Description

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

Key Features

  • Covers many areas of CLEM by the best international scientists in the field
  • Includes a brief history and overview on CLEM methods
  • Readership

    Researchers and students in cell, molecular and developmental biology

    Table of Contents

    1. Imaging Fluorescently Labelled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy: Practical Considerations
    2. K. Kobayashi, D. Cheng, M. Huynh, K.R. Ratinac, P. Thordarson & F. Braet

    3. Visualising Live Dynamics and Ultrastructure of Intracellular Organelles with Pre-Embedding Correlative Light-Electron Microscopy.
    4. Roman S. Polishchuk, Elena V. Polishchuk, and Alberto Luini

    5. Correlative Fluorescence and Transmission Electron Microscopy in Tissues
    6. Toshihiro Takizawa and John M. Robinson

    7. Correlative Light and Electron Microscopy in Parasite Research
    8. Céline Loussert, Claire-Lise Forestier and Bruno M. Humbel

    9. Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy
    10. Gunar Fabig, Susanne Kretschmar, Susanne Weiche, Dominic Eberle, Marius Ader, and Thomas Kurth

    11. 3D HDO-CLEM: Cellular Compartment Analysis by Correlative Light-Electron Microscopy on Cryosection
    12. Katia Cortese, Giuseppe Vicidomini, MariaCristina Gagliani1, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti

    13. Correlative Light and Electron Microscopy of GFP
    14. Markus Grabenbauer

    15. Picking Faces Out of a Crowd: Genetic Labels for Identification of Proteins in Correlated Light and Electron Microscopy Imaging
    16. Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu and Gina E. Sosinsky

    17. Correlated Light Microscopy and Electron Microscopy (CLEM): Search… and Find!
    18. Klaas A. Sjollema, Ulrike Schnell, Jeroen Kuipers, Ruby Kalicharan & Ben N. G. Giepmans

    19. Capturing Endocytic Segregation Events with HPF-CLEM
    20. Edward Brow

    Details

    No. of pages:
    460
    Language:
    English
    Copyright:
    © 2012
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780124160262
    Electronic ISBN:
    9780123914385