Biological NMR Part A

Biological NMR Part A

1st Edition - January 3, 2019
This is the Latest Edition
  • Editor: A.Joshua Wand
  • eBook ISBN: 9780128138618
  • Hardcover ISBN: 9780128138601

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Biological NMR, Part A, the latest release in the Methods of Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Protein methyl labeling, Membrane protein expression – yeast, Protein aromatic labeling, His-tag/Metal contamination, Bicelles, nanodiscs and micelles MP host, PTM – phosphorylation, PTM – lipidation, Screening platform for receptor-ligand discovery Solution Spectroscopy, Large protein strategies, NUS data collection/analysis, F19 incl. hydration, ODNP - hydration, Reverse micelle - Hydration Solid State Spectroscopy, SS NMR membrane proteins, SS NMR soluble/aggregate proteins, SS DNP - general, SS NMR nucleic acids, Structure determination and computer analysis, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods of Enzymology series
  • Updated release includes the latest information on the Biological NMR


Addressed to all specialists in the field

Table of Contents

  • 1. Selective Methyl Labeling of Proteins: Enabling Structural and Mechanistic Studies As Well As Drug Discovery Applications by Solution-State NMR
    Andrew Proudfoot, Andreas O. Frank, Alexandra Frommlet and Andreas Lingel
    2. Isotopic Labeling of Eukaryotic Membrane Proteins for NMR Studies of Interactions and Dynamics
    Igor Dikiy, Lindsay Clark, Kevin H. Gardner and Daniel M. Rosenbaum
    3. Optimal Isotope Labeling of Aromatic Amino Acid Side Chains for NMR Studies of Protein Dynamics
    Ulrich Weininger
    4. A Quick and Colorful Method to Measure Low-Level Contaminations of Paramagnetic Ni2+ in Protein
    Samples Purified by Immobilized Metal Ion Affinity Chromatography
    Starla D. Glover and Cecilia Tommos
    5. Toolkit for NMR Studies of Methyl-Labeled Proteins
    Paolo Rossi, Yoan R. Monneau, Youlin Xia, Yojiro Ishida and Charalampos G. Kalodimos
    6. Synthetic Biology-Based Solution NMR Studies on Membrane Proteins in Lipid Environments
    Erik Henrich, Frank Löhr, Julija Mezhyrova, Aisha Laguerre, Frank Bernhard and Volker Dötsch
    7. Preparation of Phosphorylated Proteins for NMR Spectroscopy
    Ganesan Senthil Kumar, Rebecca Page and Wolfgang Peti
    8. Synthesis and NMR Characterization of the Prenylated Peptide, a-Factor
    Taysir K. Bader, Todd M. Rappe, Gianlugi Veglia and Mark D. Distefano
    9. The Preparation and Solution NMR Spectroscopy of Human Glycoproteins Is Accessible and Rewarding
    Adam W. Barb, Daniel J. Falconer and Ganesh P. Subedi
    10. Nonuniform Sampling for NMR Spectroscopy
    Scott Robson, Haribabu Arthanari, Sven G. Hyberts and Gerhard Wagner
    11. Exploring Protein Conformational Landscapes Using High-Pressure NMR
    Julien Roche, Catherine A. Royer and Christian Roumestand
    12. CS-ROSETTA
    Santrupti Nerli and Nikolaos G. Sgourakis
    13. Combining Evolutionary Covariance and NMR Data for Protein Structure Determination
    Yuanpeng Janet Huang, Kelly P. Brock, Yojiro Ishida, Gurla V.T. Swapna, Masayori Inouye, Debora S. Marks, Chris Sander and Gaetano T. Montelione
    14. Combining NMR Spectroscopy and Molecular Dynamic Simulations to Solve and Analyse the Structure of Protein–RNA Complexes
    Sebastien Campagne, Miroslav Krepl, Jiri Sponer and Frederic H-T. Allain

Product details

  • No. of pages: 440
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: January 3, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128138618
  • Hardcover ISBN: 9780128138601

About the Editor

A.Joshua Wand

Dr. A. Joshua Wand teaches at the Department of Biochemistry & Biophysics, Perelman School of Medicine, University of Pennsylvania, USA

Affiliations and Expertise

University of Pennsylvania, USA