Current Topics in Bioenergetics - 1st Edition - ISBN: 9780121525132, 9781483216973

Current Topics in Bioenergetics

1st Edition

Volume 13

Editors: C. P. Lee
eBook ISBN: 9781483216973
Imprint: Academic Press
Published Date: 1st February 1984
Page Count: 300
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Current Topics in Bioenergetics, Volume 13 presents the biophysical and biochemical approaches that are valuable in some systems and that may be readily be applied to others. This book discusses the function and structure relationship of energy-transducing membranes that has played an essential role in the progress and development in the field of bioenergetics.

Organized into six chapters, this volume begins with an overview of the bioenergetics processes that require a molecular description of the structure and reaction mechanisms of the individual energy-transducing catalysts. This text then provides an understanding to the methodology for initiation of an electron paramagnetic resonance (EPR) investigation in this field of research. This book discusses as well the developments in spin label EPR techniques. The final chapter deals with immunological techniques that are used for studying the biochemistry and molecular biology of membrane proteins.

This book is a valuable resource for physiologists, biologists, and biochemists.

Table of Contents



Contents of Previous Volumes

Determination of the Proton Electrochemical Gradient Across Biological Membranes

I. Introduction

II. General Considerations

III. Determination of Δψ and ΔpH by Ion Distribution

IV. Determination of Δψ and ΔpH with Other Methods

V. Utilization of ΔμH Measurements



Application of Electron Paramagnetic Resonance in the Study of Iron-Sulfur Clusters in Energy-Transducing Membranes

I. Introduction

II. The EPR Properties of Fe-S Clusters

III. Characterization of EPR Signals in Energy-Transducing Membranes

IV. Quantification of Signals

V. Kinetic Measurements

VI. Orientation of Fe-S Clusters in Membranes

VII. Concluding Remarks


Recent Developments in Spin Label EPR Methodology for Biomembrane Studies

I. Introduction

II. Nitroxide Spin Labels

III. Conventional EPR

IV. Saturation Transfer EPR

V. Pulse (Time Domain) EPR

VI. Prospects for Future Applications


Probing Structure and Motion of the Mitochondrial Cytochromes

I. Introduction

II. An Overview of Mitochondrial Components

III. Complex III: Cytochrome bC1

IV. Cytochrome c

V. Complex IV or Cytochrome c Oxidase

VI. ADP/ATP Translocator

VII. Conclusions and Further Questions


Enzyme-Catalyzed Oxygen Exchange Reactions and Their Implications for Energy Coupling

I. Introduction

II. Basic Concepts and Terminology

III. Enzyme-Catalyzed Oxygen Exchanges

IV. Epilogue


Use of Immunological Techniques to Study Membrane Proteins

I. Introduction

II. Preparing Antibodies against Membrane Proteins

III. Applications of Antibodies to the Study of Membrane Proteins

IV. Conclusions




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© Academic Press 1984
Academic Press
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About the Editor

C. P. Lee

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