Description

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.

Key Features

  • Covers many areas of CLEM by the best international scientists in the field
  • Includes a brief history and overview on CLEM methods
  • Readership

    Researchers and students in cell, molecular and developmental biology

    Table of Contents

    Series Editors

    Front Matter

    Contributors

    Preface

    Introduction to Correlative Light and Electron Microscopy

    Chapter 1 Imaging Fluorescently Labeled Complexes by Means of Multidimensional Correlative Light and Transmission Electron Microscopy

    I Introduction

    II Rationale

    III Methods

    IV Instrumentation and Materials

    V Discussion

    Chapter 2 Visualizing Live Dynamics and Ultrastructure of Intracellular Organelles with Preembedding Correlative Light-Electron Microscopy

    I Introduction and Rationale

    II Materials

    III Methods

    IV Discussion

    V Summary

    Chapter 3 Correlative Fluorescence and Transmission Electron Microscopy in Tissues

    I Introduction

    II Correlative Microscopy: Reporter Systems

    III Correlative Microscopy of Tissues

    IV Conclusions

    Chapter 4 Correlative Light and Electron Microscopy in Parasite Research

    I Introduction

    II Rationale

    III Methods

    IV Experiments and Materials

    V Results and Discussion

    VI Summary

    Chapter 5 Labeling of Ultrathin Resin Sections for Correlative Light and Electron Microscopy

    I Introduction

    II Rationale

    III Methods

    IV Materials

    V Summary and Outlook

    Chapter 6 3D HDO-CLEM

    I Introduction

    II Materials

    III Methods

    IV Notes

    Chapter 7 Correlative Light and Electron Microscopy of GFP

    I Introduction

    II Rationale

    III Methods

    IV Materials

    V Discussion

    Chapter 8 Picking Faces out of a Crowd

    I Introduction

    II The Crowded Cell and Spatiotemporal Proteomics

    III What EM has to Offer

    IV Immunomarkers

    V Genetically Appended or Inserted Protein Tags

    VI Types of Genetic Tags Currently Available

    VII Future Directions and Challenges

    Chapter

    Details

    No. of pages:
    460
    Language:
    English
    Copyright:
    © 2012
    Published:
    Imprint:
    Academic Press
    Print ISBN:
    9780124160262
    Electronic ISBN:
    9780123914385