Cytogenetics of Cells in Culture

Cytogenetics of Cells in Culture documents the proceedings of a symposium that aimed to not only establish guideposts to the direction of the most recent progress in the rapidly developing field of cybegenetics, but to cross-pollinate, in the best Mendelian tradition, the thinking of widely recognized leaders in this field with the current ideas of their colleagues, within the intimacy of this small meeting. The contributors to this symposium emphasized (1) new techniques to detect patterns of DNA replication, to better understand cellular control mechanisms, and to perform chromosome analyses with automated instrumentation; (2) the effects of cell hybridization and of noxious substances such as radiation, chemical agents, and viruses on chromosomal patterns; and (3) the influence of altered chromosomal complement on the survival of cells to long-term tissue culture or to ionizing radiation treatment.
It is hoped that the products of the many stimulating discussions which ensued during the 3-day gatherings will stimulate increased vigor and proliferation of research by the participants. It is further hoped that the publication of this volume will infect the reader with new concepts and ideas which will eventually broaden views of cytogenetics.

Dedication List of Contributors Preface Cytogenetic Alterations in Mixed Cultures of Mammalian Somatic Cells In Vitro Hybridization of Somatic Cells In Vitro Selection Pressure in Mammalian Cell Populations Phase Cinematography Studies on the Effects of Radiation and of Some Chemicals on Cells and Chromosomes Cellular Interpretation of Aspects of the Acute Mammalian Radiation Syndrome Retention of Euploidy and Mutagenicity of Heterochromatin in Culture The Nature, Origin, and Genetic Implications of Structural Abnormalities of the Sex Chromosomes in Man Chromosome Studies in the General Population Altérations Chromosomiques dans des Cellules Cultivées en Présence d'Extraits Tissulaires Hétérospécifiques Chromosomal Studies on Human Leucocytes following Treatment with Radioactive Iodine In Vivo and In Vitro The Limited In Vitro Lifetime of Human Diploid Cell Strains Regulation of DNA Replication and Variegation-Type Position Effects Identification and Characterization of Human Chromosomes by DNA Replication Sequence Mutagenic Response of Human Somatic Cell Lines DNA Synthesis and Chromosomal Morphology of Chinese Hamster Cells Cultured in Media Containing N-Deacetyl-N-Methylcolchicine (Colcemid) Heterochromatin in the Human Male Chromosome Breakage Associated with Viruses and DNA Inhibitors Quantitation and Automation of Chromosomal Data with Special Reference to the Chromosomes of the Hamshire Pig (Sus scrofa) Author Index