The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 285 volumes have been published (all of them still in print) and much of the material is relevant even today-truly an essential publication for researchersin all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Prokaryotic ABC Transporters * Eukaryotic ABC Transporters * Nonmammalian ABC Transport Systems * Mammalian P-Glycoproteins * Multidrug Resistance Associated Protein * Cystic Fibrosis Transmembrane Conductance Regulator * Sulfonylurea Receptor * Intracellular ABC Transporters


Biochemists, molecular biologists, physiologists, pharmacologists, geneticists, immunologists, and microbiologists.

Table of Contents

Prokaryotic ABC Transporters: H. Nikaido and J.A. Hall, Overview of Bacterial ABC Transporters. J.A. Hall, A.L. Davidson, and H. Nikaido, Preparation and Reconstitution of Membrane-Associated Maltose Transporter Complex of Escherichia coli. C.H. Panagiotidis and H.A. Shuman, Maltose Transport in Escherichia coli: Mutations That Uncouple ATP Hydrolysis from Transport. W. Boos, Binding Protein-Dependent ABC Transport System for Glycerol 3-Phosphate of Escherichia coli. F.Zhang, J.A. Sheps, and V. Ling, Structure-Function Analysis of Hemolysin B. P. Delepelaire, Erwinia Metalloprotease Permease: Aspects of Secretion Pathway and Secretion Functions. M. Kuroda, H. Bhattacharjee, and B.P. Rosen, Arsenical Pumps in Prokaryotes and Eukaryotes. Eukaryotic ABC Transporters: J.M. Croop, Evolutionary Relationships among ABC Transporters. R. Allikmets and M. Dean, Cloning of Novel ABC Transporter Genes. D. Taglicht and S. Michaelis, Saccharomyces cerevisiae ABC Proteins and Their Relevance to Human Health and Disease. A. Nonmammalian ABC Transport Systems: R. Dudler and M. Sidler, Arabidopsis MDR Genes: Molecular Cloning and Protein Chemical Aspects. S. Volkman and D. Wirth, Functional Analysis of pfmdr1 Gene of Plasmodium falciparum. M. Ouellette, A. Haimeur, K. Grondin, D. Légaré, and B. Papadopoulou, Amplification of ABC Transporter Gene pgpA and of Other Heavy Metal Resistance Genes in Leishmania tarentolae and Their Study by Gene Transfection and Gene Disruption. G.L. Nijbroek and S. Michaelis, Fun


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© 1998
Academic Press
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@from:Praise for the Volume @qu:"...the book describes techniques so diverse that they include all major laboratory approaches of modem biochemistry, cell biology, and molecular biology. It provides step-by-step protocols and overviews in the field that also allow newcomers to obtain an orientation over a wide spectrum of aspects... The content is very carefully edited for clarity and precision of the described procedures. Anyone in possession of the equipment needed will be able to reproduce the described conditions and methods." @source:--DOODY'S PUBLISHING REVIEWS @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work."