Biophysical Tools for Biologists - 1st Edition - ISBN: 9780123725202, 9780080554945

Biophysical Tools for Biologists, Volume 84

1st Edition

In Vitro Techniques

Editors: John Correia H. William Detrich, III
eBook ISBN: 9780080554945
Hardcover ISBN: 9780123725202
Imprint: Academic Press
Published Date: 5th November 2007
Page Count: 1014
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Table of Contents

Part I: History and Reflections

Section 1. Solution Methods

  1. Binding: A Polemic and Rough Guide Nichola C. Garbett, Jonathan B. Chaires

  2. Linked Equilibria in Regulation of Transcription Initiation Dorothy Beckett

  3. Biosensor-Surface Plasmon Resonance Methods for Quantitative Analysis of Biomolecular Interactions Farial A. Tanious, Binh Nguyen, and W. David Wilson

  4. Isothermal Titration Calorimetry: Experimental Design, Data Analysis, and Probing Macromolecule/Ligand Binding and Kinetic Interactions Matthew W. Freyer and Edwin A. Lewis

  5. Differential Scanning Calorimetry Charles H. Spink

  6. Analytical Ultracentrifugation: A) Sedimentation Velocity, B) Sedimentation Equilibrium Tom Laue, Tom Moody, James Cole, and Jeff Lary

  7. Determination of membrane protein molecular weights and association equilibrium constants using sedimentation equilibrium and sedimentation velocity Nancy K. Burgess, Ann Marie Stanley, and Karen G. Fleming

  8. Basic Aspects of Absorption and Fluorescence Spectroscopy and Resonance Energy Transfer Methods Susan Bane and Natasha Shanker

  9. Applications of Fluorescence Anisotropy to the Study of Protein-DNA Interactions Vince J. LiCata and Andy J. Wowor

  10. Circular Dichroism and Its Application to the Study of Biomolecules Stephen R. Martin and Maria J. Schilstra

  11. Folding and Stability Timothy O. Street, Naomi Courtemanche and Doug Barrick

  12. Hydrodynamic shape modelling of analytical ultracentrifugation data Olwyn Byron

  13. X-ray and neutron scattering data and their constrained molecular modelling Stephen J Perkins, Azubuike I. Okemefuna, Anira N. Fernando, Alexandra Bonner, Hannah E. Gilbert and Patricia B. Furtado

  14. Structural Investigations into Microtubule-MAP Complexes Andreas Hoenger and Heinz Gross

  15. Rapid Kinetic Techniques John F. Eccleston, Stephen Martin, and Maria J. Schilstra

  16. Mutagenic Analysis of the Membrane Protein Functional Mechanisms: Bacteriorhodopsin as a Model Example George J. Turner

  17. Quantifying DNA-protein interactions by single molecule stretching Mark C. Williams, Ioulia Rouzina, and Richard L. Karpel

  18. Isotopomer-based metabolomic analysis by NMR and mass spectrometry Andrew N Lane, Teresa W-M. Fan, and Richard M. Higashi

  19. Following molecular transitions with single residue spatial and millisecond time resolution Inna Shcherbakova, Somdeb Mitra, Robert Beer and Michael Brenowitz

  20. Methods and applications of site-directed spin labeling EPR spectroscopy Candice S. Klug and Jimmy B. Feix

  21. Fluorescence Correlation Spectroscopy and its application to the characterization of molecular properties and interactions Hacène Boukari and Dan L. Sackett

  22. Practical Guide to Osmolytes Jorg Rosgen and Daniel Harries

  23. Stupid Statistics Joel Tellinghuisen

  24. Nonlinear Least Squares Fitting Methods Michael L. Johnson

  25. Methods for Simulating the Dynamics of Complex Biological Processes Maria J Schilstra, Stephen R. Martin, and Sarah M. Keating

  26. Computational Methods for Biomolecular Electrostatics Feng Dong, Brett Olsen and Nathan A. Baker

  27. Ligand effects on the protein ensemble: Unifying the descriptions of ligand binding, local conformational fluctuations, and protein stability Steven T Whitten, Bertrand García-Moreno E., and Vincent J. Hilser

  28. Molecular Modeling of Cytoskeletal Proteins Xiange Zheng and David Sept

  29. Mathematical Modeling of Cell Motility Anders E. Carlsson and David Sept


Description

Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.

Key Features

  • Describes techniques in the context of important biological problems
  • Delineates critical steps and potential pitfalls for each method
  • Includes full-color plates to illustrate techniques

Readership

Cell biologists, biophysicists, pharmacologists, and molecular biologists.


Details

No. of pages:
1014
Language:
English
Copyright:
© Academic Press 2007
Published:
Imprint:
Academic Press
eBook ISBN:
9780080554945
Hardcover ISBN:
9780123725202

Ratings and Reviews


About the Editors

John Correia Editor

Affiliations and Expertise

University of Mississippi Medical Center, Jackson, USA

H. William Detrich, III Editor

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Northeastern University, Boston, MA, USA