Biothermodynamics, Part C

Biothermodynamics, Part C

1st Edition - December 28, 2010

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  • Editor: Michael Johnson
  • eBook ISBN: 9780123812698
  • Hardcover ISBN: 9780123812681

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In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. Biothermodynamics, Part C presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an “energy book-keeping system.” While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.

Key Features

  • Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules
  • Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research
  • Offers step-by-step lab instructions, including necessary equipment, from a global research community


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

    1. Measurement and Analysis of Equilibrium Binding Titrations: A beginner’s guide
    2. Dorothy Beckett

    3. Macromolecular Competition Titration Method: Accessing Thermodynamics of the Unmodified Macromolecule – Ligand Interactions Through Spectroscopic Titrations of Fluorescent Analogs
    4. Wlodzimierz Bujalowski and Maria J. Jezewska

    5. Analysis of PKR-RNA interactions by sedimentation velocity
    6. C. Jason Wong, Katherine Launer-Felty and James L. Cole

    7. Structural and Thermodynamic Analysis of PDZ/Ligand Interactions
    8. Tyson R. Shepherd and Ernesto J. Fuentes

    9. Thermodynamic analysis of metal ion-induced protein assembly
    10. Andrew B. Herr and Deborah G. Conrady

    11. Thermodynamic Dissection of Colicin Interactions
    12. Nicholas G Housden and Colin Kleanthous

    13. Energetics of Src homology domain interactions in receptor tyrosine kinase-mediated signaling
    14. John E. Ladbury and Stefan T. Arold

    15. Structural and Functional Energetic Linkages in Allosteric Regulation of Muscle Pyruvate Kinase
    16. J. Ching Lee and Petr Herman

    17. Analysis of Free Energy versus Temperature Curves in Protein Folding and Macromolecular Interactions
    18. Vince J. LiCata and Chin-Chi Liu

    19. Application of the Sequential n-Step Kinetic Mechanism to Polypeptide Translocases
    20. Aaron L. Lucius, Justin M. Miller and Burki Rajendar

    21. A Coupled Equilibrium Approach to Study Nucleosome Thermodynamics
    22. Andrew J. Andrews and Karolin Luger

    23. Quantitative methods for measuring DNA flexibility in vitro and in vivo
    24. Justin P. Peters, Nicole Becker, Emily M. Rueter, Zeljko Bajzer, Jason D. Kahna and L. James Maher

    25. A Thermodynamic Approach for the Targeting of Nucleic Acid Structures Using Their Complementary Single Strands
    26. Hui-Ting Lee, Caroline Carr, Hollie Siebler, Lela Waters, Irine Khutsishvili, Fany Iseka, Brian Domack, Chris M. Olsen, and Luis Marky

    27. Thermodynamics of Biological Processes
    28. Hernan G. Garcia, Jane Kondev, Nigel Orme, Julie Theriot and Rob Phillips

    29. Protein stability in the presence of co-solutes
    30. Luis Marcelo F. Holthauzen, Matthew Auton, Mikhail Sineva and Jorg Rosgen

    31. Small Angle X-ray Scattering Studies of Peptide-Lipid Interactions using the Mouse Paneth Cell α-Defensin Cryptdin-4
    32. Abhijit Mishra, Kenneth P. Tai, Nathan W. Schmidt, André J. Ouellette and Gerard C. L. Wong

    33. Synergy of molecular dynamics and isothermal titration calorimetry in studies of allostery
    34. Rebecca Strawn, Thomas Stockner, Milan Melichercik, Lihua Jin, Wei-Feng Xue, Jannette Carey and Rüdiger Ettrich

    35. Using tryptophan fluorescence to measure the stability of membrane proteins folded in liposomes
    36. C. Preston Moon1 and Karen G. Fleming

    37. Non-B Conformations of CAG Repeats Using 2-aminopurine
    38. Natalya N. Degtyareva and Jeffrey T. Petty

    39. Disulfide bond-mediated passenger domain stalling as a structural probe of autotransporter outer membrane secretion in vivo
    40. Jonathan P. Renn & Patricia L. Clark


    41. Strategies for the Thermodynamic Characterization of Linked Binding/Local Folding Reactions Within the Native State: Application to the LID Domain of Adenylate Kinase from Escherichia coli

    Travis P. Schrank, W. Austin Elam, Jing Li and Vincent J. Hilser

Product details

  • No. of pages: 401
  • Language: English
  • Copyright: © Academic Press 2011
  • Published: December 28, 2010
  • Imprint: Academic Press
  • eBook ISBN: 9780123812698
  • Hardcover ISBN: 9780123812681

About the Serial Volume Editor

Michael Johnson

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

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