3rd Edition - January 1, 1969

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  • Editor: Ivor Smith
  • eBook ISBN: 9781483281322

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Chromatographic and Electrophoretic Techniques, Volume I — Chromatography focuses on techniques, processes, reactions, and methodologies involved in chromatography. The selection first ponders on paper chromatographic apparatus and techniques; desalting and related techniques; and apparatus and techniques in thin layer chromatography. Discussions focus on chromatographic solvents, location reagents, chemical conversions occurring during electrolytic desalting, electrodialysis, and ion exchange desalting. The book also examines paper chromatography, applications of thin layer chromatography in clinical biochemistry, and dinitro-phenyl aminoacids. The publication takes a look at iodoaminoacids and related compounds, indoles and related Ehrlich reactors, and imidazoles. The book also elaborates on guanidines, purines and pyrimidines and their derivatives, sugars, ketoacids, organic and phenolic acids, and chromatographic procedures. The selection is a dependable reference for biochemists and readers interested in chromatography.

Table of Contents

  • 1. Introduction to Paper and Thin Layer Chromatography

    2. Paper Chromatographic Apparatus and Techniques

    3. Desalting and Related Techniques

    4. Thin Layer Chromatography (TLC)

    Section 1 — Apparatus and Techniques

    Section 2 — Pre-Coated Plates

    Section 3 — Non-Adhering Layers

    5. Aminoacids, Amines and Related Compounds

    The Pathological Aminoacidurias

    6. Dinitro-Phenyl Aminoacids

    7. Aminoacid Phenylthiohydantoins, Dimethylaminonaphthalenesulphonyl Aminoacids and Sequence Analysis of Peptides and Proteins

    8. Iodoaminoacids and Related Compounds

    9. Indoles and Related Ehrlich Reactors

    10. Imidazoles

    11. Guanidines

    12. Purines, Pyrimidines and Their Derivatives

    13. Sugars and Related Compounds

    14. Ketoacids

    15. Organic Acids of the Citric Acid Cycle and Related Acids

    16. Phenolic Acids

    17. Plant Phenols and Tannins

    18. Lipids

    19. Separation and Estimation of Phospholipids

    20. Barbiturates

    21. Alkaloids

    22. Glutarimides and Other Neutral Drugs

    23. Steroids

    24. Rm and λRm: Making Full Use of Rf Values

    25. Chromatography of Radioactive Substances

    26.Chromatography of Labeled Cell Extracts

    27. The Spark Chamber Technique for Radio-Chromatograms

    28. Catecholamlnes, Their Precursors and Metabolites

    29. Ion-Exchange Celluloses in Chromatography

    30. Amino Sugars

    31. Kynurenine Pathway Metabolites of Tryptophan

    32. Inorganic Ions

    33. Sephadex Thin-Layer Gel Filtration

    34. Preparative Layer Chromatography

    35. Automated Quantitative Analysis of Paper Chromatogram Strips

    36. Pigments

    The Forensic Examination of Inks

    pH Indicators, Anthocyanins and Pteridines

    37. Column Chromatographic Apparatus and Techniques

    38. The Investigation of New Compounds

    39. Fractionation and Sequence Analysis of [32p]. Labeled Ribonucleotides

    40. Modelsformacromolecules, Nucleic Acids and Proteins

    General Index

Product details

  • No. of pages: 1100
  • Language: English
  • Copyright: © Butterworth-Heinemann 1969
  • Published: January 1, 1969
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483281322

About the Editor

Ivor Smith

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