Amine Oxidases in Clinical Research discusses the functions of amine oxidases in the rapid development of medical and biological research. Composed of 13 chapters, the book focuses on amine oxidases, including their key role in the metabolism of biogenic amines and other nitrogenous compounds in diseases and human health. The book considers primarily the enzymes denoted as EC 188.8.131.52 (flavin amine oxidases or monoamine oxidases) and EC 184.108.40.206 (copper-containing amine oxidase or diamine oxidases). To measure the estimates of amine oxidase activity, several methods and a number of requirements are presented. A description of the distribution and intracellular localization of amine oxidase follows. The methods of isolation and purification of amine oxides from different sources and the results of these various methods are explained. The text also considers the properties of the purified amine oxidases: the different methods of isolation and purification; storage conditions; and analytical techniques. Care is given when examining amine oxidases, because these may exhibit considerable differences in substrate specificity even if their physicochemical properties are similar. Inhibition of amine oxidase activity by unspecific and specific inhibitions is further discussed, including the biological functions and known effects of hormones on the biosynthesis and catabolism of the amine oxidase. Finally, the possible pathogenetic and/or diagnostic significance of the amine oxidases, as well as the corrective approaches seen in pathological conditions and in clinical medicine, are explained. Written as a handbook for the medically oriented investigators, biologists, and medical and chemical researchers, this book will prove a handy reference.
1 Introduction 2 Methods for Estimating Amine Oxidase Activity 3 Distribution and Intracellular Localization of Amine Oxidases 4 Methods of Isolation and Purification of Amine Oxidases 5 Physicochemical Properties of Amine Oxidases 6 Substrate Specificity of Amine Oxidases 7 Inhibition of Amine Oxidase Activity 8 Stimulation of Amine Oxidase Activity 9 Multiplicity of the Amine Oxidases 10 Regulation of Amine Oxidase Activity 11 Biological Functions of the Amine Oxidases 12 Amine Oxidases and Pathology 13 Conclusion References
- No. of pages:
- © Pergamon 1983
- 1st January 1983
- eBook ISBN: