Autoradiography at the Cellular Level - 2nd Edition - ISBN: 9781483167398, 9781483194875

Autoradiography at the Cellular Level

2nd Edition

Physical Techniques in Biological Research

Authors: Brigitte Schultze
Editors: Arthur W. Pollister
eBook ISBN: 9781483194875
Imprint: Academic Press
Published Date: 1st January 1969
Page Count: 212
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Description

Physical Techniques in Biological Research, Second Edition, Volume III, Part B: Autoradiography at the Cellular Level focuses on high resolution autoradiography with tritium.

This book reviews the different autoradiographic techniques and difficulties in theoretical principles needed for the interpretation of autoradiograms. The topics include the characteristics of the different types of radiation; autoradiographic resolution; techniques for applying photographic emulsions; and autoradiography of water-soluble substances. The application of different isotopes; general aspects of the use of thymidine; physiological RNA synthesis; and RNA synthesis under pathological conditions are also elaborated. This text likewise covers the protein synthesis under normal conditions; electron microscopic autoradiography; application of electron microscopic autoradiography; and autoradiographic methods in cytochemistry.

This publication is a good reference for students, researchers, and clinicians conducting work on the use of tritiated compounds in high resolution autoradiography.

Table of Contents


Preface


Preface to Second Edition of Volume III


1. General and Theoretical Aspects of Autoradiography


I. Characteristics of the Different Types of Radiation


II. Autoradiographic Emulsions


III. Photographic Process


IV. Autoradiographic Resolution


V. Dosage of Isotopes


VI. Background and Artifacts


VII. Quantitative Autoradiography


2. Autoradiographic Techniques


I. Preparation of Tissue


II. Techniques for Applying Photographic Emulsions


III. Exposure


IV. Development and Fixation


V. Staining


VI. Autoradiography of Water-Soluble Substances


3. Application of Different Isotopes


I. Iodine-131 and Iodine-125


II. Sulfur-35


III. Phosphorus-32


IV. Carbon-14


V. Iron-59


VI. Calcium-45


VII. Strontium-90, Strontium-89, and Strontium-85


VIII. Other Isotopes


IX. Water-Soluble Cations and Anions


4. DNA Synthesis


I. General Aspects of the Use of Thymidine


II. Autoradiographic Studies of the Cellular DNA Synthesis with Labeled Thymidine


5. RNA Synthesis


I. General Aspects of Autoradiographic Studies on RNA Synthesis


II. Physiological RNA Synthesis


III. RNA Synthesis Under Pathological Conditions


IV. RNA Synthesis After X-Ray Irradiation


6. Protein Synthesis


I. Correlation Between Amino Acid Incorporation Rate and the Specific Activity of the Free Amino Acid


II. Protein Synthesis Under Normal Conditions


III. Protein Synthesis Under Special Conditions


7 . Lipid Synthesis


Text


8. Electron Microscopic Autoradiography


I. General Considerations of the Met

Details

No. of pages:
212
Language:
English
Copyright:
© Academic Press 1969
Published:
Imprint:
Academic Press
eBook ISBN:
9781483194875

About the Author

Brigitte Schultze

About the Editor

Arthur W. Pollister

Ratings and Reviews