The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.


Biochemists, biophysicists, cell biologists, and analytical chemists.

Table of Contents

R.S. Givens, J.F.W. Weber, A.H. Jung, and C.-H. Park, New Photoprotecting Groups: Desyl and p-Hydroxyphenacyl Phosphate and Carboxylate Esters. K.R. Gee, B.K. Carpenter, and G.P. Hess, Synthesis, Photochemistry, and Biological Characterization of Photolabile Protecting Groups for Carboxylic Acids and Neurotransmitters. T. Furuta and M. Iwamura, New Caged Groups: 7-Substituted Coumarinylmethyl Phosphate Esters. T.J. Mitchison, K.E. Sawin, J.A. Theriot, K. Gee, and A. Mallavarapu, Caged Fluorescent Probes. R. Sreekumar, M. Ikebe, F.S. Fay, and J.W. Walker, Biologically Active Peptides Caged on Tyrosine. G. Marriott, J. Ottl, M. Heidecker, and D. Gabriel, Light-Directed Activation of Protein Activity from Caged Protein Conjugates. H. Bayley, C.-Y. Chang, W.T. Miller, B. Niblack, and P. Pan, Caged Peptides and Proteins by Targeted Chemical Modification. J. Olejnik, E. Krzymañska-Olejnik, and K.J. Rothschild, Photocleavable Affinity Tags for Isolation and Detection of Biomolecules. G. Marriott and J. Ottl, Synthesis and Applications of Heterobifunctional Photocleavable Cross-Linking Reagents. J.A. McCray, Use of Lasers for One- and Two-Photon Photolysis of Caged Compounds. G. Rapp, Flash Lamp Based Irradiation of Caged Compounds. V. Cepus, C. Ulbrich, C. Allin, A. Troullier, and K. Gerwert, Fourier Transform Infrared Photolysis Studies of Caged Compounds. C.H. Wharton, R.A. Meldrum, and R.S. Chittock, Use of Caged Compounds in Studies of Bioelectronic Imaging and Pattern Recognition. A. Scheidig, C. Burmester, and R.S. Goody, Use of Caged Nucleotides to Characterize Unstable Intermediates by X-Ray Crystallography. L. Peng and M. Goeldner, Photoregulat


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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY