Biotechnology and Plant Breeding

Biotechnology and Plant Breeding

Applications and Approaches for Developing Improved Cultivars

1st Edition - January 21, 2014

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  • Editors: Aluízio Borém, Roberto Fritsche-Neto
  • eBook ISBN: 9780124172920
  • Hardcover ISBN: 9780124186729

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Biotechnology and Plant Breeding includes critical discussions of the newest and most important applications of biotechnology in plant breeding, covering key topics such as biometry applied to molecular analysis of genetic diversity, genetically modified plants, and more. This work goes beyond recombinant DNA technology to bring together key information and references on new biotech tools for cultivar development, such as double-haploids, molecular markers, and genome-wide selection, among others. It is increasingly challenging for plant breeders and agricultural systems to supply enough food, feed, fiber and biofuel for the global population. As plant breeding evolves and becomes increasingly sophisticated, a staggering volume of genetic data is now generated. Biotechnology and Plant Breeding helps researchers and students become familiar with how the vast amounts of genetic data are generated, stored, analyzed and applied. This practical resource integrates information about plant breeding into the context of modern science, and assists with training for plant breeders including those scientists who have a good understanding of molecular biology/biotechnology and need to learn the art and practice of plant breeding. Plant biologists, breeding technicians, agronomists, seed technologists, students, and any researcher interested in biotechnologies applied to plant breeding will find this work an essential tool and reference for the field.

Key Features

  • Presents in-depth but easy-to-understand coverage of topics, so plant breeders can readily comprehend them and apply them to their breeding programs
  • Includes chapters that address the already developed and optimized biotechnologies for cultivar development, with real-world application for users
  • Features contributions by authors with several years of experience in their areas of expertise


Scientists, academics and students in plant biology and agronomy, researchers interested in biotechnologies applied to plant breeding, plant breeding technicians and seed technologists

Table of Contents

  • Contributors


    1: Plant Breeding and Biotechnological Advances


    Evolution of Genetics and Plant Breeding

    The Impacts of Advances During the Twentieth Century

    Advances and Expected Benefits of Biotechnology

    Improvement of Tools in the Third Millennium

    Combined Classic Breeding and Biotechnology


    2: Molecular Markers


    PCR-Based Markers

    Hybridization-Based Markers

    Markers Based on Sequencing

    Choice of Molecular Marker

    3: Biometrics Applied to Molecular Analysis in Genetic Diversity


    Genetic Diversity Between Accesses or Within Populations

    Diversity and Population Structure

    Statistic Tests of the Variance Components and Statistics Φ

    4: Genome-Wide Association Studies (GWAS)


    QTL Analysis and Genomic Selection: Concepts

    Genome-Wide Association Studies (GWAS)

    Genome-Wide Mapping via Single Marker Regression

    Statistical Power and Significance of Association for QTL Detection

    Genome-Wide Mapping with Haplotype Mixed Models

    GWAS in Humans

    Capturing h2 in Humans with Imperfect LD Between SNPs and Causal Variants

    5: Genome-Wide Selection (GWS)


    Genome-Wide Selection (GWS)

    Accuracy of GWS

    Estimation, Validation, and Selection Populations

    Relationship Between Genetic Variance and Marker Variance

    Increase in the Efficiency of Selection for Plant Breeding

    6: Genes Prospection


    Principles of Genetic Mapping

    Isolation of Genes by Using Techniques for Structural Genome

    Relationship Between Genetic and Physical Maps

    Physical Mapping and Isolation of Loci of Interest

    The Isolation of Genes From the Functional Genome

    Choosing a Technique for Functional Gene Cloning

    The Cloning of Genes Using Bioinformatics Resources

    Validation of Candidate Genes

    Prospects for the Isolation of Genes of Interest

    7: Tissue Culture Applications for the Genetic Improvement of Plants


    Somaclonal Variation


    Protoplast Fusion

    Embryo Rescue

    Production of Double Haploid Lines

    Synthetic Seed Production

    In vitro Selection

    Germplasm Conservation And Exchange

    8: Transgenic Plants


    Organization and Gene Expression in Eukaryotes

    Manipulation of Nucleic Acids

    Methodologies for the Development of Transgenic Plants

    Laboratory Steps for the Development of Transgenic Plants

    Identification of Transgenic Plants

    Use, Effects, and Management of Transgenic Cultivars

    9: Double Haploids

    Haploid Production

    Ploid Identification and Chromosome Counting

    Double-Haploid Production

    Double Haploids in Breeding

    Advantages and Disadvantages in the Use of Double Haploids

    Application on the Genomic Statistics

    Economical Aspects

    10: Tools for the Future Breeder


    Genomic Tools

    Techniques Applied to Structural Genome

    Techniques Applied to Functional Genome

    Average- And Large-Scale Attainment of Markers

    Bioinformatics Tools

    Biotechnology in Plant Breeding

    Perspectives of Genome-Assisted Plant Breeding


Product details

  • No. of pages: 270
  • Language: English
  • Copyright: © Academic Press 2014
  • Published: January 21, 2014
  • Imprint: Academic Press
  • eBook ISBN: 9780124172920
  • Hardcover ISBN: 9780124186729

About the Editors

Aluízio Borém

Affiliations and Expertise

Departamento de Fitotecnia, Universidade Federal de Vicosa, Brazil

Roberto Fritsche-Neto

Affiliations and Expertise

Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de Sao Paulo

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