases

    IX. Dehydrogenases

    X. Applications of Enzyme Histochemistry to Electron Microscopy


    6 The Cell Surface and Cell Physiology

    I. Introduction

    II. Structure and Physical Properties of the Plasma Membrane

    III. Fertilization, Artificial Parthenogenesis, Cell Division, and Protoplasmic Streaming


    7 The Endoplasmic Reticulum

    I. Definition

    II. General Description of α-Cytomembranes

    III. The Fine Structure of the α-Cytomembranes

    IV. The Occurrence of α-Cytomembranes and Ribosomes

    V. The α-Cytomembranes in the Living Cells

    VI. Chemistry of the α-Cytomembranes and Ribosomes

    VII. The Relationship between the α-Cytomembranes and Other Cytoplasmic Membranes

    VIII. Are the α-Cytomembranes Continuous with the Plasma Membrane?

    IX. The Topographical Relationship between α-Cytomembranes and the Mitochondria

    X. The Relationship between α-Cytomembranes and Cytoplasmic Vesicles

    XI. The Development of α-Cytomembranes

    XII. The Function of the α-Cytomembranes

    XIII. Historical Notes


    8 Mitochondria and the Golgi Complex

    I. Introduction

    II. Mitochondria

    III. The Golgi Apparatus


    9 The Nucleus and Chromosomes: A Cytological Perspective

    I. General

    II. Morphological and Chemical Description of the Nucleus

    III. The Chromosomes as Repositories of Genetic Information

    IV. Chromosome Structure and the Orderly Sequestering of Genetic Information

    V. Two Cases Reflecting the Cytology of Transcriber Function


    10 Nucleocytoplasmic Relationships

    I. Introduction

    II. Methods

    III. "Minor" Activities of the Nucleus

    IV. RNA Synthesis and Movement

    V. Protein Synthesis

    VI. Action of Cytoplasm (and Environment) on Nuclear Behavior and Metabolism

    VII. Relative Roles of Nucleus and Cytoplasm in the Heredity of the Cell

    VIII. The Nuclear Membrane as a Barrier between Nucleus and Cytoplasm

    IX. Hypothesis Concerning Machinery of Communication between Cytoplasm and Nucleus

    X. Conclusion


    11 Viruses and Cells

    I. Introduction

    II. The Anatomy of Viruses

    III. The Behavior of Viruses within Cells

    IV. Conclusions


    12 Pathological Changes in Cells

    I. Introduction

    II. Recent Discoveries about Cell Injury

    III. Subcellular Pathology; the Chemical Geography of the Injured Cell


    13 The Cancer Cell

    I. General Properties of Tumor Cells

    II. Morphology of the Cancer Cell

    III. Some Remarks about Function in the Cancer Cell

    IV. A Note on Exfoliative Cytology


    General References

    Author Index

    Subject Index

Product details

  • No. of pages: 798
  • Language: English
  • Copyright: © Academic Press 1964
  • Published: January 1, 1964
  • Imprint: Academic Press
  • eBook ISBN: 9780323162012

About the Editor

Geoffrey Bourne

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