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1. Workflow (dis)-advantages / spiderweb
2. Serial section LM + EM
Erin M. Tranfield
3. Platinum clusters as CLEM probes
4. Correlative Light Electron Microscopy with a transition metal complex as a single probe
5. Detection EDX + EELS
Ben N. G. Giepmans
8. A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes
9. Three-dimensional on-section correlative light and electron microscopy of large cellular volumes using STEM tomography
10. Accelerated procedure for approaching and imaging of optically branded ROI in tissue
Natalia V. Gounko
12. Cryo SOFI CLEM
13. Cryo fluo after Cryo lamella
14. Super resolution CLEM
15. FIB-SEM + software
16. Correlia, CLEM software
17. Correlated Multimodality Imaging beyond CLEM: From in-vivo Preclinical Imaging to ex-vivo Microscopy
18. Community efforts
Correlative Light and Electron Microscopy IV, Volume 162, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Besides the detailed description of protocols for CLEM technologies including time-resolution, Super resolution LM and Volume EM, new chapters cover Workflow (dis)-advantages/spiderweb, Serial section LM + EM, Platinum clusters as CLEM probes, Correlative Light Electron Microscopy with a transition metal complex as a single probe, SEM-TEM-SIMS, HPF-CLEM, A new workflow for high-throughput screening of mitotic mammalian cells for electron microscopy using classic histological dyes, and more.
- Contains contributions from experts in the field
- Covers topics using nano-SIMS and EDX for CLEM
- Presents recent advances and currently applied correlative approaches
- Gives detailed protocols, allowing for the application of workflows in one’s own laboratory setting
- Covers CLEM approaches in the context of specific applications
- Aims to stimulate the use of new combinations of imaging modalities
PhD students, Post-doctoral researchers, and imaging facility staff working in the life science research area with an interest in microscopy technologies and applying or aiming to apply Correlative Microscopy techniques to their research
- No. of pages:
- © Academic Press 2021
- 1st March 2021
- Academic Press
- Hardcover ISBN:
Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM. TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019. He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy.
Professor of Structural Cell Biology, Technische Universitat Dresden, TU Dresden, Germany
Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. His focus is on the study of sorting mechanisms in intracellular transport pathways and in the area of Synthetic Biology. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 85 papers and edited 4 books on CLEM (including 3 Volumes of the Methods in Cell Biology series). PV obtained his PhD on electron microscopic studies of the peripheral nervous system at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He has acted as chair and co-chair of the Electron Microscopy section of the Royal Microscopical Society and is closely involved with BioimagingUK shaping the UK imaging infrastructure landscape. He has organised and taught on several courses and workshops on subjects such as high-pressure freezing, Correlative Light Electron Microscopy, and immuno EM. His lab is the home of the EMBO practical course on CLEM and he is Work Group leader for CLEM within the EU COST project COMULIS (COrrelated MUltimodal imaging in LIfe Sciences).
Professor of Bioimaging, University of Bristol, UK
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