Capillary Gel Electrophoresis and Related Microseparation Techniques

1st Edition

Author:

  • Andras Guttman
  • Capillary Gel Electrophoresis and Related Microseparation Techniques

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    Description

    Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity based methodologies, micropreparative aspects, as well as related microseparation methods. It gives readers a better understanding of how to utilize this technology, and determine which method will provide the best technical solutions to their particular problems.

    This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology, and biotechnology courses.

    Key Features

    • Covers all theoretical and practical aspects of capillary gel electrophoresis
    • Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity based methodologies, micropreparative aspects, as well as related microseparation methods
    • Teaches readers how to use the technology, and how to select methods ideal for fundamental problems
    • Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology, and biotechnology courses

    Readership

    Academics, industrial analytical chemists, regulatory agencies

    Table of Contents

    Preface
    List of Symbols
    Abbreviations
    1. INTRODUCTION
    1.1. History of gel electrophoresis
    1.2. Modes of capillary gel electrophoresis
    1.3. Basic principles and theory of capillary gel electrophoresis
    1.3.1. Electrophoretic Migration
    1.3.2. Secondary Equilibrium
    1.3.3. Efficiency and Resolution
    1.3.4. Capillary gel electrophoresis of DNA
    1.4. Capillary electrophoresis in anticonvective media
    1.5. Band broadening in capillary gel electrophoresis
    1.6. Power dissipation
    1.7. Comparison with slab gel electrophoresis
    1.8. References
    2. SEPARATION MATRIX AND COLUMN TECHNOLOGY
    2.1. Capillary Coatings
    2.1.1. Covalent coatings
    2.1.2. Physical (non-covalent) surface coverage
    2.2. Separation Matrices
    2.2.1. Cross-linked (chemical) gels, structure and properties
    2.2.2. Non cross-linked linear polymers (physical gels)
    2.2.2.1. Non-cross-linked polyacrylamide and agarose
    2.2.2.2. Derivatized celluloses
    2.2.2.3. Polyethylene oxide and polyvinylpyrrolidone
    2.2.3. Copolymers, composite gels and other alternative matrices
    2.3. Techniques for Preparing Gel Filled Capillaries
    2.3.1. Typical gel formulations
    2.3.2. Choosing the appropriate separation media
    2.4. Alternative Matrices (composite gels, pluronics, sol-gel systems, block polymers)
    2.5. References
    3. INSTRUMENTATION
    3.1. Sample Introduction Methods
    3.1.1. Electrokinetic injection into high viscosity gels
    3.1.2. Pressure and vacuum injection into low viscosity polymer solutions
    3.1.3. Sample stack

    Details

    No. of pages:
    350
    Language:
    English
    Copyright:
    © 2014
    Imprint:
    Elsevier Science
    Print ISBN:
    9780444522344
    Electronic ISBN:
    9780080931357

    About the author

    Andras Guttman

    András Guttman is an adjunct professor at the Barnett Institute in Northeastern University (Boston, MA), also heading the Horváth Laboratory of Bioseparation Sciences (HLBS) in University of Debrecen (Hungary) and recently received the Marie Curie Chair Professorship from the European Commission. As a separation scientist, his fields of research interest is glycomics, biomarker discovery, and microfabricated device technology. Professor Guttman previously held industrial research positions at Novartis (La Jolla, CA), Genetic Biosystems (San Diego, CA), and Beckman Instruments (Fullerton, CA). He has contributed more than 200 scientific publications, 30 book chapters, edited several textbooks, and holds 18 patents. He is the president of the Hungarian Chapter of the American Chemical Society, board member of CASSS, and on the editorial panels of numerous leading international scientific journals such as Analytical Chemistry, Journal of Chromatography, and Electrophoresis. Dr. Guttman graduated from the University of Veszprem (Hungary) in chemical engineering, where he also received his Ph.D. He has received numerous recognitions such as the Analytical Chemistry Award of the Hungarian Chemical Society in 2000, and became a member of the Hungarian Academy of Sciences in 2004.