Biochemical Aspects of Reactions on Solid Supports

Biochemical Aspects of Reactions on Solid Supports

1st Edition - January 1, 1971

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  • Editor: George Stark
  • eBook ISBN: 9780323159234

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Description

Biochemical Aspects of Reactions on Solid Supports focuses on water-soluble enzyme derivatives and their possible use as heterogeneous specific catalysts in research and industry. It discusses the four fundamental methods used for the preparation of water-insoluble derivatives of biologically active proteins. Organized into five chapters, the book starts by reviewing the methods available for the immobilization of proteins, which includes enzymes adsorption, occlusion in cross-linked polymeric matrices, covalent binding to water-insoluble carriers, and intermolecular cross-linking. It then discusses the principle of affinity chromatography, which can be applied to a wide range of macromolecular-ligand systems. Other chapters consider the developments in the use of solid support and reagents in peptide synthesis. In addition, the book discusses the various methods used in the production of cation-exchange resins for amino acid chromatography and water softening. This book is a valuable resource for biochemists, chemical biologists, scientists, students, and researchers whose interests span a variety of fields.

Table of Contents


  • List of Contributors

    Preface

    Chapter 1. Water-Insoluble Enzyme Derivatives and Artificial Enzyme Membranes

    I. Introduction

    II. Preparation of Water-Insoluble Enzyme Derivatives

    III. Stability of Water-Insoluble Enzyme Derivatives

    IV. Kinetic Behavior of Particulate Immobilized Enzyme Systems

    V. Enzyme Columns

    VI. Enzyme Membranes

    VII. Applications

    References

    Chapter 2. Selective Adsorbents Based on Biochemical Specificity

    I. Introduction

    II. Water-Insoluble Carriers

    III. Selection of the Ligand

    IV. Covalent Linking Reactions

    V. Conditions for Chromatography on Affinity Columns

    VI. Importance of Anchoring Arms

    VII. Specific Adsorbents for Protein Purification

    VIII. Specific Nucleic Acid Adsorbents

    IX. Isolation of Cells, Receptor Structures, and Other Particulate Cell Structures

    References

    Chapter 3. Solid Phase Synthesis: The Use of Solid Supports and Insoluble Reagents in Peptide Synthesis

    I. Introduction

    II. Polymeric Carboxyl Protection

    III. Polymeric Amino Protection

    IV. Polymeric Side-Chain Protection

    V. Polymeric Coupling Reagents

    VI. Effects of Physical Properties of Polymeric Supports

    VII. Automation

    VIII. Similar Development in Conventional Synthesis

    IX. Difficulties in Analysis

    X. Results

    XI. Conclusions and Summary

    References

    Chapter 4. Sequential Degradation of Peptides Using Solid Supports

    I. Introduction

    II. Degradation of Peptides Attached to Solid Supports

    III. Degradation of Peptides with an Insoluble Reagent

    IV. Properties of Resins Important for Peptide Degradations

    References

    Chapter 5. Preparation of Cross-Linked Polystyrenes and Their Derivatives for Use as Solid Supports or Insoluble Reagents

    I. Introduction

    II. Types of Polystyrene and Their Properties

    III. The Preparation of Spherical Styrene-DVB Copolymers

    IV. Chemical Reactions of Styrene-Divinylbenzene Copolymers

    References

    Author Index

    Subject Index


Product details

  • No. of pages: 246
  • Language: English
  • Copyright: © Academic Press 1971
  • Published: January 1, 1971
  • Imprint: Academic Press
  • eBook ISBN: 9780323159234

About the Editor

George Stark

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