Column Handbook for Size Exclusion Chromatography - 1st Edition - ISBN: 9780127655550, 9780080529394

Column Handbook for Size Exclusion Chromatography

1st Edition

Editors: Chi-san Wu
eBook ISBN: 9780080529394
Imprint: Academic Press
Published Date: 12th March 1999
Page Count: 637
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Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography.

Key Features

@introbul:Key Features @bul:* Evaluate and select columns with confidence for specific applications

  • Optimize separations and improve the ruggedness of analytical methods
  • Extend the service time of a column
  • Establish a quality-control program to ensure consistency in column performance
  • Avoid the expense of column damage or purchases that do not give the expected results


Analytical, bio, polymer, physical, and organic chemists; quality assurance managers; graduate students.

Table of Contents

Contribution from SEC and GFC Column Manufacturers: M. Lu, The Preparation of Beaded Organic Polymers and Their Application in Size Exclusion Chromatography. A. Williams and L. Hagel, Size Exclusion for Analysis and Purification of Aqueous Macromolecules. R. Moody, Zorbax Porous Silica Microsphere Columns for High Performance Size Exclusion Chromatography. Y. Kato, K. O'Donnel, and J. Fisher, Size Exclusion Chromatography Using TSK GEL Columns and Toyopearl Resins. J.E. Armonas and B.H. Peabody, American Polymer Standards GPC/SEC Columns. H. Suzuki and S. Denko, Shodex Columns for Size Exclusion Chromatography. L.R. Jacob and L. Britsch, Size Exclusion Chromatography on Fractogel EMD BioSEC. A. Alpert, Size Exclusion HPLC of Small Solutes. P. Kilz, Design, Properties, and Testing of SEC Columns and Optimization of SEC Separations. K. Gooding, SynChropak Size Exclusion Columns. U. Neue, Waters Columns for Size Exclusion Chromatography. E. Meehan, Size Exclusion Chromatography Columns from Polymer Laboratories. H. Jordi, Jordi Gel Columns for Size Exclusion Chromatography. Contribution from SEC and GFC Column Users: R. Bruessau, General Characterization of GPC Columns. D. Berek, Interactive Properties of Polystyrene/Divinylbenzene Based Commercial SEC Columns. A. Huber and W. Praznik, Analytical and Preparative Columns for Aqueous SEC of Polysaccharides. C. Wu, L. Senak, D. Osborne, and T. Cheng, Comparison of Four Commercial Linear Aqueous Size Exclusion Columns and Four Sets of Commercial Polyethylene Oxide Standards for Aqueous Size Exclusion Chromatography of Polyvinylpyrrilodone and Polyethylene Oxide. D. Allen, Application of Size Exclusion-High Performance Liquid Chromatography for Biopharmaceutical Proteins and Peptide Therapeutics. M. Bender and D. Saucy, Column Selection and Related Issues for Acrylic Acid and Acrylic Ester Polymers. D. Nagy, Application and Uses of Columns for Aqueous SEC of Water Soluble Polymers. A.W. deGroot, Quality Control of Columns for High Temperature. Columns for Other Related Polymer Separation or Fractionation Techniques: S.S. Huang, Molecular Weight Separations of Macromolecules by Hydrodynamic Chromatography. I. Teraoka, Columns for High Osmotic Pressure Chromatography. Index.


No. of pages:
© Academic Press 1999
Academic Press
eBook ISBN:

About the Editor

Chi-san Wu

Affiliations and Expertise

International Specialty Products


@qu:"This book is definitely a valuable resource for researchers working in the area of polymer science and analytical chemistry. The book is likely to be highly cited in the future and is therefore highly recommended. This book should find a respectable place in most libraries." @source:--POLYMER NEWS, Vol. 25, No. 1 @qu:"The book is well produced with a consistent style and a comprehensive index. It clearly belongs on the laboratory shelf of every analyst routinely using SEC." @source:--ROYAL SOCIETY OF CHEMISTRY

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