Cell Separation

Methods and Selected Applications

1st Edition - January 6, 1987
Editors: Thomas G. Pretlow, Theresa P. Pretlow
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 1 9 4 1 - 7

Cell Separation: Methods and Selected Applications, Volume 4 provides information pertinent to the design and application of methods for the separation of cells. This book covers a… Read more

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Cell Separation: Methods and Selected Applications, Volume 4 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including liver cells, epidermal Langerhans cells, isolation of oval cells, clonal analysis, and the purification of polymorphonuclear leukocytes. Organized into 17 chapters, this volume begins with an overview of the central role of the liver in the metabolism of the body. This text then provides the analysis of Langerhans cells that allow modulation of their function and provide approaches to the treatment of skin disease. Other chapters consider the biological significance of oval cells. This book discusses as well the elucidation of the mechanisms of cellular proliferation, function, and differentiation in living tissues. The final chapter deals with the important applications of cell culture that involve continuous cell lines. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Preface

1. Separation and Characterization of Liver Cells

I. Introduction

II. Characterization of Liver Cells

III. Preparation of Isolated Liver Cells

IV. Conclusions

References

2. Purification and Characterization of Epidermal Langerhans Cells

I. Introduction

II. Cellular Constituents of the Epidermis

III. Characterization of Epidermal Langerhans Cells

IV. Enrichment of Epidermal Langerhans Cells

References

3. Purification and Culture of Oval Cells from Rat Liver

I. Biological Importance of Oval Cells: Does Normal Adult Liver Contain Stem Cells?

II. Isolation and Characterization of Oval Cells and Comparison with Other Liver Cell Types from Normal and Carcinogen-Treated Rats

III. Growth of Liver Epithelial Cells in Vitro

IV. Perspectives

References

4. Isolation and Characterization of Lymphocytes from Mature Mouse Liver

I. Introduction

II. Preparation of Suspensions of Cells from Liver

III. Separation of Lymphocytes from Mouse Liver

IV. Further Characterization of Lymphocytes from Liver

V. Concluding Remarks

References

5. A Comparison of Cell Separations Obtained with Centrifugal Elutriation and Sedimentation at Unit Gravity

I. Introduction

II. A Theoretical Comparison and Factors Influencing Separation

III. Applications

IV. Summary and Conclusions

References

6. Limiting Dilution Assays for the Separation, Characterization, and Quantitation of Biologically Active Particles and Their Clonal Progeny

I. Introduction

II. Sample LDAs: SHPM Validity Tests

III. Sample LDAs: Sample Estimators

IV. Population LDAs

V. Assay Design

VI. Comparative Experiments

VII. Clonal Analysis

VIII. Partition Analysis

IX. Conclusion

References

7. Purification and Functional Evaluation of Human Polymorphonuclear Leukocytes

I. Introduction

II. Background

III. Techniques for the Isolation of Polymorphonuclear Neutrophils

IV. Considerations in Choosing a Technique for the Purification of Polymorphonuclear Neutrophils

V. Functional Analysis of Circulating Human Polymorphonuclear Neutrophils Isolated by Different Methods

VI. Concluding Remarks

References

8. Fabrication of a Manually Operable Countercurrent Distribution Apparatus and Its Application for Separation of Cells in Aqueous Two-Phase Polymer System

I. Introduction

II. Countercurrent Distribution (CCD)

III. Separation of Rat Testicular Cells by Manually Operable CCD Apparatus

IV. Conclusion

References

9. Separation and Characterization of Phagocytes from Human Colon

I. Introduction

II. Dissociation of Mucosal Cells

III. Enrichment of Mucosal Phagocytes

IV. Analysis of Cell Suspensions by Flow Cytometry

V. Functional Analysis

VI. Concluding Remarks

References

10. Exploitation of Surface Molecules for Separation of Cells from Mosaic Livers

I. Introduction

II. Construction of Genotypic Mosaic Livers

III. Immunological Approaches Toward Purifying Donor- and Host-Origin Liver Cells from Genotypic Mosaic Livers

IV. Summary

References

11. Isolation, Characterization, and Possible Functions of Follicular Dendritic Cells from Tonsils and Adenoids

I. Introduction

II. Characteristics of Follicular Dendritic Cells (FDC) in Situ

III. Isolation of FDC

IV. Characterization of Isolated FDC

V. Functional Study of FCD in Vitro

VI. Conclusions

References

12. Magnetite-Protein Conjugates for the Separation of Cells by High Gradient Magnetic Filtration

I. Introduction

II. Magnetic Filtration

III. Particulate Magnetic Labels

IV. Cell Labeling

V. Cell Sorting

VI. Summary

References

13. The Development of Techniques That Permit the Selection and Growth of Malignant Cells from Human Colonic Carcinomas

I. Introduction

II. Tissue Procurement and Initial Tissue Processing

III. Human Colon Cancer Cell Lines

IV. Characterization of Human Colon Cancer Cell Lines

V. Utilization of a Human Cancer Cell Line Bank for Evaluation of New Anticancer Drugs

VI. Concluding Remarks

References

14, Separation of Subpopulations from Heterogeneous Human Monocytes

I. Introduction

II. Separation Procedures Currently Available to Isolate Monocytes

III. Isolation of Monocyte Subsets by Means of a Blood Component Separator (BCS) and Centrifugal Elutriation (CE)

IV. Phenotypic Analysis of the Monocyte Subsets

V. Functional Analysis of the Monocyte Subsets

VI. Conclusions

References

15. Identification and Isolation of Human Splenic Macrophages, Lymphocytes, and Related Cells with in Situ Immunohistochemical Techniques and Countercurrent Centrifugal Elutriation

I. Introduction

II. Identification of Various Types of Spleen Cells in Situ

III. Preparation of Spleen Cell Suspensions

IV. Separation of Spleen Cells by Countercurrent Centrifugal Elutriation

V. Critical Assessment of the Methods Used to Identify Spleen Cell Types and to Separate Them from Splenic Tissue

References

16. Methods for the Purification of Malignant Cells from Blood

I. Introduction

II. General Considerations

III. Separation Procedures

IV. Nonseparative Procedures

V. Conclusions

References

17. Heterogeneity of Proteolytic Enzyme Preparations Commonly Employed for Dispersal of Solid Tissues

I. Introduction

II. Enzymatic Dispersal of Solid Tissues

III. Trypsin

IV. Heterogeneity of Enzyme Preparations Used for Tissue Dispersal

V. Recommendations for Increased Standardization of Tissue Dispersal

References

Index