Biochemical Aspects of Reactions on Solid Supports - 1st Edition - ISBN: 9780126639506, 9780323159234

Biochemical Aspects of Reactions on Solid Supports

1st Edition

Editors: George Stark
eBook ISBN: 9780323159234
Imprint: Academic Press
Published Date: 1st January 1971
Page Count: 246
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Biochemical Aspects of Reactions on Solid Supports focuses on water-soluble enzyme derivatives and their possible use as heterogeneous specific catalysts in research and industry. It discusses the four fundamental methods used for the preparation of water-insoluble derivatives of biologically active proteins. Organized into five chapters, the book starts by reviewing the methods available for the immobilization of proteins, which includes enzymes adsorption, occlusion in cross-linked polymeric matrices, covalent binding to water-insoluble carriers, and intermolecular cross-linking. It then discusses the principle of affinity chromatography, which can be applied to a wide range of macromolecular-ligand systems. Other chapters consider the developments in the use of solid support and reagents in peptide synthesis. In addition, the book discusses the various methods used in the production of cation-exchange resins for amino acid chromatography and water softening. This book is a valuable resource for biochemists, chemical biologists, scientists, students, and researchers whose interests span a variety of fields.

Table of Contents

List of Contributors Preface Chapter 1. Water-Insoluble Enzyme Derivatives and Artificial Enzyme Membranes I. Introduction II. Preparation of Water-Insoluble Enzyme Derivatives III. Stability of Water-Insoluble Enzyme Derivatives IV. Kinetic Behavior of Particulate Immobilized Enzyme Systems V. Enzyme Columns VI. Enzyme Membranes VII. Applications References Chapter 2. Selective Adsorbents Based on Biochemical Specificity I. Introduction II. Water-Insoluble Carriers III. Selection of the Ligand IV. Covalent Linking Reactions V. Conditions for Chromatography on Affinity Columns VI. Importance of Anchoring Arms VII. Specific Adsorbents for Protein Purification VIII. Specific Nucleic Acid Adsorbents IX. Isolation of Cells, Receptor Structures, and Other Particulate Cell Structures References Chapter 3. Solid Phase Synthesis: The Use of Solid Supports and Insoluble Reagents in Peptide Synthesis I. Introduction II. Polymeric Carboxyl Protection III. Polymeric Amino Protection IV. Polymeric Side-Chain Protection V. Polymeric Coupling Reagents VI. Effects of Physical Properties of Polymeric Supports VII. Automation VIII. Similar Development in Conventional Synthesis IX. Difficulties in Analysis X. Results XI. Conclusions and Summary References Chapter 4. Sequential Degradation of Peptides Using Solid Supports I. Introduction II. Degradation of Peptides Attached to Solid Supports III. Degradation of Peptides with an Insoluble Reagent IV. Properties of Resins Important for Peptide Degradations References Chapter 5. Preparation of Cross-Linked Polystyrenes and Their Derivatives for Use as Solid Supports or Insoluble Reagents I. Introduction II. Types of Polystyrene and Their Properties III. The Preparation of Spherical Styrene-DVB Copolymers


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© Academic Press 1971
Academic Press
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About the Editor

George Stark

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