Cells and Tissues in Culture
Methods, Biology and Physiology
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Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology. This book focuses on the three methods of culture—tissue culture, cell culture, and organ culture.Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyroid hormone on bone are also elaborated. This volume is recommended for biologists and specialists interested in the culture of cells and tissues.
Table of Contents
Contributors to Volume 1
Foreword to Volume 1
Contents of Volume 2
Contents of Volume 3
1. Introduction
2. Methods
I. Basic Requirements
II. Tissue Culture
III. Cell Culture
IV. Organ Culture
References
3. Construction and Use of Synthetic Media
I. Historical Approach
II. Design of Complete Synthetic Media
III. Synthetic Media in Relation to Cells and their Total Environment
IV. Future Prospects
References
4. Morphological Problems of Cell Type, Shape and Identification
I. The Problem
II. The Importance of Cell Movement
III. The Main Cell Types
IV. The Significance of the Main Cell Types
V. The Possible Evolution of the Cell Types
VI. Some Physico-Chemical Characteristics of the Cell Types
VII. The Problem of Interconversion
VIII. Lessons from the Metaplasia of Naegleria
IX. Cells in Clones and Pure Lines
X. Tissue Culture and the Meaning of Differentiation
References
5. The Locomotory Behaviour of Cells
I. Introduction
II. The Individual Cell
III. Cell Populations
References
6. Cell Division
I. Introduction
II. Mitosis
III. The Cell Cycle
IV. Mitogenesis
V. Synchronism of Divisions in Cultures
VI. Conclusions
References
7. Carbohydrate and Energy Metabolism
I. Introduction
II. Energy Relationships in Growing and Stationary Cells
III. Energy-Yielding Reactions
IV. Relationships Between Energy Metabolism and Other Functions
V. Influence of Environmental Factors
VI. Stable Differences Among Cells
References
8. Amino Acid and Protein Metabolism. I. The Metabolic Characteristics of Serially Propagated Cells
I. Introduction
II. Nutritional Requirements of Serially Propagated Cells
III. Amino Acid and Protein Metabolism in Cell Cultures
IV. General Considerations
References
9. Amino Acid and Protein Metabolism. II. Further Aspects of the Amino Acid and Protein Metabolism of Tissues in Culture
I. Introduction
II. Nutritional Studies
III. Utilization of Amino Acids
IV. Protein Metabolism
V. Conclusion
References
10. Deoxyribonucleic Acid and Ribonucleic Acid Synthesis in Cell Cultures
I. Introduction
II. DNA Synthesis in Cell Cultures
III. RNA Synthesis in Cell Cultures
IV. The Relations Between DNA, RNA and Nuclear Protein During the Interphase of Rapidly Dividing Cells
References
11. Mucopolysaccharide Metabolism in Tissue Culture
I. Introduction
II. Analytical and Technical Methods
III. Tissue Culture Studies
IV. Concluding Remarks
References
12. Genetic Cytology
I. Introduction
II. Cytogenetics of Man
III. DNA Synthesis and the Cell Cycle
IV. Heterochromatin and Heteropyknosis
V. Sex Chromatin, Sex Chromosomes, Sex Determination
References
13. Differentiation: Environmental Factors, Chemical and Cellular
I. Introduction
II. Criteria of Differentiation
III. Current Problems of Differentiation in Vitro
IV. Cultural Factors and Differentiation
V. Homotypic Factors
VI. Heterotypic Factors
VII. Summary and Prospect
References
14. Recombination of Dissociated Cells and the Development of Cell Aggregates
I. Introduction
II. Aggregation of Cells
III. The Development of Aggregates
IV. Remarks on the Internal Patterns of Aggregates
References
15. Embryogenesis in Vitro
I. Culture of Amphibian Embryonic Anlage
II. In Vitro Culture of Embryos of Birds
III. In Vitro Culture of Mammalian Ova
IV. Culture in Vitro of the Embryos of Fish and Reptiles
References
16. The Action of Hormones on Cell and Organ Culture
I. Introduction
II. Effects of Insulin and Growth Hormone
III. Effects of Thyrotrophin (TSH), Adrenocorticotrophin (ACTH) and Gonadotrophins
IV. Action of Corticosteroids and Adrenaline
V. The Effects of Sex Hormones
VI. Influence of Hormones on the Development and Function of the Mouse Mammary Gland
VII. Effects of Thyroxine and Triiodothyronine
VIII. The Effects of Parathyroid Hormone on Bone
IX. Conclusions
References
17. The Effects of Vitamins A and C on Cells and Tissue in Culture
I. Vitamin A
II. Vitamin C
References
Author Index
Subject Index
Product details
- No. of pages: 804
- Language: English
- Copyright: © Academic Press 1965
- Published: January 1, 1965
- Imprint: Academic Press
- eBook ISBN: 9781483223001