Chemical Microbiology

Chemical Microbiology

1st Edition - April 1, 2022
This is the Latest Edition
  • Editor: Erin Carlson
  • Hardcover ISBN: 9780323912228

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Description

Clinical Microbiology, Volume 664 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Synthesis of chemical probes to study bacterial adenylating enzymes, Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux, Combining informatics with ABPP to identify serine hydrolyses in bacteria, A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS‐CoV‐2, Activity-based probes for bacterial histidine kinases,  Metabolomic approaches to enzyme function and pathway discovery, Identification of bile salt hydrolase activity in gut microbiota, and much more. Other chapters cover Multiplex fluorescence screening and identification using multiplex TMT, Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR, Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry, Proteomics of short-chain fatty acid probes in Salmonella, Development and application of highly sensitive labeling reagents for amino acids, and a variety of other timely topics.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology serials
  • Updated release includes the latest information on Chemical Microbiology

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Synthesis of chemical probes to study bacterial adenylating enzymes
    Courtney Aldrich
    2. Fluoroquinolone-derived fluorescent probes for studies of bacterial penetration and efflux
    Mark Blaskovich
    3. Combining informatics with ABPP to identify serine hydrolyses in bacteria
    Matthew Bogyo
    4. A Ligand Selection Strategy Identifies Chemical Probes Targeting the Proteases of SARS‐CoV‐2
    Thomas Boettcher
    5. Activity-based probes for bacterial histidine kinases
    Erin Carlson
    6. Metabolomic approaches to enzyme function and pathway discovery
    Luiz Carvalho
    7. Identification of bile salt hydrolase activity in gut microbiota
    Pamela Chang
    8. Adding a diazo-transfer reagent to culture to generate secondary metabolite probes for click chemistry
    Rachel Codd
    9. Multiplex fluorescence screening and identification using multiplex TMT
    Gideon Davies
    10. Customized Peptidoglycan Surfaces to Investigate Innate Immune Recognition via SPR
    Catherine L. Grimes and Ha Thi Thu Le
    11. Site-Specific Siderocalin Binding to Ferric and Ferric-Free Enterobactin As Revealed by Mass Spectrometry
    Michael Gross
    12. Proteomics of short-chain fatty acid probes in Salmonella
    Howard Hang
    13. Development and application of highly sensitive labeling reagents for amino acids
    Hideaki Kakeya
    14. Bacterial Cell Wall Modification with a Glycolipid Substrate
    Laura Kiessling
    15. Kinetic dissection of macromolecular complex formation with minimally-perturbing fluorescent probes
    Rahul M. Kohli
    16. Screening an Established Natural Product Library Identifies Secondary Metabolites that Potentiate
    Conventional Antibiotics
    Christian Melander
    17. Reactivity-Based Screening for Citrulline-Containing Natural Products Reveals a Family of Bacterial Peptidyl
    Arginine Deiminases
    Douglas A. Mitchell
    18. An Inactive Dispersin B Probe for Monitoring PNAG Production in Biofilm Formation
    Mark Nitz
    19. A Fluorescent Teixobactin Analogue
    James S. Nowick
    20. Mobilization of Cryptic Antibiotic Biosynthesis Loci from Human-Pathogenic Nocardia
    Sacha Pidot
    21. Remodeling of Cross-bridges Controls Peptidoglycan Cross-linking Levels in Bacterial Cell Walls
    Marcos Pires
    22. Endogenous Enzymes Enable Antimicrobial Activity
    Ronald T. Raines and Kenton Hetrick
    23. Identification of terpene synthases in higher fungi: combining analytical and bioinformatic methods
    Martin Ruehl
    24. Utilizing Imaging Mass Spectrometry to Analyze Microbial Biofilm Chemical Responses to Exogenous Compounds
    Laura M. Sanchez
    25. Finding Cryptic Metabolites with Transposon Mutagenesis and Next-Generation Mass Spectrometry
    Mohammad R. Seyedsayamdost
    26. Mechanisms of Incorporation for D‐Amino Acid Probes That Target Peptidoglycan Biosynthesis
    Mike Van Nieuwenhze
    27. Activity-Based Protein Profiling of Bile Salt Hydrolysis in the Human Gut Microbiome with Beta-Lactam or Acrylamide-Based Probes
    Aaron Wright
    28. Evolution-guided discovery of antibiotics that inhibit peptidoglycan remodelling
    Gerry D. Wright, Min Xu and Wenliang Wang
    29. Engineered Biosynthesis of Alkyne-tagged Polyketides
    Wenjun Zhang
    30. APEX peroxidase-mediated protein labeling for subcellular protein localization in mycobacteria
    Jessica Seeliger

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: April 1, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323912228
  • About the Serial Volume Editor

    Erin Carlson

    Erin E. Carlson received her B.A. at St. Olaf College (Northfield, MN) in 2000. She went on to graduate studies funded by the NIH Predoctoral Biotechnology Training Program at the University of Wisconsin - Madison and earned a Ph.D. in organic chemistry in 2005 under the direction of Professor Laura L. Kiessling. Her graduate career focused on the design and synthesis of mechanistic probes and inhibitors for carbohydrate-binding proteins, concentrating on the study of UDP-galactopyranose mutase (UGM), an enzyme involved in cell wall biosynthesis of Mycobacterium tuberculosis. Subsequently, Dr. Carlson was awarded an American Cancer Society Postdoctoral Fellowship for studies at The Scripps Research Institute with Professor Benjamin F. Cravatt. Carlson and Cravatt developed a global metabolite profiling strategy that utilizes chemoselective probes to enable enrichment and profiling of metabolites from complex biological systems. This technology, referred to as Metabolite Enrichment by Tagging and Proteolytic Release (METPR), facilitates the rigorous characterization of biochemical pathways through their most sensitive reporter, endogenous small molecules. In 2007, Dr. Carlson received an NIH Pathway to Independence Award (K99/R00) and joined the faculty at Indiana University in 2008. In the summer of 2014, she joined the faculty in the Chemistry Department at the University of Minnesota and was appointed as a Graduate Faculty member of the Department of Medicinal Chemistry, the Department of Biochemistry, Molecular Biology and Biophysics and the graduate program in Biomedical Informatics and Computational Biology in 2015. Since the start of her independent career, she has won numerous awards including being named a Presidential Early Career Awards for Scientists and Engineers (PECASE) recipient, a Pew Biomedical Scholar, the NIH Director’s New Innovator Award, the Indiana University Outstanding Junior Faculty Award, the NSF CAREER Award, the Cottrell Scholar Award and was named a Sloan Research Fellow and an Indiana University Dean's Fellow. Professor Carlson has been highlighted in several videos including one produced by NBC in their Science Behind The News series, a fast-paced video series supported by the National Science Foundation. This piece was one of five videos highlighting work funded by NSF's Directorate for Mathematical and Physical Sciences. She was also featured in a "Brilliant Minds" video. Dr. Carlson was also named one of "Tomorrow's PIs" in the sixth annual issue of Genome Technology and received the Outstanding Postdoctoral Mentor Award from the University of Minnesota Postdoctoral Association in 2017.

    Affiliations and Expertise

    Associate Professor, Department of Chemistry, University of Minnesota, USA