Anaerobic Bacteriology

Anaerobic Bacteriology

Clinical and Laboratory Practice

3rd Edition - January 1, 1977

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  • Author: A. Trevor Willis
  • eBook ISBN: 9781483191850

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Anaerobic Bacteriology: Clinical and Laboratory Practice, Third edition discusses the importance of the non-sporing anaerobic bacteria as a significant cause of infection in man. This edition updates the anaerobic methodology, systematics, and ecological and pathogenetic associations of the non-sporing anaerobes. The descriptive bacteriology of the non-clostridial anaerobes and clinical syndromes produced by them in man are also considered. Other topics discussed include the anaerobic jar, inoculation of media, and antibiotic susceptibility testing of anaerobes. The histotoxic clostridia of infected wounds, anaerobic cocci, and infections related to the gastrointestinal tract are also elaborated. This text likewise covers the uterine gas gangrenous infections and other clostridial infections. This book is a good source for medical practitioners, clinicians, and medical students concerned with anaerobic bacteria.

Table of Contents

  • 1. Methods of Growing Anaerobes

    The Anaerobic Jar

    Other Methods of Obtaining an Oxygen-Free Atmosphere

    Pre-Reduced Anaerobically Sterilized Roll Tube Technique

    Anaerobic Cabinets and Glove Boxes

    Shake Cultures and Fluid Cultures

    Reducing Agents



    2. Media

    Inoculation of Media

    Preparation of Media

    Some Medium Additives

    Cooked Meat Medium

    VL (Viande—Levure) Broth

    Reinforced Clostridial Medium

    Columbia Agar Base

    Fresh Blood Agar

    Heated Blood (Chocolate) Agar

    Benzidine Heated Blood Agar

    Egg Yolk Agar and Human Serum Agar

    Milk Medium and Litmus Milk

    Milk Agar

    Casein Agar

    Gelatin Agar

    Deoxyribonuclease Agar (DNAse Agar)

    Tributyrin Agar

    Concentrated (Firm) Agar

    Media for Sporulation of Cl. Perfringens

    Fermentation Media

    Bile Broth

    Hydrogen Sulphide Production

    Indole Production

    Nitrate Reduction

    Catalase Production

    Urea Medium

    The API Microsystem for Anaerobes

    Selective Agents

    Selective Media

    Media for Anaerobic Blood Culture

    Stock Cultures of Anaerobes


    3. Examination and Identification of Anaerobes

    Morphology and Staining

    Colonial Appearance

    Cultural Characteristics

    Antibiotic Disc Resistance

    Growth Inhibition and Stimulation

    Antibiotic Susceptibility Testing of Anaerobes

    Animal Inoculation and Protection Tests

    Serological and Toxicological Aspects of Anaerobes

    Gas Liquid Chromatography


    4. Characteristics of The Pathogenic and Related Clostridia



    Clostridium Tetani

    Clostridium Botulinum

    The Histotoxic Clostridia of Infected Wounds

    Clostridium Perfringens

    Clostridium Novyi

    Clostridium Septicum and Clostridium Chauvoei

    Clostridium Histolyticum

    Clostridium Tertium

    Clostridium Sporogenes

    Clostridium Bifermentans and Clostridium Sordellii

    Clostridium Fallax

    Clostridium Cochlearium

    Clostridium Paraputrificwn

    Clostridium Lentoputrescens

    Clostridium Cadaveris

    Clostridium Sphenoides

    Clostridium Carnis

    Clostridium Difficile

    Clostridium Subterminale

    Clostridium Barati


    5. The Non-Clostridial Anaerobes



    Actinomyces and Arachnia



    The Anaerobic Cocci

    Veillonella Alcalescens and Veillonella Parvula




    6. Infections Due To Non-Clostridial Anaerobes

    Obstetrical and Gynaecological Infections

    Other Genitourinary Infections

    Infections Related to the Gastrointestinal Tract

    Infections of the Respiratory Tract

    Intracranial Suppurative Disease

    Bone and Joint Infections

    Superficial and Soft Tissue Infections



    Treatment of Bacteroides and Related Infections

    Diagnosis of Non-Clostridial Anaerobic Infections



    7. Clostridial Infections

    Wound Infections


    Gas Gangrene (Clostridial Myonecrosis)

    Uterine Gas Gangrenous Infections


    Cl Perfringens Food Poisoning

    Enteritis Necroticans (Necrotizing Jejunitis)

    Necrotizing Colitis

    Other Clostridial Infections



Product details

  • No. of pages: 370
  • Language: English
  • Copyright: © Butterworth-Heinemann 1977
  • Published: January 1, 1977
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483191850

About the Author

A. Trevor Willis

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