Algal Culturing Techniques is a comprehensive reference on all aspects of the isolation and cultivation of marine and freshwater algae, including seaweeds. It is divided into seven parts that cover history, media preparation, isolation and purification techniques, mass culturing techniques, cell counting and growth measurement techniques, and reviews on topics and applications of algal culture techniques for environmental investigations. Algal Culturing Techniques was developed to serve as both a new textbook and key reference for phycologists and others studying aquatic systems, aquaculture and environmental sciences. Students of algal ecology, marine botany, marine phycology, and microbial ecology will enjoy the hands-on methodology for culturing a variety of algae from fresh and marine waters. Researchers in industry, such as aquaculture, pharmaceutical, foodstuffs, and biotechnology companies will find an authoritative and comprehensive reference.

Key Features

* Sponsored by the Phycological Society of America * Features color photographs and illustrations throughout * Describes culturing methods ranging from the test tube to outdoor ponds and coastal seaweed farms * Details isolation techniques ranging from traditional micropipette to automated flow cytometeric methods * Includes purification, growth, maintenance, and cryopreservation techniques * Highlights methods for estimating algal populations, growth rates, isolating and measuring algal pigments, and detecting and culturing algal viruses * Features a comprehensive appendix of nearly 50 algal culture medium recipes * Includes a glossary of phycological terms


Scientists, industry professionals, and students who study or grow algal cultures for research or commercial purposes. Libraries, aquaculturists, biotechnologists, governmental scientists, etc.

Table of Contents

Contributors Preface 1. Historical Review of Algal Culturing Techniques 2. Freshwater Culture Media 3. Marine Culture Media 4. Trace Metal Ion Buffers and Their Use in Culture Studies 5. Sterilization and Sterile Technique 6. Traditional Microalgae Isolation Techniques 7. Automated Isolation Techniques for Microalgae 8. Purification Methods for Microalgae 9. Isolation and Purification Techniques for Macroalgae 10. Perpetual Maintenance of Actively Metabolizing Microalgal Cultures 11. Long Term Macroalgal Culture Maintenance 12. Cryopreservation Methods for Maintaining Microalgal Cultures 13. Photobioreactors and Fermentors: The Light and Dark Sides of Growing Algae 14. Culturing Microalgae in Outdoor Ponds 15. Mariculture of Seaweeds 16. Counting Cells in Culture Using the Light Microscope 17. Phytoplankton Cell Counting by Flow Cytometry 18. Measuring Growth Rates in Microalgal Cultures 19. Using Cultures to Investigate the Physiological Ecology of Microalgae 20. Analysis of Algal Pigments by High-Performance Liquid Chromatography 21. Endogenous Rhythms and Daylength Effects in Macroalgal Development 22. Viral Contamination of Algal Cultures 23. Control of Sexual Reproduction in Algae in Culture 24. Microalgal Life Cycle Dynamics: Encystment and Excystment 25. Cultures as a Means of Protecting Biological Resources: Ex-situ Conservation of Threatened Algal Species Appendix A: Recipies for Freshwater and Seawater Media Glossary Name Index Subject Index


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© 2005
Academic Press
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"It has been worth the wait. Andersen and the authors have done a tremendous job bringing together many old and new methods in highly readable and easy to follow order...In addition to the older standard methods, it adds many new ones, from automated isolation and counting of algal cells to use of cultures as a way to prevent extinctions of threatened species...the methods chapters, e.g., Andersen and Kawachi on microalgal isolation techniques are very detailed, providing plain, easy to follow, often step-by-step instructions, complete with supplies needed and even sources, on how to do particular tasks...Bob Andersen, the authors, and PSA are to be commended for bringing together so much detailed information in one place. The book is beautifully produced...It definitely needs to be in the lab and/or office of everyone doing research on algae...I highly recommend it." - R.A. Horner, University of Washington, School of Oceanography, Seattle, U.S.A., in HARMFUL ALGAE (2006) "This comprehensive book is a necessity for research scientists who use and maintain algal cultures." CHOICE (October 2005)