Cells and Tissues in Culture

Cells and Tissues in Culture

Methods, Biology and Physiology

1st Edition - January 1, 1965

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  • Editor: E. N. Willmer
  • eBook ISBN: 9781483223001

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Cells and Tissues in Culture: Methods, Biology and Physiology, Volume 1 covers the general fields of tissue culture, including an evaluation of its technique, effects, and contributions to biology. This book focuses on the three methods of culture—tissue culture, cell culture, and organ culture.Other topics include the design of complete synthetic media, possible evolution of the cell types, and energy relationships in growing and stationary cells. The RNA synthesis in cell cultures, culture of amphibian embryonic anlage, action of corticosteroids and adrenaline, and effects of parathyroid hormone on bone are also elaborated. This volume is recommended for biologists and specialists interested in the culture of cells and tissues.

Table of Contents

  • Contributors to Volume 1

    Foreword to Volume 1

    Contents of Volume 2

    Contents of Volume 3

    1. Introduction

    2. Methods

    I. Basic Requirements

    II. Tissue Culture

    III. Cell Culture

    IV. Organ Culture


    3. Construction and Use of Synthetic Media

    I. Historical Approach

    II. Design of Complete Synthetic Media

    III. Synthetic Media in Relation to Cells and their Total Environment

    IV. Future Prospects


    4. Morphological Problems of Cell Type, Shape and Identification

    I. The Problem

    II. The Importance of Cell Movement

    III. The Main Cell Types

    IV. The Significance of the Main Cell Types

    V. The Possible Evolution of the Cell Types

    VI. Some Physico-Chemical Characteristics of the Cell Types

    VII. The Problem of Interconversion

    VIII. Lessons from the Metaplasia of Naegleria

    IX. Cells in Clones and Pure Lines

    X. Tissue Culture and the Meaning of Differentiation


    5. The Locomotory Behaviour of Cells

    I. Introduction

    II. The Individual Cell

    III. Cell Populations


    6. Cell Division

    I. Introduction

    II. Mitosis

    III. The Cell Cycle

    IV. Mitogenesis

    V. Synchronism of Divisions in Cultures

    VI. Conclusions


    7. Carbohydrate and Energy Metabolism

    I. Introduction

    II. Energy Relationships in Growing and Stationary Cells

    III. Energy-Yielding Reactions

    IV. Relationships Between Energy Metabolism and Other Functions

    V. Influence of Environmental Factors

    VI. Stable Differences Among Cells


    8. Amino Acid and Protein Metabolism. I. The Metabolic Characteristics of Serially Propagated Cells

    I. Introduction

    II. Nutritional Requirements of Serially Propagated Cells

    III. Amino Acid and Protein Metabolism in Cell Cultures

    IV. General Considerations


    9. Amino Acid and Protein Metabolism. II. Further Aspects of the Amino Acid and Protein Metabolism of Tissues in Culture

    I. Introduction

    II. Nutritional Studies

    III. Utilization of Amino Acids

    IV. Protein Metabolism

    V. Conclusion


    10. Deoxyribonucleic Acid and Ribonucleic Acid Synthesis in Cell Cultures

    I. Introduction

    II. DNA Synthesis in Cell Cultures

    III. RNA Synthesis in Cell Cultures

    IV. The Relations Between DNA, RNA and Nuclear Protein During the Interphase of Rapidly Dividing Cells


    11. Mucopolysaccharide Metabolism in Tissue Culture

    I. Introduction

    II. Analytical and Technical Methods

    III. Tissue Culture Studies

    IV. Concluding Remarks


    12. Genetic Cytology

    I. Introduction

    II. Cytogenetics of Man

    III. DNA Synthesis and the Cell Cycle

    IV. Heterochromatin and Heteropyknosis

    V. Sex Chromatin, Sex Chromosomes, Sex Determination


    13. Differentiation: Environmental Factors, Chemical and Cellular

    I. Introduction

    II. Criteria of Differentiation

    III. Current Problems of Differentiation in Vitro

    IV. Cultural Factors and Differentiation

    V. Homotypic Factors

    VI. Heterotypic Factors

    VII. Summary and Prospect


    14. Recombination of Dissociated Cells and the Development of Cell Aggregates

    I. Introduction

    II. Aggregation of Cells

    III. The Development of Aggregates

    IV. Remarks on the Internal Patterns of Aggregates


    15. Embryogenesis in Vitro

    I. Culture of Amphibian Embryonic Anlage

    II. In Vitro Culture of Embryos of Birds

    III. In Vitro Culture of Mammalian Ova

    IV. Culture in Vitro of the Embryos of Fish and Reptiles


    16. The Action of Hormones on Cell and Organ Culture

    I. Introduction

    II. Effects of Insulin and Growth Hormone

    III. Effects of Thyrotrophin (TSH), Adrenocorticotrophin (ACTH) and Gonadotrophins

    IV. Action of Corticosteroids and Adrenaline

    V. The Effects of Sex Hormones

    VI. Influence of Hormones on the Development and Function of the Mouse Mammary Gland

    VII. Effects of Thyroxine and Triiodothyronine

    VIII. The Effects of Parathyroid Hormone on Bone

    IX. Conclusions


    17. The Effects of Vitamins A and C on Cells and Tissue in Culture

    I. Vitamin A

    II. Vitamin C


    Author Index

    Subject Index

Product details

  • No. of pages: 804
  • Language: English
  • Copyright: © Academic Press 1965
  • Published: January 1, 1965
  • Imprint: Academic Press
  • eBook ISBN: 9781483223001

About the Editor

E. N. Willmer

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