Comprehensive Biophysics

1st Edition

Editor-in-Chiefs: Edward Egelman
Hardcover ISBN: 9780123749208
eBook ISBN: 9780080957180
Imprint: Academic Press
Published Date: 11th April 2012
Page Count: 3524

Institutional Access


Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics will help bridge that communication gap.

Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge.

Key Features

  • Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding
  • The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research
  • Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource


Researchers, advanced undergraduate and graduate students, postdoctoral fellows, senior investigators, corporate customers (such as biotech companies) and libraries from Physics, Chemistry and Life Sciences departments

Table of Contents

Editorial Board


Permission Acknowledgments

Volume 1 Biophysical Techniques for Structural Characterization of Macromolecules

1.1 Volume Introduction

Overview and Historical

1.2 Cantor and Schimmel – 30 Years Later

Protein Production Strategies for High-Throughput Structure Determination

1.3 Efficient Strategies for Production of Eukaryotic Proteins


1.3.1 Introduction

1.3.2 Selection of Expression Platform

1.3.3 Cloning

1.3.4 Expression and Recovery of Target Protein

1.3.5 Facility

1.3.6 Rescue and Optimization Strategies

1.3.7 Conclusion



X-ray Crystallography

1.4 X-Ray Crystallography: Crystallization


1.4.1 Introduction

1.4.2 Structure Determination Process Overview

1.4.3 Precrystallization Studies

1.4.4 Crystallization Process

1.4.5 High-Throughput Crystallization


1.5 X-Ray Crystallography: Data Collection Strategies and Resources


1.5.1 Basic Concepts in X-Ray Crystal Diffraction

1.5.2 X-Ray Sources and Instrumentation

1.5.3 Data Collection Strategies


1.6 Phasing of X-ray Data

1.6.1 The Phase Problem or What Can Go Wrong?

1.6.2 Direct Methods: What We Know About the Unmeasured Phases?

1.6.3 The Patterson Function: Who Needs the Phases?

1.6.4 Dual-Space Recycling: Enforcing Atomicity in Real and Reciprocal Space

1.6.5 Experimental Phasing: Divide and Conquer

1.6.6 Density Modification: Improving Experimental Phases

1.6.7 Molecular Replacement: Exploiting Previous Structural Information

1.6.8 Frontier Methods: Not General – Yet?

1.6.9 Multiple Beam Interference: But It Is Possible to Measure Phases

1.6.10 Multisolution Combination of Lo


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About the Editor-in-Chief

Edward Egelman

Edward Egelman received a BA in physics and a PhD in biophysics from Brandeis University. He was a postdoctoral fellow at the Medical Research Council Laboratory of Molecular Biology in Cambridge, UK, and then an assistant professor at Yale University. He was an associate and full professor at the University of Minnesota Medical School, and then moved to the University of Virginia in 1999 where he is a professor of biochemistry and molecular genetics. He is currently editor-in-chief of Biophysical Journal. He has been elected a fellow of the Biophysical Society and of the American Academy of Microbiology. His research focuses on the structure and function of macromolecular complexes, mainly using electron cryo-microscopy and computational image analysis. He has spent many years studying the structure of F-actin, as well as helical nucleoprotein complexes formed by recombination proteins (such as the bacterial RecA and the eukaryotic Rad51) on DNA.

Affiliations and Expertise

Editor-in-chief of Biophysical Journal. Elected fellow of the Biophysical Society and of the American Academy of Microbiology.