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Biotechnology and Plant Breeding - 1st Edition - ISBN: 9780124186729, 9780124172920

Biotechnology and Plant Breeding

1st Edition

Applications and Approaches for Developing Improved Cultivars

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Editors: Aluízio Borém Roberto Fritsche-Neto
Hardcover ISBN: 9780124186729
eBook ISBN: 9780124172920
Imprint: Academic Press
Published Date: 18th February 2014
Page Count: 270
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Biotechnology and Plant Breeding includes critical discussions of the newest and most important applications of biotechnology in plant breeding, covering key topics such as biometry applied to molecular analysis of genetic diversity, genetically modified plants, and more. This work goes beyond recombinant DNA technology to bring together key information and references on new biotech tools for cultivar development, such as double-haploids, molecular markers, and genome-wide selection, among others.

It is increasingly challenging for plant breeders and agricultural systems to supply enough food, feed, fiber and biofuel for the global population. As plant breeding evolves and becomes increasingly sophisticated, a staggering volume of genetic data is now generated. Biotechnology and Plant Breeding helps researchers and students become familiar with how the vast amounts of genetic data are generated, stored, analyzed and applied.

This practical resource integrates information about plant breeding into the context of modern science, and assists with training for plant breeders including those scientists who have a good understanding of molecular biology/biotechnology and need to learn the art and practice of plant breeding. Plant biologists, breeding technicians, agronomists, seed technologists, students, and any researcher interested in biotechnologies applied to plant breeding will find this work an essential tool and reference for the field.

Key Features

  • Presents in-depth but easy-to-understand coverage of topics, so plant breeders can readily comprehend them and apply them to their breeding programs
  • Includes chapters that address the already developed and optimized biotechnologies for cultivar development, with real-world application for users
  • Features contributions by authors with several years of experience in their areas of expertise


Scientists, academics and students in plant biology and agronomy, researchers interested in biotechnologies applied to plant breeding, plant breeding technicians and seed technologists

Table of Contents



1: Plant Breeding and Biotechnological Advances


Evolution of Genetics and Plant Breeding

The Impacts of Advances During the Twentieth Century

Advances and Expected Benefits of Biotechnology

Improvement of Tools in the Third Millennium

Combined Classic Breeding and Biotechnology


2: Molecular Markers


PCR-Based Markers

Hybridization-Based Markers

Markers Based on Sequencing

Choice of Molecular Marker

3: Biometrics Applied to Molecular Analysis in Genetic Diversity


Genetic Diversity Between Accesses or Within Populations

Diversity and Population Structure

Statistic Tests of the Variance Components and Statistics Φ

4: Genome-Wide Association Studies (GWAS)


QTL Analysis and Genomic Selection: Concepts

Genome-Wide Association Studies (GWAS)

Genome-Wide Mapping via Single Marker Regression

Statistical Power and Significance of Association for QTL Detection

Genome-Wide Mapping with Haplotype Mixed Models

GWAS in Humans

Capturing h2 in Humans with Imperfect LD Between SNPs and Causal Variants

5: Genome-Wide Selection (GWS)


Genome-Wide Selection (GWS)

Accuracy of GWS

Estimation, Validation, and Selection Populations

Relationship Between Genetic Variance and Marker Variance

Increase in the Efficiency of Selection for Plant Breeding

6: Genes Prospection


Principles of Genetic Mapping

Isolation of Genes by Using Techniques for Structural Genome

Relationship Between Genetic and Physical Maps

Physical Mapping and Isolation of Loci of Interest

The Isolation of Genes From the Functional Genome

Choosing a Technique for Functional Gene Cloning

The Cloning of Genes Using Bioinformatics Resources

Validation of Candidate Genes

Prospects for the Isolation of Genes of Interest

7: Tissue Culture Applications for the Genetic Improvement of Plants


Somaclonal Variation


Protoplast Fusion

Embryo Rescue

Production of Double Haploid Lines

Synthetic Seed Production

In vitro Selection

Germplasm Conservation And Exchange

8: Transgenic Plants


Organization and Gene Expression in Eukaryotes

Manipulation of Nucleic Acids

Methodologies for the Development of Transgenic Plants

Laboratory Steps for the Development of Transgenic Plants

Identification of Transgenic Plants

Use, Effects, and Management of Transgenic Cultivars

9: Double Haploids

Haploid Production

Ploid Identification and Chromosome Counting

Double-Haploid Production

Double Haploids in Breeding

Advantages and Disadvantages in the Use of Double Haploids

Application on the Genomic Statistics

Economical Aspects

10: Tools for the Future Breeder


Genomic Tools

Techniques Applied to Structural Genome

Techniques Applied to Functional Genome

Average- And Large-Scale Attainment of Markers

Bioinformatics Tools

Biotechnology in Plant Breeding

Perspectives of Genome-Assisted Plant Breeding



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© Academic Press 2014
18th February 2014
Academic Press
Hardcover ISBN:
eBook ISBN:

About the Editors

Aluízio Borém

Affiliations and Expertise

Departamento de Fitotecnia, Universidade Federal de Vicosa, Brazil

Roberto Fritsche-Neto

Affiliations and Expertise

Departamento de Genética, Escola Superior de Agricultura "Luiz de Queiroz", Universidade de Sao Paulo

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