Bioconjugate Techniques, 3rd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions, with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.

Key Features

  • Offers a one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab
  • Provides step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates
  • Features full color illustrations
  • Includes a more extensive introduction into the vast field of bioconjugation and one of the most thorough overviews of immobilization chemistry ever presented


Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology

Table of Contents


Preface to the Third Edition


Important Information

Health and Safety

Intellectual Property

Chapter 1. Introduction to Bioconjugation

1 What is Bioconjugation?

2 Bioconjugation Strategy and Design

3 The Applications of Bioconjugates

4 Summary

Chapter 2. Functional Targets for Bioconjugation

1 Modification of Amino Acids, Peptides, and Proteins

2 Modification of Sugars, Polysaccharides, and Glycoconjugates

3 Modification of Nucleic Acids and Oligonucleotides

4 Creating Specific Functionalities

5 Blocking or Protecting Groups

Chapter 3. The Reactions of Bioconjugation

1 Amine Reactions

2 Thiol Reactions

3 Carboxylate Reactions

4 Hydroxyl Reactions

5 Aldehyde and Ketone Reactions

6 Active Hydrogen Reactions

7 PhotoChemical Reactions

8 Cycloaddition Reactions

Chapter 4. Zero-Length Crosslinkers

1 Carbodiimides

2 Woodward’s Reagent K

3 N, N′-Carbonyl Diimidazole

4 Schiff Base Formation and Reductive Amination

Chapter 5. Homobifunctional Crosslinkers

1 Homobifunctional NHS Esters

2 Homobifunctional Imidoesters

3 Homobifunctional Sulfhydryl Reactive Crosslinkers

4 Difluorobenzene Derivatives

5 Homobifunctional Photoreactive Crosslinkers

6 Homobifunctional Aldehydes

7 BIS-Epoxides

8 Homobifunctional Hydrazides

9 BIS-Diazonium Derivatives

10 BIS-Alkylhalides

Chapter 6. Heterobifunctional Crosslinkers

1 Amine-Reactive and Sulfhydryl-Reactive Crosslinkers

2 Carbonyl Reactive and Sulfhydryl Reactive Crosslinkers

3 Amine-Reactive and Photoreactive Crosslinkers

4 Sulf


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© 2013
Academic Press
Electronic ISBN:
Print ISBN:

About the author


"Most of the chapters address specific systems and reagents such as (strept) avidin-biotin, silane coupling, buckyballs & fullerenes, antibodies, liposome conjugates, and modified nucleotides—as well as a few chapters on observation techniques such as fluorescent probes and isotopic labeling. Also new since the last edition is a chapter addressing immobilization in chromatography.", February 2014

"This is an incomparable and essential guide for any scientist involved in conjugating biologically active molecules. The new edition does not disappoint with its liberal use of color (especially in reaction schemes), expanded pages, and newly added chapters. For those interested in bioconjugate chemistry, you may soon find that this is the only book on your research library shelf!" Rating: 5 Stars, February 2014