Bioinformatics for Everyone

Bioinformatics for Everyone

1st Edition - September 14, 2021

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  • Authors: Mohammad Sofi, Afshana Shafi, Khalid Masoodi
  • Paperback ISBN: 9780323911283
  • eBook ISBN: 9780323911290

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Bioinformatics for Everyone provides a brief overview on currently used technologies in the field of bioinformatics—interpreted as the application of information science to biology— including various online and offline bioinformatics tools and softwares. The book presents valuable knowledge in a simplified way to help students and researchers easily apply bioinformatics tools and approaches to their research and lab routines. Several protocols and case studies that can be reproduced by readers to suit their needs are also included.

Key Features

  • Explains the most relevant bioinformatics tools available in a didactic manner so that readers can easily apply them to their research
  • Includes several protocols that can be used in different types of research work or in lab routines
  • Discusses upcoming technologies and their impact on biological/biomedical sciences


Graduate students using bioinformatics to deal with research data

Table of Contents

  • 1. Prologue to bioinformatics
    2. Advances in DNA sequencing
    3. Bioinformatics databases and tools
    4. Nucleic acid sequence databases
    5. Pairwise sequence alignment
    6. Multiple sequence alignment
    7. Multiple sequence alignment tools - software and resources
    8. CLUSTALW software
    9. Plant genomic data and resources at NCBI
    10. NCBI BLAST
    11. BLAST: protocols
    12. ExPASy portal
    13. Primer designing tools
    14. Primer designing for cloning
    15. Restriction analysis tools
    16. Restriction analysis using NEBcutter
    17. KEGG database
    18. Database for annotation, visualisation and integrated discovery
    19. Genome analysis browsers
    20. Next-generation alignment tools
    21. Molecular marker storage databases and data visualisation
    22. Introduction to computer-aided drug design
    23. BioEdit in bioinformatics

Product details

  • No. of pages: 266
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: September 14, 2021
  • Imprint: Academic Press
  • Paperback ISBN: 9780323911283
  • eBook ISBN: 9780323911290

About the Authors

Mohammad Sofi

Mohammad Yaseen Sofi is a PhD scholar at Division of Plant Biotechnology, SKUAST- K. He completed his Bachelor's degree from SKUAST-K in 2018. He is currently working under the guidance Dr. Khalid Z. Masoodi on bioprospecting and DNA barcoding of Kale (Brassica oleracea var Acephala). In the course of his MSc programme, he made outstanding contributions in rice research in the field of Plant Biotechnology. He has filed one US patent application in 2021. Additionally, he has extensive knowledge of bioinformatics analysis tools, which he acquired while attending various national/international seminars and workshops.

Affiliations and Expertise

PhD Student, Transcriptomics Lab (K-Lab), Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India

Afshana Shafi

Afshana Shafi did her Bachelors from SKUAST-K, followed by Masters in Plant Biotechnology from SKUAST-Kashmir, Shalimar, J&K, India. Currently, she is pursuing Ph.D from Division of Plant Biotechnology, SKUAST- K, on "Mining and Expression Analysis of Differentially Expressing Genes between selected scab resistant and susceptible apple (Malus domestica Borkh) cultivars” under the guidance of Dr Khalid Masoodi. She conducted an extensive study on Expression Profiling of Near-Isogenic Lines (NILs) Carrying Blast Resistance Genes Pi9 and Pi54 in the Background of Rice Cultivar Mushk Budji as part of her MSc research programme. From achieving the twentieth position in the state of J&K during her tenth board examination to achieving the first rank during her bachelor's programme, she has always been a meritorious student with an outstanding academic and extracurricular record. In 2017-18, she was awarded with BSc. merit scholarship by SKUAST-K. Additionally during the MSc programme Afshana Shafi has obtained second position. Moreover, she has a strong hold on a variety of bioinformatic analytic softwares, which she acquired throughout her studies in the field of Biotechnology and bioinformatics.

Affiliations and Expertise

PhD Student, Transcriptomics Lab (K-Lab), Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, J&K, India

Khalid Masoodi

Dr. Khalid Zaffar Masoodi is a young scientist who works as an Assistant Professor in the Division of Plant Biotechnology at the Sher-e Kashmir University of Agricultural Sciences and Technology in Kashmir (India). He holds a Master's degree in Biotechnology and a doctorate in Plant Biotechnology. Dr. Masoodi is the recipient of numerous awards, including the SERB Early Career Research Award 2016, the SBUR Travel Award in the United States of America in 2012, and the Society of Endocrinology Journal Award in 2018 at Glasgow United Kingdoms. Dr. Masoodi has a distinguished academic record, having qualified for the CSIR-UGC net in Life Sciences, the DBT-BET JRF, and the Graduate Aptitude Test in Engineering and Sciences (GATE) while doing his Masters in Biotechnology. Dr. Masoodi has filed Six patent applications in last 2 years, has over 12 years of teaching and research experience, and has published numerous scientific articles in the fields of cancer research, human disease research, and biotechnology research. His laboratory (Transcriptomics Laboratory, often known as K-Lab, after the Klenow fragment) at SKUAST-K is fully equipped with fluorescence imaging, real-time PCR, cell culture, and Western blotting including other state-of-the-art equipment. His research interests span multiple dimensions, including transcriptomics of plant stress biology (biotic/abiotic) and cancer biology. Apart from that, he is extensively involved in the research of new drugs from medicinal plants. Additionally, he is a co-author of Advanced Molecular Biology and Biotechnology Methods (Academic Press, 2020).

Affiliations and Expertise

Assistant Professor/Junior Scientist at the Division of Plant Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India

Ratings and Reviews

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  • Harris A. Thu Sep 02 2021

    A good book to read

    Seeing the content of this books makes me feel that this type of simple bioinformatics book should have come out earlier. Authors have done true justice with the book. I feel elated that in the time when books are releasing at a rate not seen before this books finds its place in hall of fame for being out basic stuff that everyone can do. And every beginner scientist needs this type of bioinformatics. I congratulate the authors.

  • James M. Thu Jul 01 2021

    A great contribution

    I had a chance to look at the contents of this book and has been written in a lucid and user friendly way. The hands on experiment are easily doable and of great help to students and researchers