Cell Separation

Cell Separation

Methods and Selected Applications

1st Edition - January 6, 1987

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  • Editors: Thomas G. Pretlow, Theresa P. Pretlow
  • eBook ISBN: 9781483219417

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Cell Separation: Methods and Selected Applications, Volume 4 provides information pertinent to the design and application of methods for the separation of cells. This book covers a variety of topics, including liver cells, epidermal Langerhans cells, isolation of oval cells, clonal analysis, and the purification of polymorphonuclear leukocytes. Organized into 17 chapters, this volume begins with an overview of the central role of the liver in the metabolism of the body. This text then provides the analysis of Langerhans cells that allow modulation of their function and provide approaches to the treatment of skin disease. Other chapters consider the biological significance of oval cells. This book discusses as well the elucidation of the mechanisms of cellular proliferation, function, and differentiation in living tissues. The final chapter deals with the important applications of cell culture that involve continuous cell lines. This book is a valuable resource for cell biologists, experimental oncologists, hematologists, immunologists, and endocrinologists.

Table of Contents

  • Preface

    1. Separation and Characterization of Liver Cells

    I. Introduction

    II. Characterization of Liver Cells

    III. Preparation of Isolated Liver Cells

    IV. Conclusions


    2. Purification and Characterization of Epidermal Langerhans Cells

    I. Introduction

    II. Cellular Constituents of the Epidermis

    III. Characterization of Epidermal Langerhans Cells

    IV. Enrichment of Epidermal Langerhans Cells


    3. Purification and Culture of Oval Cells from Rat Liver

    I. Biological Importance of Oval Cells: Does Normal Adult Liver Contain Stem Cells?

    II. Isolation and Characterization of Oval Cells and Comparison with Other Liver Cell Types from Normal and Carcinogen-Treated Rats

    III. Growth of Liver Epithelial Cells in Vitro

    IV. Perspectives


    4. Isolation and Characterization of Lymphocytes from Mature Mouse Liver

    I. Introduction

    II. Preparation of Suspensions of Cells from Liver

    III. Separation of Lymphocytes from Mouse Liver

    IV. Further Characterization of Lymphocytes from Liver

    V. Concluding Remarks


    5. A Comparison of Cell Separations Obtained with Centrifugal Elutriation and Sedimentation at Unit Gravity

    I. Introduction

    II. A Theoretical Comparison and Factors Influencing Separation

    III. Applications

    IV. Summary and Conclusions


    6. Limiting Dilution Assays for the Separation, Characterization, and Quantitation of Biologically Active Particles and Their Clonal Progeny

    I. Introduction

    II. Sample LDAs: SHPM Validity Tests

    III. Sample LDAs: Sample Estimators

    IV. Population LDAs

    V. Assay Design

    VI. Comparative Experiments

    VII. Clonal Analysis

    VIII. Partition Analysis

    IX. Conclusion


    7. Purification and Functional Evaluation of Human Polymorphonuclear Leukocytes

    I. Introduction

    II. Background

    III. Techniques for the Isolation of Polymorphonuclear Neutrophils

    IV. Considerations in Choosing a Technique for the Purification of Polymorphonuclear Neutrophils

    V. Functional Analysis of Circulating Human Polymorphonuclear Neutrophils Isolated by Different Methods

    VI. Concluding Remarks


    8. Fabrication of a Manually Operable Countercurrent Distribution Apparatus and Its Application for Separation of Cells in Aqueous Two-Phase Polymer System

    I. Introduction

    II. Countercurrent Distribution (CCD)

    III. Separation of Rat Testicular Cells by Manually Operable CCD Apparatus

    IV. Conclusion


    9. Separation and Characterization of Phagocytes from Human Colon

    I. Introduction

    II. Dissociation of Mucosal Cells

    III. Enrichment of Mucosal Phagocytes

    IV. Analysis of Cell Suspensions by Flow Cytometry

    V. Functional Analysis

    VI. Concluding Remarks


    10. Exploitation of Surface Molecules for Separation of Cells from Mosaic Livers

    I. Introduction

    II. Construction of Genotypic Mosaic Livers

    III. Immunological Approaches Toward Purifying Donor- and Host-Origin Liver Cells from Genotypic Mosaic Livers

    IV. Summary


    11. Isolation, Characterization, and Possible Functions of Follicular Dendritic Cells from Tonsils and Adenoids

    I. Introduction

    II. Characteristics of Follicular Dendritic Cells (FDC) in Situ

    III. Isolation of FDC

    IV. Characterization of Isolated FDC

    V. Functional Study of FCD in Vitro

    VI. Conclusions


    12. Magnetite-Protein Conjugates for the Separation of Cells by High Gradient Magnetic Filtration

    I. Introduction

    II. Magnetic Filtration

    III. Particulate Magnetic Labels

    IV. Cell Labeling

    V. Cell Sorting

    VI. Summary


    13. The Development of Techniques That Permit the Selection and Growth of Malignant Cells from Human Colonic Carcinomas

    I. Introduction

    II. Tissue Procurement and Initial Tissue Processing

    III. Human Colon Cancer Cell Lines

    IV. Characterization of Human Colon Cancer Cell Lines

    V. Utilization of a Human Cancer Cell Line Bank for Evaluation of New Anticancer Drugs

    VI. Concluding Remarks


    14, Separation of Subpopulations from Heterogeneous Human Monocytes

    I. Introduction

    II. Separation Procedures Currently Available to Isolate Monocytes

    III. Isolation of Monocyte Subsets by Means of a Blood Component Separator (BCS) and Centrifugal Elutriation (CE)

    IV. Phenotypic Analysis of the Monocyte Subsets

    V. Functional Analysis of the Monocyte Subsets

    VI. Conclusions


    15. Identification and Isolation of Human Splenic Macrophages, Lymphocytes, and Related Cells with in Situ Immunohistochemical Techniques and Countercurrent Centrifugal Elutriation

    I. Introduction

    II. Identification of Various Types of Spleen Cells in Situ

    III. Preparation of Spleen Cell Suspensions

    IV. Separation of Spleen Cells by Countercurrent Centrifugal Elutriation

    V. Critical Assessment of the Methods Used to Identify Spleen Cell Types and to Separate Them from Splenic Tissue


    16. Methods for the Purification of Malignant Cells from Blood

    I. Introduction

    II. General Considerations

    III. Separation Procedures

    IV. Nonseparative Procedures

    V. Conclusions


    17. Heterogeneity of Proteolytic Enzyme Preparations Commonly Employed for Dispersal of Solid Tissues

    I. Introduction

    II. Enzymatic Dispersal of Solid Tissues

    III. Trypsin

    IV. Heterogeneity of Enzyme Preparations Used for Tissue Dispersal

    V. Recommendations for Increased Standardization of Tissue Dispersal



Product details

  • No. of pages: 398
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: January 6, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9781483219417

About the Editors

Thomas G. Pretlow

Theresa P. Pretlow

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