Cilia and Flagella, Volume 47

1st Edition

Serial Editors: Leslie Wilson Paul Matsudaira
Serial Volume Editors: William Dentler George Witman
Hardcover ISBN: 9780125641487
eBook ISBN: 9780080859453
Imprint: Academic Press
Published Date: 27th July 1995
Page Count: 603
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Table of Contents

Isolation of Cilia and Flagella:

P.A. Lefebvre, Flagellar Amputation and Regeneration in Chlamydomonas.

S.M. King, Large-Scale Isolation of Chlamydomonas Flagella.

W.L. Dentler, Isolation of Cilia from Tetrahymena thermophila.

D.L. Nelson, Preparation of Cilia and Subciliary Fractions from Paramecium.

H.M. Ngo and G.B. Bouck, Isolation of Euglena Flagella.

J.T.S. Agustin and G.B.Witman, Isolation of Ram Sperm Flagella.

R.E. Stephens, Isolation of Molluscan Gill Cilia, Sperm Flagella, and Axonemes.

L.H. Cheung, Isolation of Flagella and Their Membranes from Sea Urchin Spermatozoa.

C. Saudrais and J.-L. Gatti, Isolation of Fish Sperm Flagella.

Culture and Isolation of Ciliated Epithelial Cells and Cilia:

L.E. Ostrowski, S.H. Randell, A.B. Clark, T.E. Gray, and P. Nettesheim, Ciliogenesis of Rat Tracheal Epithelial Cells in Vitro.

E.R. Dirksen, J.A. Felix, and M.J. Sanderson, Preparation of Explant and Organ Cultures and Single Cells from Airway Epithelium.

B. Chailley and E. Boisvieux-Ulrich, Induction of Ciliogenesis in Oviduct Epithelium.

K. Pagh-Roehl and B. Burnside, Preparation of Teleost Rod Inner and Outer Segments.

A.T. Hastie, Isolation of Respiratory Cilia.

General Methods:

G. Piperno, Electrophoretic Separation of Dynein Heavy Chains.

G. Piperno, Two-Dimensional Separation of Axonemal Proteins.

T. Otter, Analysis of Flagellar Calcium-Binding Proteins.

R.A. Bloodgood and N.L. Salomonsky, Phosphorylation of Chlamydomonas Flagellar Pr


Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls of the procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of "classic" and innovative techniques essential to the study of cilia and flagella.

Key Features

@introbul:Key Features @bul:* A complete guide containing more than 80 concise technical chapters friendly to both the novice and experienced researcher

  • Covers protocols for cilia and flagella across systems and species from Chlamydomonas and Euglena to mammals
  • Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time, including microscopy, electrophoresis, and PCR
  • Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures


Graduate students, technicians, clinicians, and experienced researchers interested in the study of cilia and flagella. Cell, developmental, and molecular biologists, biochemists, geneticists, physiologists, neurobiologists, medical researchers, and biotechnologists.


No. of pages:
© Academic Press 1995
Academic Press
eBook ISBN:
Hardcover ISBN:

About the Serial Editors

Leslie Wilson Serial Editor

Affiliations and Expertise

University of California, Santa Barbara, U.S.A.

Paul Matsudaira Serial Editor

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

About the Serial Volume Editors

William Dentler Serial Volume Editor

Affiliations and Expertise

University of Kansas, Lawrence, U.S.A.

George Witman Serial Volume Editor

Affiliations and Expertise

Department of Cell Biology, University of Massachusetts Medical School, USA