Advances in Planar Lipid Bilayers and Liposomes

Advances in Planar Lipid Bilayers and Liposomes

A Tribute to Marin D. Mitov

1st Edition - May 11, 2013

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  • Editor: Ales Iglic
  • eBook ISBN: 9780124115415

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Description

Advances in Planar Lipid Bilayers and Liposomes volumes cover a broad range of topics, including main arrangements of the reconstituted system, namely planar lipid bilayers as well as spherical liposomes. The invited authors present the latest results of their own research groups in this exciting multidisciplinary field.

Key Features

  • Incorporates contributions from newcomers and established and experienced researchers
  • Explores the planar lipid bilayer systems and spherical liposomes from both theoretical and experimental perspectives
  • Serves as an indispensable source of information for new scientists

Readership

Chemical Engineers, physical chemists, biologists and biochemists, biochemical engineers

Table of Contents

  • Editorial Board

    Preface

    Contributors

    Chapter One. Marin Mitov Lectures: Measuring the Bending Elasticity of Lipid Bilayers

    1 Introduction

    2 Calculating the Curvatures

    3 Volume, Area, and Free Elastic Energy of a Quasi-spherical Vesicle

    4 Experimental Quantities

    5 Closer to the Reality

    6 Formation of Giant Lipid Vesicles

    7 Experimental Setup: Stroboscopic Illumination Module

    8 Data Processing

    9 Conclusion

    Acknowledgments

    References

    Chapter Two. Formation of Curved Membranes and Membrane Fusion Processes Studied by Synchrotron X-ray-Scattering Techniques

    1 Introduction

    2 Synchrotron Light for Membrane Response Analysis

    3 Bending and Packing in Curved Membrane Systems

    4 Membrane Fusion

    5 The Lamellar to Primitive Cubic Phase Transition

    6 The Lamellar to Gyroid Cubic Phase Transition

    7 The Lamellar to Inverse Hexagonal Phase Transition

    8 Conclusion and Perspectives

    Acknowledgments

    References

    Chapter Three. Overview of a Quest for Bending Elasticity Measurement

    1 Introduction

    2 From GUV Fluctuations to Bending Elasticity Measurements

    3 Conclusion

    References

    Chapter Four. Extracting Interaction Parameters and Intrinsic Properties of Membrane from Correlations of Giant Vesicle Shape Fluctuations

    1 Introduction

    2 From the Shape Deformations of Lipid Bilayers to the Flickering Technique

    3 Material and Methods

    4 Extracting Material Parameters from Membrane Conformational Correlation Functions

    5 Outlook

    Acknowledgment

    References

    Chapter Five. Lipid Bilayers and Membranes: Material Properties

    1 Introduction

    2 Material Constants of Bilayers and Membranes: Overview and Discussion of Experimental Data

    3 Concluding Remarks

    Acknowledgment

    References

    Chapter Six. Interaction of Polylysines with the Surface of Lipid Membranes: The Electrostatic and Structural Aspects

    1 Introduction

    2 Materials and Methods

    3 Electrokinetic Measurements in the Liposome Suspension

    4 BP of Membranes

    5 PL Adsorption at the Lipid Monolayers

    6 Isothermal Titration Calorimetry of Liposomes in the Presence of PL

    7 AFM of PLs at the Surface of Bilayer

    8 Interaction of Lysine with Lipid Membranes

    9 Lysine at the Membrane Surface: Analysis by MD

    10 Speculations on PL Interaction with Lipid Bilayers

    Acknowledgments

    References

    Chapter Seven. Developing Cell-Scale Biomimetic Systems: A Tool for Understanding Membrane Organization and Its Implication in Membrane-Associated Pathological Processes

    1 Concept of Membrane Rafts and Their Cellular Functions

    2 Cell-Scale Biomimetic Systems as a Tool to Study Membrane Organization and Functioning

    3 Effect of Sphingolipids on the Formation of Membrane Domains and Their Morphology in Biomimetic Systems

    4 Visualization of Enzyme Activity in Biomimetic Systems

    5 Conclusions and Biological Implications

    Acknowledgments

    References

    Chapter Eight. Carotenoid–Lipid Interactions

    1 Introduction

    2 Characteristics of Carotenoids

    3 Model Membranes

    4 Methods of Investigation

    5 Carotenoid–Lipid Interactions

    References

    Chapter Nine. Theory of Phase Transitions: From Magnets to Biomembranes

    1 Introduction

    2 Phase Transitions and Statistical Mechanics

    3 Theory of Phase Transitions

    4 Effects of the Interaction Potential on the Transition

    5 Application to Biomembranes

    Acknowledgments

    References

    Chapter Ten. Interaction of Cadmium and Mercury Ions with Bilayer Lipid Membranes Containing Channels

    1 Introduction

    2 Materials and Methods

    3 Results and Discussion

    4 Conclusion

    References

    Chapter Eleven. High-Resolution Solid-State NMR of Lipid Membranes

    1 Introduction

    2 Experimental Considerations

    3 Applications

    4 Other Applications and New Directions

    5 Summary

    Acknowledgments

    References

    Chapter Twelve. Thermal Fluctuations of Phospholipid Vesicles Studied by Monte Carlo Simulations

    1 Introduction

    2 Thermal Fluctuations and Elasticity in Two Dimensions

    3 Thermal Fluctuations and Elasticity in Three Dimensions

    4 Discussion

    References

    Subject Index

Product details

  • No. of pages: 382
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: May 11, 2013
  • Imprint: Academic Press
  • eBook ISBN: 9780124115415

About the Serial Editor

Ales Iglic

Ales Iglic
Aleš Iglič received his B.Sc. and Ph.D. degrees in physics and M.Sc. degree in biophysics from the Department of Physics, and the Ph.D. degree in electrical engineering from the Faculty of Electrical Engineering, all from the University of Ljubljana. He is a Full Professor and the Head of Laboratory of Biophysics of the Faculty of Electrical Engineering at University of Ljubljana. His main research interests are in electrostatics, mechanics and statistical physics of lipid nanostructures and biological membranes. He is devoted to higher education, basic research in biophysics and close contacts to clinical practice. Prof. Iglič was visiting scientist and professor at Åbo Academy University in Turku (Finland), Friedrich Schiller University in Jena (Germany) and Czech Technical University in Prague (Czech Republic). He established collaborations with researchers from different universities across the Europe, USA and India and was supervisor of many M.Sc., Ph.D. and postdoctoral students from Slovenia, Czech Republic, Poland, Iran, Bulgaria, Germany, India and Israel. Since 2009 is the editor of Elsevier book series »Advances in Planar Lipid Bilayers and Liposomes« (APLBL).

Affiliations and Expertise

Full Professor and the Head of Laboratory of Biophysics, Faculty of Electrical Engineering, University of Ljubljana, Slovenia

About the Serial Volume Editor

Julia Genova

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