Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

1st Edition - May 29, 2020
This is the Latest Edition
  • Editor: David M. Chenoweth
  • Hardcover ISBN: 9780128211519
  • eBook ISBN: 9780128211526

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Description

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

      1. Fluorogenic detection of protein aggregates in live cells using the AggTag method
      2. Kwan Ho Jung and Xin Zhang

      3. Synthesis and application of a ratiometric probe for hydrogen peroxide
      4. Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains

      5. Protein labeling for FRET with methoxycoumarin and acridonylalanine
      6. Chloe M. Jones, Yarra Venkatesh and E. James Petersson

      7. Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays
      8. Paul T. Ludford III and Yitzhak Tor

      9. Method to discriminate amyloids using fluorescent probes
      10. Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang

      11. Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives
      12. Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas

      13. Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer
      14. John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo

      15. Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles
      16. Nanxi Wang and Lei Wang

      17. 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy
      18. Arusha Acharyya, Ismail A. Ahmed and Feng Gai

      19. Dissecting contributions of catalytic and reader domains in regulation of histone demethylation
      20. Nektaria Petronikolou, James E. Longbotham and Danica Galonić Fujimori

      21. Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization
      22. Qing Zhang, Yue Zhang and Xuechen Li

      23. Chemical synthesis of di-ubiquitin modified histones for further biochemical studies
      24. Jia-Bin Li, Jun Liang and Changlin Tian

      25. Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies
      26. Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis

      27. FlICk (fluorescent isoindole crosslinking) for peptide stapling
      28. Mihajlo Todorovic and David M. Perrin

      29. Selective protein N-terminal labeling with N-hydroxysuccinimide esters
      30. Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey

      31. Covalent live-cell labeling of proteins using a photoreactive fluorogen
      32. Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra

      33. Photoconvertible diazaxanthilidene dyes for live cell imaging
      34. Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth

      35. No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag

    Wei Sheng, James H. Geiger and Babak Borhan

Product details

  • No. of pages: 430
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: May 29, 2020
  • Imprint: Academic Press
  • Hardcover ISBN: 9780128211519
  • eBook ISBN: 9780128211526
  • About the Serial Volume Editor

    David M. Chenoweth

    David Chenoweth is at University of Pennsylvania, PA, USA

    Affiliations and Expertise

    University of Pennsylvania, PA, USA