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Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods - 1st Edition - ISBN: 9780128211564, 9780128211557

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641

1st Edition

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Serial Volume Editor: David M. Chenoweth
Hardcover ISBN: 9780128211564
eBook ISBN: 9780128211557
Imprint: Academic Press
Published Date: 23rd July 2020
Page Count: 526
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Table of Contents

    1. Enzyme- or light-triggered cyclopropenes for bioorthogonal ligation
    2. Ting Jiang and Scott T. Laughlin

    3. Analysis of metabolically labeled inositol phosphate messengers by NMR
    4. Robert Puschmann, Robert K. Harmel and Dorothea Fiedler

    5. Photo-releasable derivatives of inositol pyrophosphates
    6. Sebastian Hauke, Tamara Bittner, Henning J. Jessen and Carsten Schultz

    7. IMPACT: Imaging phospholipase D activity with clickable alcohols via transphosphatidylation
    8. Timothy W. Bumpus, Dongjun Liang and Jeremy M. Baskin

    9. Fluorescent bioorthogonal labeling of class B GPCRs in live cells
    10. Srikanth Kumar Gangam and Qing Lin

    11. Near-infrared photoactivatable nitric oxide donors with photoacoustic readout
    12. Effie Y. Zhou, Hailey J. Knox, Christopher J. Reinhardt, Gina Partipilo and Jefferson Chan

    13. H2S donors with optical responses
    14. Michael D. Pluth, Yu Zhao and Matthew M. Cerda

    15. Visualization of oxidative stress-induced carbonylation in live mammalian cells
    16. Kamalika Mukherjee, Tak Ian Chio and Susan L. Bane

    17. Receptor-based fluorescent sensors constructed from ribonucleopeptide
    18. Skun Nakano, Hiroaki Konishi and Takashi Morii

    19. Conformationally restrained pentamethine cyanines and use in reductive single molecule localization microscopy
    20. Siddharth S. Matikonda, Ralph Götz, Ryan McLaughlin, Markus Sauer and Martin J. Schnermann

    21. A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore
    22. Michael P. Luciano, Saghar Nourian, Alexander P. Gorka, Roger R. Nani, Tadanobu Nagaya, Hisataka Kobayashi and Martin J. Schnermann

    23. Quantitative measurement of cytosolic penetration using the chloroalkane penetration assay
    24. Kirsten Deprey and Joshua A. Kritzer

    25. Cell-penetrating and mitochondrion-targeting molecules
    26. George Appiah Kubi and Dehua Pei

    27. A photocaged DNA nanocapsule for delivery and manipulation in cells
    28. Yihong Feng, Takeshi Tohgasaki, Yasuyuki Shitomi, Hiroshi Sugiyama and Masayuki Endo

    29. A multicolor riboswitch-based platform for imaging of RNA in live mammalian cells
    30. Esther Braselmann and Amy E. Palmer

    31. Enzymatic covalent labeling of RNA with RNA transglycosylation at guanosine (RNA-TAG)
    32. Kayla N. Busby and Neal K. Devaraj

    33. Assaying RNA solvent accessibility in living cells with LASER
    34. Chao Feng and Robert C. Spitale

    35. Split T7 RNA polymerase biosensors to study multiprotein interaction dynamics
    36. Jeffrey A. Dewey and Bryan C. Dickinson

    37. Cross-linking cellular nucleic acids via a target-directing double click reagent
    38. Masayuki Tera and Nathan W. Luedtke

    39. Click chemistry-based amplification and detection of endogenous RNA and DNA molecules in situ using clampFISH probes
    40. Sepideh Tavakoli, Yifang Liu, Jacob Potts and Sara H. Rouhanifard

    41. Quantification of protein delivery in live cells using fluorescence correlation spectroscopy

Susan L. Knox, Angela Steinauer, Garrett Alpha-Cobb, Adam Trexler, Elizabeth Rhoades and Alanna Schepartz


Description

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Chemical, Optical and Bioorthogonal Methods, Volume 641 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include caged cyclopropanes with improved tetrazine ligation kinetics, an analysis of metabolically labeled inositol phosphate messengers by NMR, cell-permeant caged inositol pyrophosphates for probing β-cells, imaging phospholipase D activity with clickable alcohols via transphosphatidylation, fluorescent biorthogonal labeling of class B GPCRs in live cells, near-infrared photoactivatable nitric oxide donors with integrated photoacoustic monitoring, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
526
Language:
English
Copyright:
© Academic Press 2020
Published:
23rd July 2020
Imprint:
Academic Press
Hardcover ISBN:
9780128211564
eBook ISBN:
9780128211557

Ratings and Reviews


About the Serial Volume Editor

David M. Chenoweth

David Chenoweth is at University of Pennsylvania, PA, USA

Affiliations and Expertise

University of Pennsylvania, PA, USA