Algal Culturing Techniques

Algal Culturing Techniques

1st Edition - December 10, 2004

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  • Editor: Robert A. Andersen
  • eBook ISBN: 9780080456508
  • Hardcover ISBN: 9780120884261

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Algal Culturing Techniques is a comprehensive reference on all aspects of the isolation and cultivation of marine and freshwater algae, including seaweeds. It is divided into seven parts that cover history, media preparation, isolation and purification techniques, mass culturing techniques, cell counting and growth measurement techniques, and reviews on topics and applications of algal culture techniques for environmental investigations. Algal Culturing Techniques was developed to serve as both a new textbook and key reference for phycologists and others studying aquatic systems, aquaculture and environmental sciences. Students of algal ecology, marine botany, marine phycology, and microbial ecology will enjoy the hands-on methodology for culturing a variety of algae from fresh and marine waters. Researchers in industry, such as aquaculture, pharmaceutical, foodstuffs, and biotechnology companies will find an authoritative and comprehensive reference.

Key Features

  • Sponsored by the Phycological Society of America
  • Features color photographs and illustrations throughout
  • Describes culturing methods ranging from the test tube to outdoor ponds and coastal seaweed farms
  • Details isolation techniques ranging from traditional micropipette to automated flow cytometeric methods
  • Includes purification, growth, maintenance, and cryopreservation techniques
  • Highlights methods for estimating algal populations, growth rates, isolating and measuring algal pigments, and detecting and culturing algal viruses
  • Features a comprehensive appendix of nearly 50 algal culture medium recipes
  • Includes a glossary of phycological terms


Scientists, industry professionals, and students who study or grow algal cultures for research or commercial purposes. Libraries, aquaculturists, biotechnologists, governmental scientists, etc.

Table of Contents

  • Contributors
    1. Historical Review of Algal Culturing Techniques
    2. Freshwater Culture Media
    3. Marine Culture Media
    4. Trace Metal Ion Buffers and Their Use in Culture Studies
    5. Sterilization and Sterile Technique
    6. Traditional Microalgae Isolation Techniques
    7. Automated Isolation Techniques for Microalgae
    8. Purification Methods for Microalgae
    9. Isolation and Purification Techniques for Macroalgae
    10. Perpetual Maintenance of Actively Metabolizing Microalgal Cultures
    11. Long Term Macroalgal Culture Maintenance
    12. Cryopreservation Methods for Maintaining Microalgal Cultures
    13. Photobioreactors and Fermentors: The Light and Dark Sides of Growing Algae
    14. Culturing Microalgae in Outdoor Ponds
    15. Mariculture of Seaweeds
    16. Counting Cells in Culture Using the Light Microscope
    17. Phytoplankton Cell Counting by Flow Cytometry
    18. Measuring Growth Rates in Microalgal Cultures
    19. Using Cultures to Investigate the Physiological Ecology of Microalgae
    20. Analysis of Algal Pigments by High-Performance Liquid Chromatography
    21. Endogenous Rhythms and Daylength Effects in Macroalgal Development
    22. Viral Contamination of Algal Cultures
    23. Control of Sexual Reproduction in Algae in Culture
    24. Microalgal Life Cycle Dynamics: Encystment and Excystment
    25. Cultures as a Means of Protecting Biological Resources: Ex-situ Conservation of Threatened Algal Species
    Appendix A: Recipies for Freshwater and Seawater Media
    Name Index
    Subject Index

Product details

  • No. of pages: 592
  • Language: English
  • Copyright: © Academic Press 2005
  • Published: December 10, 2004
  • Imprint: Academic Press
  • eBook ISBN: 9780080456508
  • Hardcover ISBN: 9780120884261

About the Editor

Robert A. Andersen

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  • NIKOLAOSVLACHOS Fri Dec 27 2019

    Very comprehensive yet readable

    This book seems to cover all the bases of algae culture, yet is quite easilly understood by a newcomer to phycology such as myself. I found the descriptions of practical procedure very valuable and well explained/illustrated. The material is often accompanied by historical background of the procedures and algae know-how discussed, making it a more interesting read but also giving a better appreciation of difficulty and/or significance.