Cilia and Flagella presents protocols accessible to all individuals working with eukaryotic cilia and flagella. These recipes delineate laboratory methods and reagents, as well as critical steps and pitfalls of the procedures. The volume covers the roles of cilia and flagella in cell assembly and motility, the cell cycle, cell-cell recognition and other sensory functions, as well as human diseases and disorders. Students, researchers, professors, and clinicians should find the book's combination of "classic" and innovative techniques essential to the study of cilia and flagella.

Key Features

@introbul:Key Features @bul:* A complete guide containing more than 80 concise technical chapters friendly to both the novice and experienced researcher * Covers protocols for cilia and flagella across systems and species from Chlamydomonas and Euglena to mammals * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time, including microscopy, electrophoresis, and PCR * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes, who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures


Graduate students, technicians, clinicians, and experienced researchers interested in the study of cilia and flagella. Cell, developmental, and molecular biologists, biochemists, geneticists, physiologists, neurobiologists, medical researchers, and biotechnologists.

Table of Contents

Isolation of Cilia and Flagella: P.A. Lefebvre, Flagellar Amputation and Regeneration in Chlamydomonas. S.M. King, Large-Scale Isolation of Chlamydomonas Flagella. W.L. Dentler, Isolation of Cilia from Tetrahymena thermophila. D.L. Nelson, Preparation of Cilia and Subciliary Fractions from Paramecium. H.M. Ngo and G.B. Bouck, Isolation of Euglena Flagella. J.T.S. Agustin and G.B.Witman, Isolation of Ram Sperm Flagella. R.E. Stephens, Isolation of Molluscan Gill Cilia, Sperm Flagella, and Axonemes. L.H. Cheung, Isolation of Flagella and Their Membranes from Sea Urchin Spermatozoa. C. Saudrais and J.-L. Gatti, Isolation of Fish Sperm Flagella. Culture and Isolation of Ciliated Epithelial Cells and Cilia: L.E. Ostrowski, S.H. Randell, A.B. Clark, T.E. Gray, and P. Nettesheim, Ciliogenesis of Rat Tracheal Epithelial Cells in Vitro. E.R. Dirksen, J.A. Felix, and M.J. Sanderson, Preparation of Explant and Organ Cultures and Single Cells from Airway Epithelium. B. Chailley and E. Boisvieux-Ulrich, Induction of Ciliogenesis in Oviduct Epithelium. K. Pagh-Roehl and B. Burnside, Preparation of Teleost Rod Inner and Outer Segments. A.T. Hastie, Isolation of Respiratory Cilia. General Methods: G. Piperno, Electrophoretic Separation of Dynein Heavy Chains. G. Piperno, Two-Dimensional Separation of Axonemal Proteins. T. Otter, Analysis of Flagellar Calcium-Binding Proteins. R.A. Bloodgood and N.L. Salomonsky, Phosphorylation of Chlamydomonas Flagellar Pr


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© 1995
Academic Press
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