Adult Stem Cells - 1st Edition - ISBN: 9780123736505, 9780080469164

Adult Stem Cells, Volume 419

1st Edition

Serial Volume Editors: Irina Klimanskaya Robert Lanza
eBook ISBN: 9780080469164
Hardcover ISBN: 9780123736505
Imprint: Academic Press
Published Date: 22nd November 2006
Page Count: 576
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Table of Contents

  • Dedication
  • Contributors to Volume 419
  • Preface
  • Foreword
  • Contents of Previous Volumes
  • Section I: Ectoderm
    • [1]. Neural Stem Cell Isolation and Characterization
      • Abstract
      • Introduction
      • Reagents and Instrumentation
      • Methods
      • Acknowledgments
    • [2]. Neural Stem Cells and Their Manipulation
      • Abstract
      • Introduction
      • Adult Niches for Stem Cells In Vivo
      • In Vitro Manipulation of NSCs
      • Conclusions and Projections
      • Sample Protocols for the Culture and Characterization of NSCs
      • Acknowledgments
    • [3]. Retinal Stem Cells
      • Abstract
      • Introduction
      • Materials and Methods
      • Overview
    • [4]. Epithelial Skin Stem Cells
      • Abstract
      • Introduction to Skin Epithelial Stem Cells
      • Epithelial Skin Organization and the Hair Cycle
      • Characteristics of Skin Epithelial Stem Cells
    • [5]. Dental Pulp Stem Cells
      • Abstract
      • Introduction
      • Identification of Dental Pulp Stem Cells
      • Isolation of DPSCs
      • Differentiation of DPSCs
  • Section II: Mesoderm
    • [6]. Postnatal Skeletal Stem Cells
      • Abstract
      • Introduction
      • Skeletal Stem Cells and Mesenchymal Stem Cells
      • Defining a Skeletal Stem Cell
      • Origin and Nature of Skeletal Stem Cells
      • Plasticity of BMSCs
      • Role of Skeletal Stem Cells in Disease
      • Molecular Engineering
      • Potential Use of Skeletal Stem Cells in Tissue Engineering and Regenerative Medicine
      • Establishment of Nonclonal Populations of BMSCs In Vitro
      • Establishment of Clonal Populations of BMSCs In Vitro
      • Determination of Colony-Forming Efficiency
      • In Vitro Differentiation of BMSCs
      • In Vivo Differentiation of BMSCs
      • Acknowledgments
    • [7]. Hematopoietic Stem Cells
      • Abstract
      • Introduction
      • Identification and Enrichment of HSCs
      • Functional Characterization of Candidate HSCs
      • Long-Term Culture of Candidate HSCs and Progenitors
      • Acknowledgments
    • [8]. Hemangioblasts and Their Progeny
      • Abstract
      • Introduction
      • Ex Vivo Expansion of CD133+ Cells
      • Hematopoietic and Endothelial Differentiation of Single Cell-Derived EGFP+ Clones
      • Phenotypical and Functional Studies of CD133-Derived Cells during Ex Vivo Expansion and Differentiation
      • Materials
      • Reagents
      • Methods
    • [9]. Kidney Epithelial Cells
      • Abstract
      • Introduction
      • Analysis of Steady State Tubular Epithelium Maintenance
      • Regeneration of Experimentally Injured Kidney Tubules
      • Extratubular Progenitor Cells
      • Culture of Human Tubular Progenitor Cells
    • [10]. Ovarian Germ Cells
      • Abstract
      • Introduction
      • Origin of Primordial Germ Cells
      • Formation of Human Fetal Oocytes and Primordial and Primary Follicles
      • Immune System-Related Cells and Oogenesis in Fetal Ovaries
      • Fetal Programming of Follicular Renewal during Adulthood
      • “Ovary within the Ovary” Pattern and Thy-1 Differentiation Protein
      • Cessation of Adult Oogenesis
      • Oogenesis and Follicular Renewal in Adult Ovaries
      • Working Hypothesis on the Role of the Gonadal Environment
      • Differentiation of Stem Cells and Tissue Control System Theory
      • Potential Treatment of Ovarian Infertility by Producing New Primary Follicles In Vivo
      • Mystery of the Origin of Oocytes in Adult Mammalian Ovaries
      • Development of Distinct Cell Types from Totipotent Ovarian Stem Cells In Vitro
      • Ovarian Stem Cell Culture Protocol
      • Ovarian Stem Cell Cultures and Autologous Treatment of POF
      • Potential Pitfalls
      • Autologous Ovarian Stem Cells and Treatment of Degenerative Diseases
      • Conclusion
      • Acknowledgment
    • [11]. Spermatogonial Stem Cells
      • Abstract
      • Introduction
      • Spermatogonial Stem Cells
      • SSC Transplantation
      • SSC Culture
      • Implications
      • Acknowledgments
  • Section III: Endoderm
    • [12]. Stem Cells in the Lung
      • Abstract
      • Introduction
      • Anatomical and Cellular Diversity of Adult Lung
      • Stem Cell Phenotypes and Niches in Adult Lung
      • In Vivo Injury Models of the Lung
      • Ex Vivo Epithelial Tracheal Xenograft Model to Study Stem Cell Expansion in Proximal Airway
      • In Vitro Colony-Forming Efficiency Assay to Characterize Stem Cell Populations in Conducting Airway Epithelium
      • Models to Study Stem/Progenitor Cells of Airway Submucosal Glands
      • Acknowledgments
    • [13]. Pancreatic Cells and Their Progenitors
      • Abstract
      • Introduction
      • Pancreas Development
      • Origins of Beta Cells During Postnatal Life
      • Summary and Perspective
      • Methods: Design of a Lineage-Tracing Experiment in Mice
    • [14]. Intestinal Epithelial Stem Cells and Progenitors
      • Abstract
      • Introduction
      • Assaying Intestinal Stem Cells, Progenitors, and Their Lineages In Vivo
      • Intestinal Epithelium In Vitro
  • Section IV: Extraembryonic and Perinatal Stem Cells
    • [15]. Trophoblast Stem Cells
      • Abstract
      • Introduction
      • Preparations for Culture of TS Cells
      • Preparation of Feeder Cell Stocks
      • Establishment of TS Cell Lines
      • Passage and Maintenance of TS Cells
      • Freezing and Thawing of TS Cells
    • [16]. Pluripotent Stem Cells from Germ Cells
      • Abstract
      • Introduction
      • Germ Cell Development
      • Embryonic Germ Cell Derivation
      • Characterization of EG Cultures
      • Embryoid Body Formation and Analysis
      • Embryoid Body-Derived Cells
      • Acknowledgments
    • [17]. Amniotic Fluid and Placental Stem Cells
      • Abstract
      • Introduction
      • Amniotic Fluid and Placenta in Developmental Biology
      • Amniotic Fluid and Placenta for Cell Therapy
      • Isolation and Characterization of Progenitor Cells
      • Differentiation of Amniotic Fluid- and Placenta-Derived Progenitor Cells
      • Amniotic Fluid and Placental Differentiation Protocols
      • Conclusion
    • [18]. Cord Blood Stem and Progenitor Cells
      • Abstract
      • Introduction: Cord Blood Transplantation
      • Hematopoietic Stem and Progenitor Cells
      • Endothelial Progenitor Cells
      • Methodologies for Assessing Hematopoietic Progenitor and Stem Cells, as Well as Endothelial Progenitor Cells, Present in Human Cord Blood
      • Methods for Enumerating Human Myeloid Progenitor Cells in Umbilical Cord Blood by In Vitro Colony Formation
      • Methods for Enumerating Human Hematopoietic Stem Cells in Umbilical Cord Blood
      • Methods for Enumerating Endothelial Progenitor Cells in Umbilical Cord Blood
      • Uses of Preceding Assays
  • Author Index
  • Subject Index


This is the second of three planned volumes in the Methods in Enzymology series on the topic of stem cells. This volume is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world. The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and troubleshooting of these state-of-the-art technologies in other laboratories.

Key Features

  • Provides complete coverage spanning from derivation/isolation of stem cells, and including differentiation protocols, characterization and maintenance of derivatives and tissue engineering
  • Presents the latest most innovative technologies
  • Addresses therapeutic relevance including FDA compliance and tissue engineering


Biochemists, cell and molecular biologists, and bioengineers working with stem cells.


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© Academic Press 2006
Academic Press
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About the Serial Volume Editors

Irina Klimanskaya Serial Volume Editor

Affiliations and Expertise

Advance Cell Technology, Worcester, MA, USA

Robert Lanza Serial Volume Editor

Robert Lanza, M.D. is currently Chief Scientific Officer at Advanced Cell Technology, and Adjunct Professor of Surgical Sciences at Wake Forest University School of Medicine. He has several hundred scientific publications and patents, and over 30 books, including Principles of Tissue Engineering (1st through 4th Editions), Methods of Tissue Engineering, Principles of Cloning (1st and 2nd Editions), Essentials of Stem Cell Biology (1st and 2nd Editions), XENO, Yearbook of Cell & Tissue Transplantation, One World: The Health & Survival of the Human Species in the 21st Century (as editor, with forewords by C. Everett Koop and former President Jimmy Carter), and Medical Science & the Advancement of World Health. Dr. Lanza received his B.A. and M.D. degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. He is a former Fulbright Scholar, and studied as a student in the laboratory of Richard Hynes (MIT), Jonas Salk (The Salk Institute), and Nobel laureates Gerald Edelman (Rockefeller University) and Rodney Porter (Oxford University). He also worked closely (and coauthored a series of papers) with the late Harvard psychologist B.F. Skinner and heart transplant pioneer Christiaan Barnard. Dr. Lanza's current area of research focuses on the use of stem cells in regenerative medicine.

Affiliations and Expertise

Advanced Cell Technology, MA, USA