Cells and Tissues in Culture Methods, Biology and Physiology

Cells and Tissues in Culture Methods, Biology and Physiology

1st Edition - January 1, 1966

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  • Editor: E. N. Willmer
  • eBook ISBN: 9781483277233

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Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures. The selection is a vital source of data for readers interested in the culture of cells and tissues.

Table of Contents

  • Contents

    Contributors to Volume 3

    Foreword to Volume 3

    Foreword to Volume 1

    Foreword to Volume 2

    Contents of Volume 1

    Contents of Volume 2

    1. The Molecular Organization of Cells and Tissues in Culture

    I. Introduction

    II. Methods

    III. Cellular Organization at the Molecular Level

    IV. Fibrogenesis in Tissue Culture


    2. Tissue Culture in Radiobiology

    I. Historical Introduction

    II. The Effects of Radiation on Cell Proliferation

    III. Effects of Radiation on the Growth of Tissue and Cell Cultures

    IV. Effect of Radiation on the Growth of Isolated Cells

    V. Irradiation of Selected Parts of the Cell

    VI. Some Fallacious Methods of Growth Measurement

    VII. Non-mitotic Cell Death

    VIII. Degenerative Changes in the Cytoplasm

    IX. Radiation and Cell Differentiation

    X. The Radiosensitivity of Malignant Cells

    XI. Chemical Factors Influencing Radiosensitivity

    XII. Effects of Radiation on the Culture Medium

    XIII. Comparison of in vitro with in vivo Sensitivity

    XIV. Comparison of Different Sorts of Ionizing Radiation

    XV. Effects of Ultraviolet Radiation

    XVI. Conclusion


    3. Effects of Invading Organisms on Cells and Tissues in Culture

    I. Introduction

    II. Protozoa

    III. Bacteria

    IV. Mycoplasma (PPLO)

    V. Miyagawanella

    VI. Poxviruses

    VII. Herpes Viruses

    VIII. Adenoviruses

    IX. Papova Viruses

    X. Myxoviruses

    XL Avian Sarcomas and Lymphomas

    XII. Picorna Viruses

    XIII. Arboviruses

    XIV. Miscellaneous Viruses

    XV. Apologia and Epilogue


    4. Cell, Tissue and Organ Cultures in Virus Research

    I. Introduction

    II. Isolation of Viruses in Tissue Culture

    III. Identification of Viruses

    IV. Titration of Viruses

    V. Replication of Viruses

    VI. Viral Genetics

    VII. Production of Virus Vaccines

    VIII. Transformation of Cells by Viruses

    IX. Virus Studies in Organ Cultures


    5. Antibody Production in Tissue Culture

    I. Introduction

    II. Early Attempts at in vitro Studies

    III. Recent in vitro Studies

    IV. Combined in vitro-in vivo Studies

    V. Conclusions


    6. Tissue Culture in Pharmacology

    I. Introduction

    II. Methods of Assessment of Drug Activity

    III. Tissue Culture in Screening Systems in Cancer Chemotherapy

    IV. Tissue Culture in the Study of Pharmacologically Active Agents


    7. Invertebrate Tissue and Organ Culture in Cell Research

    I. Introduction

    II. Design of Culture Media

    III. Preparation of Materials and Techniques

    IV. Sources of Cells and Tissues

    V. Growth by Cell Division in Tissue Cultures

    VI. Aggregation of Dissociated Cells

    VII. Culture of Organs and Embryos in Developmental Studies

    VIII. Invertebrate Tissue Culture in Pathology


    8. Introduction and Methods Employed in Plant Tissue Culture

    I. Introduction

    II. Techniques of Organ, Tissue and Free Cell Culture


    9. The Nutrition and Metabolism of Plant Tissue and Organ Cultures

    I. Introduction

    II. Aspects of Inorganic Nutrition

    III. Aspects of Nitrogen Nutrition and Metabolism

    IV. Carbohydrate Requirements and Physiological Effects of Sugars and Sugar Alcohols

    V. Vitamin Nutrition

    VI. Requirements for Growth-regulating Substances

    VII. Pathways of Biosynthesis, "Biochemical Genetics" and Adaptation in Organ and Tissue Cultures

    VIII. The Release of Metabolites into the Culture Medium

    IX. The Resistance to Growth in Culture Encountered with some Organs, Tissues and Cells


    10. Growth, Differentiation and Organogenesis in Plant Tissue and Organ Cultures

    I. Introduction

    II. Growth and Differentiation in Cultured Roots

    III. Histogenesis in Callus Cultures

    IV. Growth and Differentiation in Suspension Cultures

    V. General Conclusions


    11. The Use of Tissue Culture in Phytopathology

    I. Introduction

    II. The Crown-gall Disease

    III. Genetic Tumours

    IV. Virus Tumours

    V. Spruce Tumours

    VI. Fern Tumours

    VII. Insect Galls

    VIII. Nematodes

    IX. Bacterial Diseases

    X. Fungal Diseases

    XI. Virus Diseases


    Author Index

    Taxonomic Index

    Subject Index

Product details

  • No. of pages: 842
  • Language: English
  • Copyright: © Academic Press 1966
  • Published: January 1, 1966
  • Imprint: Academic Press
  • eBook ISBN: 9781483277233

About the Editor

E. N. Willmer

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