Biochemistry LabFax

Biochemistry LabFax

1st Edition - August 3, 1993

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  • Editor: B. Hames
  • eBook ISBN: 9781483294445

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Description

LABFAX volumes are purpose-designed data reference books for practising scientists. Each book presents key information for a major subject in one place and so saves hours of searching. It does not simply collect together data which are already available in catalogues since these are often incomplete and can contain conflicting information. Rather, the authors and editors of each LABFAX volume have searched the original literature for the accurate data which they know the specialist needs.Biochemistry LabFax is a detailed compendium of essential information - on radioisotopes, enzymes, proteins, nucleic acids, lipids, plus data on selected techniques such as chromatography, electrophoresis, etc. - needed on an almost daily basis by researchers in any area of biochemistry. In addition, the book acts as a gateway to other sources for information more specific than can be covered in a volume of this size.

Table of Contents


  • Contributors

    Abbreviations

    1. Buffers, Chelating Agents And Denaturants

    Buffers

    Introduction

    Goods' Buffers

    Preparation of Buffers

    Counter Ions

    Effects of Temperature and Concentration

    Biological Compatibility and Chemical Reactivity

    Preparation of Phosphate Buffers

    Preparation of Acetate Buffers

    Buffered Salines, Balanced Salts and Osmotic Supports

    Chelating Agents

    Denaturing Agents for Proteins

    Mechanisms of Denaturing Agents

    Mixing Denaturants

    Characteristics of Denaturants and Precipitants

    Thiol Reagents

    Detergents

    Properties of Acids, Bases, Salts and Organic Solvents

    Amino Acids

    Ammonium Sulfate Precipitation Chart for Proteins

    References

    2. Radioisotopes in Biochemistry

    Definitions

    Units of Radioactivity

    Specific Activity

    Electron Volt (eV, or MeV=106 eV)

    Gray

    Dose Equivalent Man (Sievert)

    Radioisotopes Used in Biochemistry

    Radioactive Decay Correction

    Autoradiography

    Scintillation Counting

    Cerenkov Counting

    Counting of Finely Dispersed or Solvent-Soluble Substances

    Counting of Aqueous Samples

    Gamma Counting

    Use of Radioisotopes as Tracers

    Radiological Protection

    Shielding for β-Particles and γ-Rays

    Methods for Decontaminating Laboratories

    References

    Further Reading

    3. Chromatographic Fractional Media

    Introduction

    Media for Size Exclusion

    Media for Ion-Exchange Separations

    Media for Reversed-Phase Separations

    Media for Affinity Chromatography

    Manufacturers and Suppliers of Chromatography Column Support Materials

    References

    Further Reading

    4. Electrophoresis of Proteins and Nucleic Acids

    Proteins

    Separations on Denaturing Gels

    Separations on Non-Denaturing Gels

    Separations of Proteins by Isoelectric Focusing

    Two-Dimensional Gel Electrophoresis

    Marker Proteins

    Staining Protein Gels

    Nucleic Acids

    Gels for Separating Nucleic Acids and Nucleoproteins

    Markers for Nucleic Acids

    Staining Nucleic Acid Gels

    Methods Manuals on Electrophoresis

    5. General Centrifugation Data

    Calculation of Centrifugal Force

    Applications of Centrifuge Rotors

    Calculation of k-Factors of Rotors and Pelleting Times

    Derating Rotors for Use with Dense Solutions

    Properties of Centrifuge Tubes and Bottles

    Sterilization and Disinfection Procedures

    Sterilization Techniques

    Biological Disinfection

    Equations Relating the Refractive Index to the Density of Solutions

    Properties of Sucrose Solutions

    6. Enzymology

    Introduction

    Enzyme Kinetics

    Enzyme Kinetics of Single Substrate Reactions

    Sigmoidal Kinetics and Allosteric Enzymes

    Enzyme Kinetics of Bisubstrate Reactions

    Enzyme Inhibitors

    Reversible Inhibition

    Irreversible Inhibition

    Effects of pH on Enzymes

    Coenzymes — Structure and Functions

    Enzyme Assays

    Handling and Storage of Enzymes and Coenzymes

    References

    Further Reading

    7. Hydrolytic Enzymes

    Introduction

    Inhibition of Nucleases and Proteases

    Deoxyribonucleases

    Ribonucleases

    Proteases

    Carbohydrases

    Naturally Occurring or Physiological Inhibitors

    References

    8. Characteristics of Selected Proteins

    References

    9. Glycoproteins and Protein Glycosyution

    Introduction

    Occurrence of Glycosylated Proteins in Nature

    Range and Types of Glycosylated Proteins

    Structural Features of Glycosylated Proteins

    Linkage of Carbohydrate to Proteins

    Oligosaccharide Structures

    Enzymic Glycosylation — Synthesis of Sequence

    Monosaccharide Transport, Interconversion and Activation

    Glycosyltransferases

    Εxamples of Functional Roles for Protein Glycosylation

    References

    10. Chemical and Post-Translational Modification of Proteins

    Chemical Modification of Proteins

    Enzyme-Catalyzed Covalent Modification Reactions

    Introduction

    Influence of Modification

    Characteristics of Modification Reactions

    Physiological Role of the Modification

    Reversibility of Reactions

    Summary

    References

    11. Nucleic Acids and their Components

    Nucleosides and Nucleotides

    Basic Structures

    Nucleotides as Acids

    Optical Density of Bases, Nucleosides and Nucleotides

    Nucleotide-Derived Compounds

    Selected Nucleotide Analogs

    DNA of Selected Organisms

    The Genetic Code

    Abbreviations of Amino Acids

    Assays for Nucleic Acids

    References

    12. Lipids

    Introduction

    Structure and Characteristics of Acyl Lipids

    Fatty Acids

    Neutral Acyl Lipids — Wax Esters, Acylglycerols and Glycerol Ethers

    Glycerophospholipids

    Glyceroglycolipids

    Sphingolipids

    Structure and Distribution of Terpenoid Constituents of Membranes

    Sterols

    Chlorophylls and Carotenoids

    Composition and Distribution of Lipids in Membranes

    Composition of Membrane Lipids

    Fatty Acid Composition of Membrane Lipids

    Structure and Properties of Bioactive Lipids

    Eicosanoids

    Platelet Activating Factor

    Diacylglycerol

    Steroid Hormones

    Structure and Composition of Bile Acids and Bile Salts

    Chemical Abstracts Registry Numbers of Lipids Cited

    References

    13. Carbohydrates and Sugars

    Introduction

    Structures and Characteristics of Monomeric Carbohydrates

    Classification of Monosaccharides

    Distribution and Properties of some Monosaccharides

    Stereoisomerism

    Oligosaccharides

    Classification

    Distribution and Properties

    Structures and Characteristics of Polysaccharides

    Classification

    Plant Polysaccharides

    Bacterial Polysaccharides

    Animal Polysaccharides

    DNA, RNA, Nucleosides and Nucleotides

    Other Saccharide Derivatives

    Further Reading

    14. Safety and the Disposal of Toxic and Infectious Materials

    Protection from Chemical Hazards

    Risk and Safety Classification Systems

    Disposal of Toxic and Infectious Materials

    Disposal of Toxic Materials

    Disposal of Biohazardous Materials

    References

    15. Sources of Further Biochemical Data

    Reference Books

    Databases

    Nucleic Acid Sequence Databases

    Contact Addresses

    16. Atomic Weights and Mathematical Formulae

    Atomic Weights

    Mathematical Formulae

    Lengths, Areas and Volumes in some Common Geometric Figures

    Inter-Relations of Sides and Angles in a Plane Triangle

    Trigonometrical Data

    Mathematical Series

    Mathematical Constants

    Standard Equations

    Differentials and Integrals

    Index

Product details

  • No. of pages: 361
  • Language: English
  • Copyright: © Academic Press 1993
  • Published: August 3, 1993
  • Imprint: Academic Press
  • eBook ISBN: 9781483294445

About the Series Editor

B. Hames

Affiliations and Expertise

Consultant Microbiologist, Freeman Hospital, Newcastle upon Tyne, UK

About the Series Volume Editors

J. Chambers

Affiliations and Expertise

1487 West 5th Avenue, Columbus, Ohio, U.S.A.

D. Rickwood

Affiliations and Expertise

University of Essex, Wivenhoe Park, Colchester

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