Affinity Chromatography and Biological Recognition

Affinity Chromatography and Biological Recognition

1st Edition - January 28, 1983

Write a review

  • Editor: Irwin Chaiken
  • eBook ISBN: 9780323146623

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.

Table of Contents

  • Contributors


    Part I. Introduction

    Molecular Interactions, Affinity Methods, and the Developing Synergism between Affinity Chromatography and Biological Recognition

    Some General Considerations on the Recognition by and of Proteins

    Part II. Molecular Interactions in Biorecognition

    Emerging Concepts in the Mechanism of Action of Membrane Receptors

    Cellular Recognition: Immobilized Synthetic and Natural Glycoconjugates Elicit Specific Cell Adhesion and Post-Adhesion Responses

    Versatility of Calmodulin as a Cytosolic Regulator of Cellular Function

    Effects of Individual Amino Acid Substitutions on the Affinity of Hemoglobin Subunits for Each Other

    Protein Recognition of Nucleic Acid Sequences

    Part III. Characterizing Biomolecular Interactions Using Affinity Methods

    Application of Quantitative Affinity Chromatography to the Study of Protein-Ligand Interactions

    Subunit Exchange Chromatography: Principles and Applications

    Affinity Chromatography Studies of Cooperative Interactions in Neurophysin-Neuropeptide Hormone Complexes

    Quantitative Affinity Chromatography: New Methods for Kinetic and Thermodynamic Characterization of Macromolecular Interactions

    Quantitative Analysis of Immobilized Dye-Protein Interactions

    Biospecific Desorption from Low-Specificity Adsorbents with Emphasis on 10-Carboxydecylamino-Sepharose

    A Study of Multispecific Interactions by Quantitative Affinity Chromatography

    Metabolite-Induced Changes in the Subcellular Distribution of Enzymes: Quantitative Affinity Chromatography in Vivo

    Interaction between Human Hemoglobin and Sulfated Polysaccharides: Identification of the Binding Site and Specificity

    Substrate Recognition by Cyclic AMP-Dependent Protein Kinase: Ligand Effects on Phosphorylation of Hepatic Phenylalanine Hydroxylase

    Studies on the Nature of Metal-Mediated Enzyme Binding to Triazine Dye Affinity Matrices

    Determination of Affinity Constants: Evaluation of Binding Reactions in Aqueous Two-Phase Systems

    Part IV. Affinity Methods—Design and Development

    IMA-Chromatography (Immobilized Ion Affinity Chromatography): Reflections of Methodological Development

    Preparative and Analytical Applications of CDI-Mediated Affinity Chromatography

    New Approaches for the Use of Cyanogen Bromide and Related Cyanylating Agents for the Preparation of Activated Polysaccharide Resins

    Novel Affinity Techniques

    Ultrafiltration Affinity Purification

    Hydrophobic Chromatography and Its Relevance to Biological Recognition

    Recent Advances in High Performance Liquid Affinity Chromatography (HPLAC)

    High Performance Liquid Affinity Chromatography: Silica-Bound Alcohol Dehydrogenase as an Adsorbent for Nucleotides and Other Compounds

    Recent Applications of High Performance Liquid Affinity Chromatography (HPLAC) with Boronic Acid Silica

    High Performance Liquid Affinity Chromatography (HPLAC) with Protein A-Silica

    Preparation of a High Flow Packing Material (Silica Based) for High Performance Affinity Chromatography of Proteins

    Optical Resolution of Racemic Compounds by Means of HPLC on Immobilized Protein Stationary Phases—Theory and Applications

    Rapid Quantitative Analysis Using High Performance Affinity Chromatography Minicolumns

    Kinetic and Diffusional Effects in High Performance Affinity Chromatography

    High Performance Metal Chelate Chromatography

    High Performance Liquid Chromatography with Immobilized Metal Adsorbents

    Synthesis of Trisacryl Sorbents for Metal Chelate Chromatography: Application to Monokine Separation

    Mild Hydrophobic Chromatography

    Protein Adsorption Hysteresis on Hydrophobic Agaroses

    Effect of Matrix on Biospecific Complex Formation

    Preparation of an Active Ester Agarose Derivative Having a Positively Charged Spacer Arm: Enhanced Coupling to Acidic Proteins

    Mersalyl-Trisacryl as a Tool for Ligand Immobilization through Cleavable Mercury-Sulfur Bonds: Applications to Cell Affinity Chromatography

    Colored Sulfonyl Chlorides as Activating Agents for Hydroxylic Matrices

    Study of the Use of Titanium Hydroxide as an Inorganic Support for the Preparation of Affinity Sorbents

    Nylon Tube—A Novel Affinity Matrix Used in the Purification of Receptors, Immunosorption, and Biorecognition Studies

    Site to Site Directed Immobilization of Enzymes with Bis-NAD Analogues

    New Computerized Device for Complete Automation of Affinity Chromatography Separations

    Partition Affinity Ligand Assay (PALA)

    Part V. Antibodies in Affinity Methodology

    Antibodies as in Vivo Diagnostic and Therapeutic Agents

    Affinity Therapy—The Use of Antibodies as Carriers for Chemotherapeutic Drugs

    Use of Monoclonal Antibodies for Diagnosis and Therapy of Tumor Metastases in Lymph Nodes

    The Chemical Nature of Antiviral Serotype Specificity

    The Use of Affinity Chromatography in the Study of Protein Folding

    Immunoassays Based on Specific Bio-Recognition

    DIA-Disperse Dye Immunoassay

    Affinity Chromatography Applied to Radioimmunoassay of Fragment Ε-Related Neoantigen

    System Design for Industrial Scale Purification of High Value Proteins by Immunosorbent Chromatography

    The Selection of Monoclonal Antibodies Suitable for Immunoadsorption

    Affinity Chromatography with Mouse Monoclonal IgM

    An Unusual Monoclonal Antibody Crossreactivity Providing a Method of Purification of an Oriental Hornet Venom Toxin

    Preparation of Immunosorbents with Very Low Non-Specific Binding Properties Using Periodate Oxidized Crosslinked Sepharose

    Tyrosine Phosphate and Tyrosine Sulfate in Proteins

    Part VI. Applications in Separation and Biotechnology

    Historical Perspectives of Purification of Biomolecules

    Some Applications of Bio-Mimetic Dyes

    Applications of Immobilized Boronic Acids

    Use of Paper-Immobilized Phenylboronic Acids for the Measurement of Glycosylated Hemoglobin

    Nucleic Acids and Related Compounds in Affinity Chromatography

    The Use of Heparin-Sepharose for Separation of Various RNA Polymerases and Protein Kinases

    Resolution of Two Forms of Human Antithrombin III by Affinity Chromatography on Immobilized Heparin

    Use of Affinity Chromatography for the Purification of Plasma Proteins

    Studies on Plasma Protein Fractionation with Fatty Acids

    Affinity Chromatography of Interferons—A Novel Application for Calmodulin-Sepharose

    Dextran-Sepharose Affinity Chromatography for Isolation of Chondronectin from Human Plasma

    Covalent Transition-State Affinity Chromatography of Trypsin-Like Proteases

    The Rapid Large Scale Purification of Bacillus Stearothermophilus Glycerokinase by Triazine Dye Affinity Chromatography

    Multiple Peak Formation in the Reversed Phase Liquid Chromatographic Separation of Soybean Trypsin Inhibitor

    N-F Transition of Human Serum Albumin: A Probe by Hydrophobic Interaction Chromatography

    Preliminary Purification of Two Human Blood Cell Hormones by Hydrophobic Interaction Chromatography

    Desulfovibrio desulfuricans Cytochrome c3: Isolation and Purification by Hydrophobic Chromatography

    Hydrophobic Bacterial Surface Properties Are Determined by Pili Which Mediate Attachment to Intestinal Brush Border Membranes

    Immobilized Adriamycin and Carminomycin: Coupling Chemistry and Effects on Survival of L1210 and S180 Clones

    Dextran-Bound Purine Nucleosides—Substrates and Inhibitors of Adenosine Deaminase

    Affinity Binding Used as a Tool to Concentrate Cells for Quantitation and for Bioassays

    Competitive Inhibition Assays for Mycoplasma pneumoniae Binding Components and Mammalian Cell Receptors


Product details

  • No. of pages: 542
  • Language: English
  • Copyright: © Academic Press 1983
  • Published: January 28, 1983
  • Imprint: Academic Press
  • eBook ISBN: 9780323146623

About the Editor

Irwin Chaiken

Ratings and Reviews

Write a review

There are currently no reviews for "Affinity Chromatography and Biological Recognition"