Biofabrication

Preface

List of Contributors

Chapter 1. In Vitro Biofabrication of Tissues and Organs

Introduction

1.1 Problems with scaffold-based tissue engineering

1.2 “Scaffold-free” tissue engineering

1.3 Aggregation/spheroid-based approaches

Conclusion

References

Chapter 2. Biomaterials for Biofabrication of 3D Tissue Scaffolds

Introduction

2.1 Materials for 3D tissue scaffolds

2.2 Fabrication methods for 3D tissue scaffolds

2.3 Application of 3D tissue scaffolds

2.4 Future perspectives of biomaterials for 3D scaffolds

Conclusion

Acknowledgment

References

Chapter 3. Projection Printing of Three-Dimensional Tissue Scaffolds with Tunable Poisson’s Ratio

Introduction

3.1 Digital micromirror-assisted projection printing stereolithography

3.2 Negative Poisson’s ratio biomaterials

3.3 Hybrid Poisson’s ratio biomaterial

3.4 Zero Poisson’s ratio biomaterials

Conclusion

References

Chapter 4. Fabrication of Microscale Hydrogels for Tissue Engineering Applications

Introduction

4.1 Technologies for fabrication of microengineered hydrogels

4.2 Tissue engineering applications

Conclusion

Acknowledgments

References

Chapter 5. Polymeric Membranes for the Biofabrication of Tissues and Organs

Introduction

5.1 Properties of polymeric membranes

5.2 Membranes for tissue engineering

5.3 Hollow fiber polymeric membranes for cell and tissue perfusion

5.4 Membrane bioreactors for tissue constructs

5.5 Future perspectives

References

Chapter 6. Laser-Assisted Bioprinting for Tissue Engineering

Introduction

6.1 LAB’s terms of reference

6.2 LAB parameters for cell printing

6.3 High-resolution/high-throughput trade-offs

6.4 Three-dimensional printing: the layer-by-layer approach

6.5 Applications

Conclusion

Acknowledgments

References

Further Reading

Chapter 7. The Modular Approach

Introduction

7.1 Materials used to fabricate the modular building blocks

7.2 Intrinsically vascularized tissue constructs

7.3 Building tissue constructs by assembling building blocks

7.4 Modular tissue engineering combined with microfluidics

Conclusion

References

Chapter 8. Formation of Multicellular Microtissues and Applications in Biofabrication

Introduction

8.1 Materials and methods

8.2 Results

8.3 Discussion

8.4 Future considerations

References

Chapter 9. A Digital Microfabrication-Based System for the Fabrication of Cancerous Tissue Models

Introduction

9.1 Digital micro-mirroring microfabrication system

9.2 Multinozzle biologics deposition system

9.3 Methods and materials

9.4 Results

Conclusion

References

Chapter 10. Breast Reconstruction Using Biofabrication-Based Tissue Engineering Strategies

Introduction

10.1 Anatomy of the breast

10.2 Current methods of breast reconstruction

10.3 Biofabrication-based tissue engineering strategy

Conclusion

References

Chapter 11. Fabrication of Artificial Bacteria for Targeted Drug Delivery

Introduction

11.1 Drug delivery

11.2 Bioinspired locomotion and microswimming

11.3 Materials and methods

Conclusion

Acknowledgment

References

Chapter 12. Biofabricating the Bio-Device Interface Using Biological Materials and Mechanisms

Introduction

12.1 Complementarity of biofabrication and microfabrication

12.2 Biofabrication methods

12.3 Biofabrication of a multifunctional matrix

12.4 Postfabrication biofunctionalization of devices

12.5 Test device

12.6 Electroaddressing a biosensing enzyme

12.7 Creation of a spatially segregated bacterial biofilm

Conclusion

Acknowledgment

References

Index

Biofabrication is a practical guide to the novel, inherently cross-disciplinary scientific field that focuses on biomanufacturing processes and a related range of emerging technologies. These processes and technologies ultimately further the development of products that may involve living (cells and/or tissues) and nonliving (bio-supportive proteins, scaffolds) components. The book introduces readers to cell printing, patterning, assembling, 3D scaffold fabrication, cell/tissue-on-chips as a coherent micro-/nano-fabrication toolkit. Real-world examples illustrate how to apply biofabrication techniques in areas such as regenerative medicine, pharmaceuticals and tissue engineering.

In addition to being a vital reference for scientists, engineers and technicians seeking to apply biofabrication techniques, this book also provides an insight into future developments in the field, and potential new applications.

• Discover the multi-disciplinary toolkit provided by biofabrication and apply it to develop new products, techniques and therapies
• Covers a range of important emerging technologies in a coherent manner: cell printing, patterning, assembling, 3D scaffold fabrication, cell/tissue-on-chips...
• Readers develop the ability to apply biofabrication technologies through practical examples

R&D teams, researchers and

scientists in the fields of

Bioengineering / Biomaterials /

Biofabrication / Biomedical

Engineering / Tissue Engineering;

Regenerative Medicine,

Biotechnology, Pharmaceuticals,

Mechanical Engineering.

Engineers and Industrial scientists at

pharmaceutical companies, biotech

companies, medical device

companies.

Biotechnologists and Process

E