Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods

1st Edition - July 23, 2020

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  • Editor: David M. Chenoweth
  • eBook ISBN: 9780128211557
  • Hardcover ISBN: 9780128211564

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Description

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing β-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

      1. Enzyme- or light-triggered cyclopropenes for bioorthogonal ligation
      2. Ting Jiang and Scott T. Laughlin

      3. Analysis of metabolically labeled inositol phosphate messengers by NMR
      4. Robert Puschmann, Robert K. Harmel and Dorothea Fiedler

      5. Photo-releasable derivatives of inositol pyrophosphates
      6. Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz

      7. IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation
      8. Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin

      9. Fluorescent bioorthogonal labeling of class B GPCRs in live cells
      10. Srikanth Kumar Gangam and Qing Lin

      11. Near-infrared photoactivatable nitric oxide donors with photoacoustic readout
      12. Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan

      13. H2S donors with optical responses
      14. Michael D. Pluth, Yu Zhao and Matthew M. Cerda

      15. Visualization of oxidative stress-induced carbonylation in live mammalian cells
      16. Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane

      17. Receptor-based fluorescent sensors constructed from ribonucleopeptide
      18. Skun Nakano, Hiroaki Konishi and Takashi Morii

      19. Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy
      20. Siddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann

      21. A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore
      22. Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann

      23. Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay
      24. Kirsten Deprey and Joshua A. Kritzer

      25. Cell-penetrating and mitochondrion-targeting molecules
      26. George Appiah Kubi and Dehua Pei

      27. A photocaged DNA nanocapsule for delivery and manipulation in cells
      28. Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo

      29. A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
      30. Esther Braselmann and Amy E. Palmer

      31. Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)
      32. Kayla N. Busby and Neal K. Devaraj

      33. Assaying RNA solvent accessibility in living cells with LASER
      34. Chao Feng and Robert C. Spitale

      35. Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics
      36. Jeffrey A. Dewey and Bryan C. Dickinson

      37. Cross-linking cellular nucleic acids via a target-directing double click reagent
      38. Masayuki Tera and Nathan W. Luedtke

      39. Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes
      40. Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard

      41. Quantification of protein delivery in live cells using fluorescence correlation spectroscopy

    Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz

Product details

  • No. of pages: 526
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: July 23, 2020
  • Imprint: Academic Press
  • eBook ISBN: 9780128211557
  • Hardcover ISBN: 9780128211564

About the Serial Volume Editor

David M. Chenoweth

David Chenoweth is at University of Pennsylvania, PA, USA

Affiliations and Expertise

University of Pennsylvania, PA, USA

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