Biochemical Pathways and Environmental Responses in Plants, Part A

Biochemical Pathways and Environmental Responses in Plants, Part A

1st Edition - February 1, 2023

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  • Editor: Joseph Jez
  • Hardcover ISBN: 9780323955614

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Description

Biochemical Pathways and Environmental Responses in Plants, Part A, Volume 681 in the Methods in Enzymology series highlights new advances in the field with this new volume presenting interesting chapters on topics such as Structure, function, and engineering of plant polyketide synthases, A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation, Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay, An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry, and much more. Other chapters cover Biochemical characterization of plant aromatic aminotransferases, Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites, A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions, Plant metacaspase: an example of microcrystal structure determination and analysis, Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners, Dirigent Protein Family Function and Structure, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology series
  • Includes the latest information on Biochemical pathways and environmental responses in plants

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Structure, function, and engineering of plant polyketide synthases
    Ikuro Abe
    2. A sensitive LC-MS/MS assay for enzymatic characterization of methylthioalkylmalate synthase involved in glucosinolate side-chain elongation
    N Bisht
    3. Assaying formate-tetrahydrofolate ligase with monoglutamylated and polyglutamylated substrates using a fluorescence-HPLC based assay
    Sanja Roje
    4. An Approach to Nearest Neighbor Analysis of Pigmented Protein Complexes by Using Chemical Crosslinking in Combination with Mass Spectrometry
    Haijun Liu
    5. Biochemical characterization of plant aromatic aminotransferases
    Hiroshi Maeda
    6. Functional Analysis of Phosphoethanolamine N-methyltransferase (PMT) in Plants and Parasites
    Soon Goo Lee
    7. A structure-guided computational screening approach for predicting plant enzyme-metabolite interactions
    Cynthia Holland
    8. Plant metacaspase: an example of microcrystal structure determination and analysis
    Qun Liu
    9. Biocatalytic system for comparative assessment of functional association of cytochrome P450 monooxygenases with their redox partners
    Chang-Jun Liu
    10. Dirigent Protein Family Function and Structure
    Norman G. Lewis
    11. Lignin/Lignan Biosynthesis Structure/Function
    Norman G. Lewis
    12. Discovery and characterization of BAHD acylsugar acyltransferases
    Robert L. Last
    13. Near-real time determination of BAHD acyl-coenzyme A transferase reaction rates and kinetic parameters using Ellman's reagent
    Michael Sullivan
    14. Quantification of N-terminal protein acetyltransferase activity in vitro and in planta
    Markus Wirtz
    15. Computational and biochemical methods to measure the activity of carboxysomes and protein organelles in vivo
    Jeffrey Cameron
    16. Analysis of Plant Alkaloid-Protein Interactions Using Differential Scanning Fluorimetry and Isothermal Titration Calorimetry
    Peter Facchini
    17. A metabolomics strategy for plant specialized metabolites using liquid chromatography-mass spectrometry
    Kazuki Saito and Tetsuya Mori
    18. Isoprene measurements to assess the methylerythritol pathway
    Thomas D. Sharkey
    19. Analysis of isoprenoid alcohol phosphates by liquid chromatorgraphy-mass spectrometry
    Peter Dörmann and Katharina Gutbrod
    20. Using targeted metabolomics to elucidate the indole auxin network in plants
    Jerry D. Cohen and Adrian D. Hegeman
    21. Metabolite fingerprinting: A powerful metabolomics approach for marker identification and functional gene annotation
    Ivo Feussner
    22. Ex vivo metabolomics, an hypothesis-free approach to identify native substrate(s) of orphan enzymes
    Kirstin Feussner
    23. Using stable isotopes and LC-MS for precursor of origin determination in plants
    Clint Chapple
    24. Measurement of flux through sulfate assimilation using [35S]sulfate
    Stanislav Kopriva
    25. Assessment of the activity of sulfate uptake and assimilation in plants
    Rüdiger Hell
    26. Comparison of TLC, HPLC, and direct infusion mass spectrometry methods for the identification and quantification of diacylglycerol molecular species
    Philip Bates
    27. Plant sphingolipid analysis
    Jennifer E. Markham
    28. Strategies to study the metabolic origins of plant specialized metabolites: the 1,4-naphthoquinones in Lithospermum erythrorhizon
    Joshua R. Widhalm
    29. Controlled environments for highly reproducible cannabis ‘omics’ studies
    Bernd Markus Lange
    30. Multi-platform approaches for the comprehensive analysis of aroma volatiles in cannabis
    Bernd Markus Lange
    31. Systems and strategies for plant protein expression
    Thomas D. Niehaus
    32. Programmable Chemical Actuator Control of Soluble and Membrane-Bound Enzymatic Catalysis
    Katrina Cornish
    33. CRISPR-Cas mediated genome engineering of cyanobacteria
    Himadri B. Pakrasi
    34. Plant Seed Proteome Analysis
    Hari B. Krishnan
    35. Proteomics and phosphoproteomics of C3 to CAM transition in the common ice plant
    Sixue Chen
    36. Identification of MPK4 interactome using TurboID proteomics
    Sixue Chen
    37. A Three-in-One Method for High Throughput Plant Multiomics
    Sixue Chen
    38. Assessing simultaneous reversible cysteine oxidation and global abundance in plant tissues
    Leslie M. Hicks
    39. Analysis of plant flooding response
    Simon Gilroy
    40. Imaging systemic calcium response and its molecular dissection using virus-induced gene silencing
    Simon Gilroy
    41. Studying the dynamics of transcriptional complex formation as a function of temperature using small angle x-ray scattering and in vitro assays
    Chloe Zubieta
    42. Isolation of novel chemical components and their target plant proteins in metal stress conditions
    Ryoung Shin
    43. Inhibition of insect Na+/K+ ATPases by plant-derived cardiac glycosides
    Georg Petschenka Jander
    44. High-throughput phenotyping and the use of computer vision to unravel novel phenotypes associated with plant nutrition
    David Mendoza-Cozatl
    45. Detection of protein persulfidation in plants by the dimedone switch method
    Luis C. Romero and Cecilia Gotor
    46. Using Hyper as a Molecular Probe to Visualize Hydrogen Peroxide in Living Plant Cells: An Updated Method
    Luis Cárdenas
    47. Genetically encoded sensors for monitoring the spatiotemporal dynamics of glucose and sucrose
    Wolf B. Frommer and Nora Zoellner
    48. Acetylene Reduction Assay for Nitrogenase Activity
    Senthil Subramanian
    49. Monitoring lipid-protein interactions in planta using Förster resonance energy transfer
    Xuemin (Sam) Wang
    50. Modification of G-protein biochemistry and its effect on plant/environment interaction.
    Sona Pandey
    51. RNA-seq analysis of alternative pre-mRNA splicing mediated by photoreceptors in Physcomitrium patens
    Shih-Long Tu

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2023
  • Published: February 1, 2023
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323955614

About the Serial Volume Editor

Joseph Jez

Prof. Jez received his B.S. in biochemistry from Penn State University (1992), a Ph.D. in biochemistry & molecular biophysics from the University of Pennsylvania (1998), and was an NIH-NRSA postdoctoral fellow at the Salk Institute for Biological Studies (1998-2001). After working as a research scientist at Kosan Biosciences (2001-2002), he started his research group at the Donald Danforth Plant Science Center and moved to the Department of Biology at Washington University in 2008. He has authored more than 160 papers and received a Presidential Early Career Award for Scientists and Engineers (2005), the Phytochemical Society of North America's Arthur Neish Young Investigator Award (2007), and a Fulbright Senior Specialist Award (2012) and was named as Howard Hughes Medical Institute Professor in 2014. Research in the Jez lab seeks to understand how environmental changes re-model biochemical pathways in plants at the molecular, cellular, and organism levels with the aim of engineering these systems to address agricultural and environmental problems. Current work in the lab employs a combination of structural biology, protein chemistry, and plant biology. He also launched the Biotech Explorers Program, which aims to introduce undergraduates to how teams in science can tackle real world problems using cross-disciplinary approaches.

Affiliations and Expertise

Department of Biology, Washington University in St. Louis, USA

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