Description

DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the chromatin remodelling enzymes play an important role in gene transcription.

Key Features

*Genetic assays of chromatin modification and remodeling *Histone modifying enzymes *ATP-dependent chromatin remodeling enzymes

Readership

Biochemists, cell biologists, molecular biologists, geneticists, biophysicists

Table of Contents

Functional Analyses of Chromatin Modifications in Yeast; Genetic Analysis of Chromatin REmodeling Using Budding Yeast as a Model; Introduction to Trx-G and Pc-G Genes; Genetic and Cytological Analysis of Chromatin-Remodeling Factors; Genetics Analysis of h1 Linker Histone Subtypes and Their Functions in Mice; The Use of Mass Spectrometry for the Analysis of Histone Modifications; Histone Modification Patterns During Gene Activation; Identification and Analysis of Native HAT Complexes; Histone Deacetylases: Purification of the Enzymes, Substrates, and Assay Conditions; Measurement of Mammalian Histone Deacetylase Activity; Analysis of Histone Phosphorylation: Coupling Intracellular Signaling to Chromatin Remodeling; Purification of Histone Methyltransferases from HeLa Cells; Global Proteomic Analysis of S. cerevisiae (GPS) to Identify Proteins Required for Histone Modifications; Purification of Sir2 Proteins from Yeast; Purification and Biochemical Properties of the Drosophila TAC1 Complex; Isolation and Chracterization of CHRASCH, a Polycomb-Cotaining Silencing Complex; Biochemical Analysis of Mammalian Polycomb Group Protein Complexes and the Identification of GEnetic Elements that Block Polycomb-Mediated Gene Repression; Purification and Functional Analysis of the Mammalian SWI/SNF-Family of Chromatin-Remodeling Complexes; Isolation and Assay of the RSC Chromatin-Remodeling Complex from Saccharomyces cerevisiae; DNA Translocation and Nucleosome Remodeling Assays by the RSC Chromatin Remodeling Complex; A Nucleosome Sliding Assay for Chromatin Remodeling Factors; Methods for Analysis of Nucleosomes Sliding by Drosophila NURF; Methods for Preparation and Assays for Xenopus ISWI Complexes; Functional Analysis of ISWI Complexes in Mammalian Cells; Preparation and Assays for Mammalian ISWI Complexes; Preparation and Analysis of the INO80 Complex; Assay of Z-DNA Induction by Chromatin Remodeling Factors; Immuno-Depletion and

Details

No. of pages:
546
Language:
English
Copyright:
© 2004
Published:
Imprint:
Academic Press
eBook ISBN:
9780080497150
Print ISBN:
9780121827816
Print ISBN:
9780124112919

About the authors

Carl Wu

Affiliations and Expertise

National Cancer Institute, Bethesda, MD

C. Allis

Affiliations and Expertise

The Rockefeller University, New York, NY