Cryo-EM Part B: 3-D Reconstruction

Cryo-EM Part B: 3-D Reconstruction

1st Edition - September 30, 2010

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  • Editor: Grant Jensen
  • eBook ISBN: 9780123849922
  • Hardcover ISBN: 9780123849915

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This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

Key Features

  • Includes time-tested core methods and new innovations applicable to any researcher
  • Methods included are useful to both established researchers and newcomers to the field
  • Relevant background and reference information given for procedures can be used as a guide


Biochemists, structural biologists, molecular biologists, cell biologists, and microbiologists

Table of Contents

  • 1. Fundamentals of three-dimensional reconstruction from projections
    Pawel A. Penczek
    2. Image Restoration in Cryo-electron Microscopy
    Pawel A. Penczek
    3. Resolution measures in molecular electron microscopy
    Pawel A. Penczek
    4. 3D reconstruction from 2D crystal image and diffraction data
    Andreas Schenk, Daniel Castaño-Díez, Bryant Gipson, Marcel Arheit, Xiangyan Zeng, and Henning Stahlberg
    5. Fourier-Bessel Reconstruction of Helical Assemblies
    David L Stokes, Ruben Diaz & William, J. Rice
    6. Reconstruction of Helical Filaments and Tubes
    Edward H Egelman
    7. Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage
    Jinghua Tang, Robert S. Sinkovits, and Timothy S. Baker
    8. Single Particle Analysis at High Resolution
    Yao Cong and Steven J. Ludtke
    9. The Orthogonal Tilt Reconstruction method
    Andres Leschziner
    10. An introduction to maximum-likelihood methods in cryo-EM
    Fred J. Sigworth, Peter C. Doerschuk, Jose-Maria Carazo, and Sjors H.W. Scheres
    11. Classification of structural heterogeneity by maximum-likelihood methods
    Sjors H.W. Scheres
    12. Methods for three-dimensional reconstruction of heterogeneous assemblies
    Elena V Orlova, Helen R. Saibil
    13. Alignment of cryo-electron tomography datasets
    Fernando Amat, Daniel Casta˜no-Diez, Albert Lawrence, Farshid Moussavi, Hanspeter Winkler, & Mark Horowitz
    14. Correcting for the Ewald sphere in high resolution single particle reconstructions
    Grant J Jensen, Peter A. Leong, Xuekui Yu, & Z. Hong Zhou
    15. Software Tools For Molecular Microscopy: An Open-Text Wikibook
    Bridget Carragher, Neil R. Voss, Clinton S. Potter, & Ross Smith

Product details

  • No. of pages: 456
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: September 30, 2010
  • Imprint: Academic Press
  • eBook ISBN: 9780123849922
  • Hardcover ISBN: 9780123849915

About the Serial Volume Editor

Grant Jensen

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