Biochemical Aspects of Reactions on Solid Supports

1st Edition - January 1, 1971
Editor: George Stark
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 5 9 2 3 - 4

Biochemical Aspects of Reactions on Solid Supports focuses on water-soluble enzyme derivatives and their possible use as heterogeneous specific catalysts in research and industry.… Read more

Purchase options

LIMITED OFFER

Save 50% on book bundles

Immediately download your ebook while waiting for your print delivery. No promo code is needed.

Institutional subscription on ScienceDirect

Request a sales quote

Biochemical Aspects of Reactions on Solid Supports focuses on water-soluble enzyme derivatives and their possible use as heterogeneous specific catalysts in research and industry. It discusses the four fundamental methods used for the preparation of water-insoluble derivatives of biologically active proteins. Organized into five chapters, the book starts by reviewing the methods available for the immobilization of proteins, which includes enzymes adsorption, occlusion in cross-linked polymeric matrices, covalent binding to water-insoluble carriers, and intermolecular cross-linking. It then discusses the principle of affinity chromatography, which can be applied to a wide range of macromolecular-ligand systems. Other chapters consider the developments in the use of solid support and reagents in peptide synthesis. In addition, the book discusses the various methods used in the production of cation-exchange resins for amino acid chromatography and water softening. This book is a valuable resource for biochemists, chemical biologists, scientists, students, and researchers whose interests span a variety of fields.

List of Contributors

Preface

Chapter 1. Water-Insoluble Enzyme Derivatives and Artificial Enzyme Membranes

I. Introduction

II. Preparation of Water-Insoluble Enzyme Derivatives

III. Stability of Water-Insoluble Enzyme Derivatives

IV. Kinetic Behavior of Particulate Immobilized Enzyme Systems

V. Enzyme Columns

VI. Enzyme Membranes

VII. Applications

References

Chapter 2. Selective Adsorbents Based on Biochemical Specificity

I. Introduction

II. Water-Insoluble Carriers

III. Selection of the Ligand

IV. Covalent Linking Reactions

V. Conditions for Chromatography on Affinity Columns

VI. Importance of Anchoring Arms

VII. Specific Adsorbents for Protein Purification

VIII. Specific Nucleic Acid Adsorbents

IX. Isolation of Cells, Receptor Structures, and Other Particulate Cell Structures

References

Chapter 3. Solid Phase Synthesis: The Use of Solid Supports and Insoluble Reagents in Peptide Synthesis

I. Introduction

II. Polymeric Carboxyl Protection

III. Polymeric Amino Protection

IV. Polymeric Side-Chain Protection

V. Polymeric Coupling Reagents

VI. Effects of Physical Properties of Polymeric Supports

VII. Automation

VIII. Similar Development in Conventional Synthesis

IX. Difficulties in Analysis

X. Results

XI. Conclusions and Summary

References

Chapter 4. Sequential Degradation of Peptides Using Solid Supports

I. Introduction

II. Degradation of Peptides Attached to Solid Supports

III. Degradation of Peptides with an Insoluble Reagent

IV. Properties of Resins Important for Peptide Degradations

References

Chapter 5. Preparation of Cross-Linked Polystyrenes and Their Derivatives for Use as Solid Supports or Insoluble Reagents

I. Introduction

II. Types of Polystyrene and Their Properties

III. The Preparation of Spherical Styrene-DVB Copolymers

IV. Chemical Reactions of Styrene-Divinylbenzene Copolymers

References

Author Index

Subject Index