This third volume in the series covers such topics as anaesthetics, cannulation and injection techniques, and surgery. The book will be invaluable to fisheries scientists, aquaculturists, and animal biochemists, physiologists and endocrinologists; it will provide researchers and students with a pertinent information source from theoretical and experimental angles.

Table of Contents

Preface. Contributors. Abbreviations. Anaesthetics (G.K. Iwama, P.A. Ackerman). Cannulation techniques (M. Axelsson, R. Fritsche). Surgery: selective islet removal and total pancreatectomy (A. Epple, B. Nibbio). Hypophysectomy of fish (R.S. Nishioka). Surgical extirpation of the Stannius corpuscles: stanniectomy (J.C. Fenwick). Microsurgery: gonadectomy (E. McLean). Hepatectomy (Y. Inui). Injection techniques (S.F. Perry, S.G. Reid). Techniques for recording from electric organs (H.H. Zakon). Intact and demembranated muscle fibres (J.D. Altringham, I.A. Johnston). Isolated perfused fish hearts (W.R. Driedzic, J.R. Bailey). Methods for assessing kidney and urinary bladder function in fish (C.M. Wood, M.L. Patrick). Cell lines: availability, propagation and isolation (N.C. Bols, L.E.J. Lee). Extracorporeal circulation (S. Thomas). Perifusion of pancreatic islets (S.L. Milgram, J.K. McDonald, B.D. Noe). Perfusion of pancreatic endocrine tissue of teleost fish (P. Ronner). The isolated rete (E.A. Rasio, M. Bendayan, C.A. Goresky). Teleost pituitary cells: Isolation, culture and use (J.P. Chang, R.M. Jobin). Photophores: the analysis of bioluminescent systems (J.-F. Rees, E.M. Thompson). Opercular membranes and skin (S.D. McCormick). Isolation of gill cells (P.M. Verbost, G. Flik, H. Cook). Testes cells: isolation and culture (M. Loir, P. Sourdaine). Primary culture of rainbow trout corpuscles of Stannius (B. Gellersen, G.F. Wagner). Isolated cardiac myocytes (C.L. Milligan). Oogenesis (H. Kagawa). Adipose tissue (M.A. Sheridan, J.S. Harmon). Enzymes of lipogenesis (H. Segner, R. Böhm). Isolation and culture of head kidney macrophages (R.A. Garduño, W.W. Kay). In vitro culture of fish leukocytes (N.W. Miller, E. Churchill McKinney). Hepatocytes: isolation, maintenance and utilization (T.P. Mommsen, T.W. Moon, P.J. Walsh). Organ culture of fish tissues (P.A. Janssens, J.A. Grigg). Hepatocyte perifusion (C. Ottolenghi et al.). Intracel


© 1994
Elsevier Science
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@from:H. Goos @qu:...presents an overwhelming amount of very useful technical information and it will be a really useful guide not only for all researchers in fish physiology. But also for aquaculturists, who will find some useful techniques, such as blood sampling, anaesthetics and operation techniques. @source:Aquaculture