Cells and Tissues in Culture

Contributors to Volume 1

Foreword to Volume 1

Contents of Volume 2

Contents of Volume 3


1. Introduction


2. Methods

I. Basic Requirements

II. Tissue Culture

III. Cell Culture

IV. Organ Culture

References


3. Construction and Use of Synthetic Media

I. Historical Approach

II. Design of Complete Synthetic Media

III. Synthetic Media in Relation to Cells and their Total Environment

IV. Future Prospects

References


4. Morphological Problems of Cell Type, Shape and Identification

I. The Problem

II. The Importance of Cell Movement

III. The Main Cell Types

IV. The Significance of the Main Cell Types

V. The Possible Evolution of the Cell Types

VI. Some Physico-Chemical Characteristics of the Cell Types

VII. The Problem of Interconversion

VIII. Lessons from the Metaplasia of Naegleria

IX. Cells in Clones and Pure Lines

X. Tissue Culture and the Meaning of Differentiation

References


5. The Locomotory Behaviour of Cells

I. Introduction

II. The Individual Cell

III. Cell Populations

References


6. Cell Division

I. Introduction

II. Mitosis

III. The Cell Cycle

IV. Mitogenesis

V. Synchronism of Divisions in Cultures

VI. Conclusions

References


7. Carbohydrate and Energy Metabolism

I. Introduction

II. Energy Relationships in Growing and Stationary Cells

III. Energy-Yielding Reactions

IV. Relationships Between Energy Metabolism and Other Functions

V. Influence of Environmental Factors

VI. Stable Differences Among Cells

References


8. Amino Acid and Protein Metabolism. I. The Metabolic Characteristics of Serially Propagated Cells

I. Introduction

II. Nutritional Requirements of Serially Propagated Cells

III. Amino Acid and Protein Metabolism in Cell Cultures

IV. General Considerations

References


9. Amino Acid and Protein Metabolism. II. Further Aspects of the Amino Acid and Protein Metabolism of Tissues in Culture

I. Introduction

II. Nutritional Studies

III. Utilization of Amino Acids

IV. Protein Metabolism

V. Conclusion

References


10. Deoxyribonucleic Acid and Ribonucleic Acid Synthesis in Cell Cultures

I. Introduction

II. DNA Synthesis in Cell Cultures

III. RNA Synthesis in Cell Cultures

IV. The Relations Between DNA, RNA and Nuclear Protein During the Interphase of Rapidly Dividing Cells

References


11. Mucopolysaccharide Metabolism in Tissue Culture

I. Introduction

II. Analytical and Technical Methods

III. Tissue Culture Studies

IV. Concluding Remarks

References


12. Genetic Cytology

I. Introduction

II. Cytogenetics of Man

III. DNA Synthesis and the Cell Cycle

IV. Heterochromatin and Heteropyknosis

V. Sex Chromatin, Sex Chromosomes, Sex Determination

References


13. Differentiation: Environmental Factors, Chemical and Cellular

I. Introduction

II. Criteria of Differentiation

III. Current Problems of Differentiation in Vitro

IV. Cultural Factors and Differentiation

V. Homotypic Factors

VI. Heterotypic Factors

VII. Summary and Prospect

References


14. Recombination of Dissociated Cells and the Development of Cell Aggregates

I. Introduction

II. Aggregation of Cells

III. The Development of Aggregates

IV. Remarks on the Internal Patterns of Aggregates

References


15. Embryogenesis in Vitro

I. Culture of Amphibian Embryonic Anlage

II. In Vitro Culture of Embryos of Birds

III. In Vitro Culture of Mammalian Ova

IV. Culture in Vitro of the Embryos of Fish and Reptiles

References


16. The Action of Hormones on Cell and Organ Culture

I. Introduction

II. Effects of Insulin and Growth Hormone

III. Effects of Thyrotrophin (TSH), Adrenocorticotrophin (ACTH) and Gonadotrophins

IV. Action of Corticosteroids and Adrenaline

V. The Effects of Sex Hormones

VI. Influence of Hormones on the Development and Function of the Mouse Mammary Gland

VII. Effects of Thyroxine and Triiodothyronine

VIII. The Effects of Parathyroid Hormone on Bone

IX. Conclusions

References


17. The Effects of Vitamins A and C on Cells and Tissue in Culture

I. Vitamin A

II. Vitamin C

References

Author Index

Subject Index