The second part of an updated edition of the classic Methods in Cell Biology, Volume 48, this book emphasizes diverse methods and technologies needed to investigate C. elegans, both as an integrated organism and as a model system for research inquiries in cell, developmental, and molecular biology, as well as in genetics and pharmacology. By directing its audience to tried-and-true and cutting-edge recipes for research, this comprehensive collection is intended to guide investigators of C. elegans for years to come.

Key Features

  • Diverse, up-to-date techniques covered will be useful to the broadening community of C. elegans researchers for years to come
  • Chapters written by leaders in the field
  • Tried and true methods deliver busy researchers a one-stop compendium of essential protocols


Researchers and students in cell, molecular and developmental biology

Table of Contents

High-Resolution Imaging of Cellular Processes in Caenorhabditis elegans

I. Microscope Stand

II. Objective Lenses and Resolution

III. Transmitted Light Imaging

IV. Fluorescence Imaging

V. Detectors

VI. Confocal Imaging

VII. Deconvolution Imaging

VIII. Whole Worm (Adult and Larval Stages)

IX. Embryos

Immunofluorescence Microscopy

I. Introduction

II. Preparing Specimens for Immunolocalization Studies

III. Preparation of Antibodies to Nematode Antigens

IV. Microscopy: Necessary Equipment and Imaging Tips

V. Concluding Remarks

VI. Recipes

Fluorescent Protein Methods: Strategies and Applications

I. Introduction

II. Methods

III. Adopting New Fluorescent Proteins for Use in C. elegans

Modern Electron Microscopy Methods for C. elegans

I. Introduction

II. Modern Electron Microscopes

III. Alternate Immersion Fixation Techniques

IV. Serial Thin Sectioning

V. ImmunoEM Protocols

VI. Alternate Methods to Post Stain Thin Sections

VII. Electron Tomography

VIII. Emerging EM Technologies

IX. Computer-Based Analytical Tools

X. Landmarks for Recognizing Tissues


Culture and Manipulation of Embryonic Cells

I. Introduction: Uses and Limitations of the System

II. Embryo Devitellinization and Blastomere Isolation

III. Culture Methods

IV. Drug Treatments

V. Radioactive Labeling

VI. Fixation, Cytochemistry, and Electron Microscopy

VII. Solutions and Culture Media

Laser Microsurgery in Caenorhabditis elegans

I. Overview

II. Identifying Cells in C. elegans

III. Laser Ablation Theory and Apparatus

IV. Laser Killing of Cells

V. Assessing Damage to the


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Academic Press
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