Biosensors for Single-Cell Analysis

Biosensors for Single-Cell Analysis

1st Edition - October 26, 2021

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  • Editors: Jian Chen, Yao Lu
  • eBook ISBN: 9780323885591
  • Paperback ISBN: 9780323898416

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Description

Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers.

Key Features

  • Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization
  • Features case studies that illustrate the application of biosensors for single-cell analysis
  • Considers microfluidic approaches for each single-cell property discussed
  • Explores future directions for single-cell analysis and biosensor technology

Readership

Molecular biologists, biochemists, bioengineers and related professionals working with biosensors, single-cell analysis and microfluidics. Researchers and postgraduate/PhD students in biophysics, biochemistry, cell biology, molecular biology and physiology; biomedical engineers

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • Contributors
  • Foreword
  • Acknowledgment
  • Chapter 1: Introduction to biosensors for single-cell analysis with historical developments highlighted
  • Abstract
  • Acknowledgment
  • 1: Introduction to biosensors for single-cell analysis
  • 2: Historical developments of biosensors for single-cell analysis
  • 3: Book structure
  • References
  • Chapter 2: Biosensors for single-cell proteomic characterization
  • Abstract
  • Acknowledgment
  • 1: Introduction
  • 2: Antibody-based SCP characterization by microfluidic biosensor
  • 3: Substrate-based characterization of SCP by microfluidic biosensor
  • 4: Chip-MS for SCP characterization
  • 5: Commercial microfluidic-based biosensing platform for SCP characterization
  • 6: Conclusion and perspectives
  • References
  • Chapter 3: Biosensors for single-cell metabolomic characterization
  • Abstract
  • Acknowledgment
  • 1: Introduction
  • 2: Fluorescence
  • 3: Electrochemical detection
  • 4: Mass spectrometry
  • 5: Conclusion
  • References
  • Chapter 4: Single-cell electrical characterization
  • Abstract
  • Acknowledgment
  • 1: Introduction
  • 2: Basic theory
  • 3: Dielectrophoresis (DEP) and electrorotation (ROT)
  • 4: Microfluidic impedance cytometry
  • 5: Recent trends and future developments
  • References
  • Chapter 5: Biosensors for single-cell mechanical characterization
  • Abstract
  • 1: Introduction
  • 2: Biology background
  • 3: Importance of sing-cell mechanical phenotyping
  • 4: Conventional methods
  • 5: Microfluidics-based methods
  • 6: Summary
  • References
  • Chapter 6: Single-cell analysis by evanescent wave sensing and hyperspectral microscopy
  • Abstract
  • Acknowledgment
  • 1: Introduction
  • 2: Label-free optical refractometric biosensors for SCA
  • 3: Hyperspectral microscopic imaging methods for SCA
  • 4: Conclusion
  • References
  • Chapter 7: Future directions of biosensors for single-cell analysis from the perspective of instrument developments
  • Abstract
  • Acknowledgment
  • 1: Introduction
  • 2: Instrumentation of hematology analyzer (Table 1)
  • 3: Instrumentation of flow cytometer (Table 2)
  • 4: Future directions
  • Index

Product details

  • No. of pages: 182
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: October 26, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780323885591
  • Paperback ISBN: 9780323898416

About the Editors

Jian Chen

Professor Jian Chen received his PhD from the Department of Biomedical Engineering, University of Toronto. Following this he joined the State Key Laboratory of Transducer Technology, Chinese Academy of Sciences where he has been focusing on exploring biosensors for single-cell analysis, enabling high-throughput characterizations of single-cell electrical, mechanical and proteomic properties. He contributed to the publication of approximately 90 journal papers and filed the applications of 40 patents. Among other honours, Professor Chen was the recipient of National Natural Science Foundation of China for Excellent Young Scholars, Excellent Member of Youth Innovation Promotion Association of Chinese Academy of Sciences, and Beijing NOVA Star. Among other services, Professor Chen has served as committee members of micro/nano device and system division of MEMS & NEMS society of China and life science instrument division of the Chinese society of biotechnology.

Affiliations and Expertise

Associate Professor, State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, China

Yao Lu

Dr. Yao Lu is the group leader of single cell analysis lab at Dalian Institute of Chemical Physics, Chinese Academy of Sciences (DICP, CAS). He received his PhD in analytical chemistry from the same institute in 2010. After conducting postdoctoral training at Yale University from 2010 to 2015, he joined the faculty of DICP in September of 2015. His current research is focused on developing microfluidic based single cell analysis technologies for personalized diagnostics and medicine.

Affiliations and Expertise

Associate Professor, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Beijing, China

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